For general isolation of bacteria (standard medium for bacterial isolation from plants)
Ingredient | Amount |
---|---|
Trypticase Soy Broth | 3 grams |
Agar | 15 g |
deionized H2O | 1 Liter |
NOTES: Mix and heat to dissolve all ingredients. Autoclave, and cool to pouring temperature. Before pouring add: Cycloheximide (200 mg/L) as 10mL of a 1g/50mL 90% EtOH stock solution.