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Identification of novel effectors among unique fungal genes (fusarium oxysporum).

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EffectorScan-prototype

Prototype code for identifying novel effectors from fusarium oxysporum unique genes. It starts from the raw fasta files 'core_Fo_genes_with_SP.fasta' and 'lycopersici_unique_with_SP.fasta' contained in a directory named 'raw_fasta'. The code is a mixture of bash scripts and Quarto notebooks (with R script alternatives available for the Quarto notebooks). These should be run in order and contain documentation within.

It is reccomended this workflow is ran on a cluster with a GPU to speed up the computational and creation of structure models using colabfold.

The analysis requires two key pieces of software to be installed (in addition to a standard Rstudio set-up):

  • ColabFold installation instructions can be found here
  • FoldSeek installation instructions and tutorials can be found here

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Identification of novel effectors among unique fungal genes (fusarium oxysporum).

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