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Bacterial Media recipes used in the laboratory

SOLID MEDIA

10% Trypticase Soy Agar + cycloheximide

For general isolation of bacteria (standard medium for bacterial isolation from plants)

Ingredient Amount
Trypticase Soy Broth 3 grams
Agar 15 g
deionized H2O 1 Liter

NOTES: Mix and heat to dissolve all ingredients. Autoclave, and cool to pouring temperature. Before pouring add: Cycloheximide (200 mg/L) as 10mL of a 1g/50mL 90% EtOH stock solution.