DNA Cauldron provides a generic cloning simulation framework to predict constructs sequences and detect assembly flaws. It can simulate Golden-Gate, with extra features like enzyme or connector parts autoselection, and other methods like Gibson Assembly, LCR Assembly, BASIC assembly, BioBrick assembly. It supports single and combinatorial assemblies, hierarchical assemblies, and produces nice reports for traceability and troubleshooting.
PIP installation:
pip install dnacauldronWeb documentation:
https://edinburgh-genome-foundry.github.io/DnaCauldron/
Github Page:
https://github.com/Edinburgh-Genome-Foundry/DnaCauldron
Live demo:
http://cuba.genomefoundry.org/simulate_gg_assemblies
License: MIT, Copyright Edinburgh Genome Foundry
DNA Cauldron is part of the EGF Codons synthetic biology software suite for DNA design, manufacturing and validation.