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nextflow.config
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nextflow.config
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manifest {
author = 'Sherrie Wang (nextflow pipeline)/Kevin Kuchinski (FluViewer), adapted by Sherrie Wang'
name = 'BCCDC-PHL/hcv-nf'
version = '1.0.1'
description = 'BCCDC-PHL HCV genotyping pipeline'
mainScript = 'main.nf'
nextflowVersion = '>=20.01.0'
}
params {
profile = false
cache = ''
outdir="results"
mode = "assemble"
illumina_suffixes = ['*_R{1,2}_001', '*_R{1,2}', '*_{1,2}' ]
fastq_exts = ['.fastq.gz', '.fq.gz', '.fastq', '.fq']
fastq_search_path = makeFastqSearchPath( illumina_suffixes, fastq_exts )
adapters = "${baseDir}/assets/primers_adapter.fa"
refhcv = "${baseDir}/assets/1_AJ851228.fa"
corebed = "${baseDir}/assets/amplicon_core.bed"
ns5bbed = "${baseDir}/assets/amplicon_ns5b.bed"
minid = 95
mincov = 95
db_name = "nt"
}
def makeFastqSearchPath ( illumina_suffixes, fastq_exts ) {
def fastq_search_path = []
for (suffix in illumina_suffixes){
for (ext in fastq_exts) {
fastq_search_path.add(params.fastq_input.toString() + '/' + suffix.toString() + ext.toString())
}
}
return fastq_search_path
}
profiles {
conda {
process.conda = "$baseDir/environments/environment.yml"
if (params.cache){
conda.cacheDir = params.cache
}
}
}
process {
withName: segcov {
conda = "$baseDir/environments/plotRenvironment.yml"
}
withName: plotdepthdb {
conda = "$baseDir/environments/plotRenvironment.yml"
}
withName: mixscan {
conda = "$baseDir/environments/freyjaenvironment.yml"
}
withName: mafftraxmltree {
shell = ['/bin/bash', '-uo','pipefail' ]
}
withName: findamplicon {
shell = ['/bin/bash', '-uo','pipefail' ]
}
withName: blastconsensus {
shell = ['/bin/bash', '-uo','pipefail' ]
}
}