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MicroScope_Cam_class102.py
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MicroScope_Cam_class102.py
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#MicroScope CMOS Camera Viewer with Scale-Bar ... class102 is version for CMOS camera 2592x1944 @ 25fps
import os
import tkinter as tk
from tkinter import ttk
from tkinter import filedialog
import cv2
from PIL import Image, ImageTk, ImageOps #
class Application(tk.Frame):
def __init__(self, master):
super(Application, self).__init__(master)
self.pack()
self.master.geometry("1350x690+0+0") # TK window size(width x height & Left Top)
str_prog_name = os.path.basename(__file__) # get present program name
self.master.title( str_prog_name )
#Initialize view, camera CMOS spec:2592x1944 @ 25fps 1/1.8" Color IMX CMOS Image Sensor 3840x2160 @ 25fps;2592x1944 @ 25fps Pixel size=2×2μm
self.view_width = 864
self.view_height = 648
self.view_magnify = 1/4 # Camera to Picture ratio 3:1 .... change 1/3->1/4 for speed_up
self.camera_id = 0 # 0=PC_Cam 1=External_USB_Cam
self.frame_rate = 5 #25fps camera
self.disp_id = None #for picture animation
# Canvas
self.canvas = tk.Canvas(root, width=self.view_width, height=self.view_height, bg='gray')
self.canvas.pack(side=tk.LEFT,expand=True,anchor=tk.NW)
# on Canvas, bind mouse event（Left Click)
self.canvas.bind('<Button-1>', self.canvas_click)
self.canvas.pack(expand = True, fill = tk.BOTH)
#Default save file
file_name1 = "Capture.jpg"
self.typelist1 = [("Capture.jpg", ".jpg"),]
#self.ini_dir = os.path.dirname(__file__) # get present program directory
self.ini_dir =r'C:\Users\taniyama\Desktop'
self.file_path = os.path.join(self.ini_dir, file_name1)
#print (self.file_path)
self.count = 0
# Initialize show scale profile
self.show_scale = True # True= Show Scale , False= Hide Scale
self.relaylens_magnify = 0.53 # Zoom lens x0.35 -> x0.70 maxsize 0.7-> 0.53 #size is ?????
self.str_lens_magnify = 'x10' # Objective Lens x10 Default
self.lens_magnify = 10.0 # Objective Lens x10 Default
self.str_length = '100um' # Scale length
self.length = 100.0 # Scale length
self.pixel_length = 2.0 # 2.0um/Pixel
self.x0 = 350
self.y0 = 100
# Preset Color for Button(tkinter)
self.color_green = str('#ccffaa') #green
self.color_gray = str('#f2f2f2') #gray(Back ground color)
self.color_red = str('#ffaacc') #red
# Preset Color for Scale(opencv)
self.white = (255,255,255) #Color order is BGR on cv2(opencv)
self.red = (0,0,255) #BGR #RGB(255,0,0)
self.aqua = (255,255,0) #BGR #RGB(0,255,255)
self.green = (0,255,0)
self.black = (0,0,0)
self.color = self.white
self.str_color = 'white'
# frame1
frame1 = tk.Frame(root, bd=2, pady=5, padx=5)
frame1.pack(side=tk.RIGHT,expand=True,anchor=tk.NE)
#camera operation
self.btn_live = tk.Button(frame1, text='Play//Pause', command=self.btn_click, width=10, height=2, background=self.color_green)
self.btn_live.grid(row=0, column=0, padx=5, pady=5)
btn_exit = tk.Button(frame1, text='Exit', command=root.quit, width=10, height=2)
btn_exit.grid(row=0, column=1, padx=5, pady=5)
column_0 = ('0', '1', '2') #0:WebCam 1:Microscope USB Cam
btn_camera = tk.Button(frame1, text='Change Camera', command=self.Set_Camera(), width=15, height=2) # run in advance
btn_camera.grid(row=1, column=0, padx=5, pady=5)
self.combobox_0 = ttk.Combobox(frame1, height=1, width=10, justify='center', values=column_0)
self.combobox_0.insert(0, '0') #1:Microscope USB Cam
self.combobox_0.grid(row=1, column=1, padx=5, pady=5)
#Save picture
label_save_filename = tk.Label(frame1, text='Show save file path.')
label_save_filename.grid(row=2, column=0, padx=5, pady=5)
self.save_filename = tk.Text(frame1, height=4,width=25)
self.save_filename.grid(row=3, column=0, padx=5, pady=5)
self.save_filename.insert(tk.END, str(self.file_path))
btn_Set_Plot1 = tk.Button(frame1, text='Set file path', command=self.Set_File_Path, width=15, height=1)
btn_Set_Plot1.grid(row=4, column=0, padx=5, pady=5)
self.btn_save = tk.Button(frame1, text='Save Image File', command=self.Caputure_Image, width=15, height=2)
self.btn_save.grid(row=5, column=0, padx=5, pady=5)
#Show scale
btn_scale = tk.Button(frame1, text='Scale on/off', command=self.Show_Scale, width=15, height=1)
btn_scale.grid(row=6, column=0, padx=5, pady=5)
#Set Objective Lens
lbl_1 = tk.Label(frame1, text='Set Objective Lens')
lbl_1.grid(row=7, column=0, padx=5, pady=5)
column_1 = ('x5', 'x10', 'x20', 'x50', 'x100')
self.combobox_1 = ttk.Combobox(frame1, height=1, width=10, justify='center', values=column_1)
self.combobox_1.insert(0, self.str_lens_magnify)
self.combobox_1.bind('<<ComboboxSelected>>', self.Set_Objective_Lens)
self.combobox_1.grid(row=7, column=1, padx=5, pady=5)
#Set Scale-Bar Length
lbl_2 = tk.Label(frame1, text='Set Length')
lbl_2.grid(row=8, column=0, padx=5, pady=5)
column_2 = ('10um', '20um', '50um', '100um', '200um','500um','1000um')
self.combobox_2 = ttk.Combobox(frame1, height=1, width=10, justify='center', values=column_2)
self.combobox_2.insert(0, self.str_length)
self.combobox_2.bind('<<ComboboxSelected>>', self.Set_Length)
self.combobox_2.grid(row=8, column=1, padx=5, pady=5)
#Set Scale-Bar Color
column_3 = ('white', 'green', 'aqua', 'black', 'red')
lbl_2 = tk.Label(frame1, text='Set Color')
lbl_2.grid(row=9, column=0, padx=5, pady=5)
self.combobox_3 = ttk.Combobox(frame1, height=1, width=10, justify='center', values=column_3)
self.combobox_3.insert(0, self.str_color)
self.combobox_3.bind('<<ComboboxSelected>>', self.Set_Color)
self.combobox_3.grid(row=9, column=1, padx=5, pady=5)
#Load Camera driver configration
btn_Config = tk.Button(frame1, text='Set Camera Configuration', command=self.Set_Config, width=20,height=2, background=self.color_green)
btn_Config.grid(row=10, column=0, padx=5, pady=5)
#mouse click event
label_start_x = tk.Label(frame1, text='mouse_pos_x')
label_start_x.grid(row=11, column=0)
label_start_y = tk.Label(frame1, text='mouse_pos_y')
label_start_y.grid(row=12, column=0)
self.en_x0 = tk.Entry(frame1, width=10, justify='right')
self.en_x0.grid(row=11, column=1)
self.en_x0.insert(tk.END, self.x0)
self.en_y0 = tk.Entry(frame1, width=10, justify='right')
self.en_y0.grid(row=12, column=1)
self.en_y0.insert(0, self.y0)
#Notes
label_save_filename = tk.Label(frame1, text='Image View is shown in 1/4 scale.')
label_save_filename.grid(row=13, column=0, padx=5, pady=5)
label_save_filename = tk.Label(frame1, text='Move the Scale to where mouse is clicked.')
label_save_filename.grid(row=14, column=0, padx=5, pady=5)
def canvas_click(self, event):
'''Canvas mouse click event'''
self.x0 = int(event.x / self.view_magnify)
self.y0 = int(event.y / self.view_magnify)
self.en_x0.delete(0,'end')
self.en_y0.delete(0,'end')
self.en_x0.insert(tk.END, self.x0)
self.en_y0.insert(tk.END, self.y0)
return
def btn_click(self):
'''Play//Pause button '''
if self.disp_id is None:
# 動画を表示
self.disp_image()
self.btn_live.configure(bg = self.color_red) # live button color changed
self.btn_save.configure(bg = self.color_gray) # save button color changed
else:
# 動画を停止
self.after_cancel(self.disp_id)
self.disp_id = None
self.btn_live.configure(bg = self.color_gray) # live button color changed
self.btn_save.configure(bg = self.color_green) # save button color changed
return
def disp_image(self):
'''Show image on Canvas'''
# make image
self.make_image()
# canvas size
#canvas_width = self.canvas.winfo_width()
#canvas_height = self.canvas.winfo_height()
canvas_width = self.view_x
canvas_height = self.view_y
# Resize image to Canvas with original aspect ratio.
pil_image = ImageOps.pad(self.pil_image, (canvas_width, canvas_height))
# change PIL.Image to PhotoImage
self.photo_image = ImageTk.PhotoImage(image=pil_image)
# canva image
self.canvas.create_image(
canvas_width / 2, # location X&Y(Center of Canvas)
canvas_height / 2,
image=self.photo_image # image data
)
# show disp_image after 10msec
self.disp_id = self.after(10, self.disp_image)
return
def make_image(self):
# OpenCV to Canvas
ret, frame = self.capture.read()
if self.show_scale:
#size
line_thickness = 6
line_height= 20
font_size = 2.0
text_length = 300
#Write Scale text
cv2.putText(img=frame, text=self.str_length, org=(self.x0 - text_length, self.y0), fontFace=cv2.FONT_HERSHEY_SIMPLEX,
fontScale=font_size, color=self.color, thickness=line_thickness, lineType=cv2.LINE_AA)
#Draw Scale image length
self.multi_factor = self.lens_magnify * self.relaylens_magnify
pixels = self.length / self.pixel_length * self.multi_factor
length= int(pixels)
x_loc = self.x0
y_loc = self.y0
cv2.line(frame, (x_loc, y_loc), (x_loc+length, y_loc), self.color,thickness=line_thickness)
cv2.line(frame, (x_loc, y_loc), (x_loc, y_loc-line_height), self.color,thickness=line_thickness)
cv2.line(frame, (x_loc+length, y_loc), (x_loc+length, y_loc-line_height), self.color,thickness=line_thickness)
else:
pass
# BGR→RGB
#cv_image = cv2.cvtColor(frame, cv2.COLOR_BGR2RGB)
if frame.ndim == 2: # mono
cv_image = frame
if frame.shape[2] == 1: # mono (8bit)
cv_image = frame
elif frame.shape[2] == 3: # Color(24bit)
cv_image = cv2.cvtColor(frame, cv2.COLOR_BGR2RGB)
elif frame.shape[2] == 4: # Color with Transparent(32bit)
cv_image = cv2.cvtColor(frame, cv2.COLOR_BGRA2RGBA)
pil_image = Image.fromarray(cv_image)
self.pil_image = pil_image
return
def Set_File_Path(self):
#20221108 add defaultextension = "" ....automatically add extension
filename=filedialog.asksaveasfilename(initialdir=self.ini_dir, filetypes=self.typelist1, title="Set File_path", defaultextension = "")
if filename == "":
return
else:
self.file_path = filename
self.f_root, self.ext = os.path.splitext(self.file_path)
self.ini_dir = self.f_root
self.save_filename.delete('1.0','end') # Delete all
self.save_filename.insert(tk.END, str(self.file_path))
print('Set File_path =' , os.path.abspath(self.file_path))
return
def Caputure_Image(self):
'''Save picture'''
self.pil_image.save(self.file_path)
#self.create_modeless_dialog() # 2022/12/21 delete
tk.messagebox.showinfo(title="Image file saved", message="Image file saved.")
#self.pil_image.save('{}_{}.{}'.format(self.base_path, self.count, self.ext))
#self.count += 1
return
def Set_Camera(self):
try: # button color changed
self.btn_live.configure(bg = self.color_green) # live button color changed
self.btn_save.configure(bg = self.color_gray) # save button color changed
self.camera_id = int(self.combobox_0.get()) # camera_id
except:
pass
# Pause Camera and Change Camera
if self.disp_id is None:
pass
else:
# Pause camera
self.after_cancel(self.disp_id)
self.disp_id = None# Pause
try:
# release Camera
self.capture.release()
except:
pass
# Open Camera
self.capture = cv2.VideoCapture(self.camera_id)
#self.frame_prf_0=self.capture.get(cv2.CAP_PROP_FRAME_WIDTH) # get camera profile
#self.frame_prf_1=self.capture.get(cv2.CAP_PROP_FRAME_HEIGHT)
self.frame_prf_0 = 2592 #force write
self.frame_prf_1 = 1944
str_text = 'Camera_'+str(self.camera_id)+'\n'+'Width :'+str(self.frame_prf_0)+'\n'+'Height :' + str(self.frame_prf_1)
tk.messagebox.showinfo(title="Camera connected", message=str_text)
#camera Re-open with "cv2.CAP_DSHOW"
self.capture = cv2.VideoCapture(self.camera_id,cv2.CAP_DSHOW)
try:
self.capture.set(cv2.CAP_PROP_FRAME_WIDTH,self.frame_prf_0)
self.capture.set(cv2.CAP_PROP_FRAME_HEIGHT,self.frame_prf_1)
self.capture.set(cv2.CAP_PROP_FPS,self.frame_rate)
self.view_x = int(self.frame_prf_0 * self.view_magnify)
self.view_y = int(self.frame_prf_1 * self.view_magnify)
except:
self.capture.set(cv2.CAP_PROP_FRAME_WIDTH, 2592) # Force write frame size without using camera profile.
self.capture.set(cv2.CAP_PROP_FRAME_HEIGHT,1944) # Force write frame size without using camera profile.
self.capture.set(cv2.CAP_PROP_FPS,5) # Force write frame size without using camera profile.
self.view_x = int(2592 * self.view_magnify)
self.view_y = int(1944 * self.view_magnify)
if self.view_y == 0.0:
aspect_ratio = self.view_width / self.view_height
else:
aspect_ratio = self.view_x / self.view_y
if self.view_x > self.view_width:
self.view_magnify = self.view_magnify*self.view_width/self.view_x
self.view_x = self.view_width
self.view_y = int(self.view_width / aspect_ratio)
if self.view_y > self.view_height:
self.view_magnify = self.view_magnify*self.view_height/self.view_y
self.view_y = self.view_height
self.view_x = int(self.view_height * aspect_ratio)
return
def Set_Objective_Lens(self,event):
name = self.combobox_1.get()
dictionary = {'x5':5.0, 'x10':10.0, 'x20':20.0, 'x50':50.0, 'x100':100.0}
self.str_lens_magnify = name
self.lens_magnify = dictionary[name]
return
def Set_Length(self,event):
name = self.combobox_2.get()
dictionary = {'10um':10.0, '20um':20.0, '50um':50.0, '100um':100.0, '200um':200.0,'500um':500.0,'1000um':1000.0}
self.str_length = name
self.length = dictionary[name]
return
def Set_Color(self,event):
name = self.combobox_3.get()
dictionary = {'white':self.white, 'green':self.green, 'aqua':self.aqua, 'black':self.black, 'red':self.red }
self.str_color = name
self.color = dictionary[name]
return
def Set_Config(self):
self.capture.set(cv2.CAP_PROP_SETTINGS,1)
#self.caputre.set(cv2.CAP_PROP_AUTO_EXPOSURE,3) #auto mode
#self.caputre.set(cv2.CAP_PROP_AUTO_EXPOSURE,1) #manual mode
#self.caputre.set(cv2.CAP_PROP_EXPOSURE,desired_exposure_value) #exposure_value ...0->1s -1->500ms,,-13->122.1us
return
# Show scale according to "On/Off" button.
def Show_Scale(self):
self.show_scale = not self.show_scale
return
if __name__ == "__main__":
root = tk.Tk()
app = Application(master = root)
app.mainloop()