The dataset can be downloaded from the following link: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE276251. It consists of control and high glucose-treated zebrafishes. The tasks are as follows:
- Filter out low-quality cells.
- Perform clustering analysis on both datasets (no need to annotate cells).
- Integrate the control and high glucose-treated datasets.
- Identify differentially expressed genes (DEGs) between the control and treated zebrafishes for each cell cluster (0, 1, 2, etc.)