PG_def.hoc

begintemplate PGcell
public soma, dend, gemmshaft, gemmbody
public AMPAr, spiketimes, dendspike, spikecount

create soma, dend, gemmshaft, gemmbody		
objref AMPAr, spiketimes, dendspike, spikecount, dendcount

proc parameter() { 

    AMPAtau		= 5.5		// ms
    Erev		= 0		    // mV
	shell_depth = 0.2       // 
		
		RM	= 20e3  	// ohm.cm2; 20!!!   
        EL 	= -65		// -65 mV !!!
	    
		ENA =  45       // 50
        EK  = -80	    // -75/-80 !!!
	    ECA = 100
		
	    gNa_Soma = 50e-3  // 40  !!!
	    gNa_Dend = 20e-3  // 20
	       Sh_Na =   5    // 20  !!!    
		   
	    gKdr_Soma = 20e-3  // 20
	    gKdr_Dend = 5e-3   //  5 !!!	
	
	    gA_Soma = 10e-3    //  10 !!!
	    gA_Dend = 30e-3    //  30
	       Sha_A =     0   //  9.9 / 0  !!!
	       Shi_A =   -20   //  5.7 / 0  !!!
		   k_tauH  = 2.5   // 1/2.5
           sh_tauH =   0   // 0/-20

	    gH_Dend = 0.2e-3   // 0.2 !!!
		    
	    gM_Soma = 1.0e-3   // 1 !!!
	    gM_Dend = 0.0e-3   // 0		   
	   
	    gKCa_Soma  = 0.0e-3   // 
	    gKCa_Dend  = 2.0e-3   // 2.0!!! Change this will change the number of bursts!!!
	   
	    gCapn_Soma = 0.0e-3		   
	    gCapn_Dend = 1.0e-3	 //  1.0 !!!
	    
		gCaT_Soma = 0.0e-3
	    gCaT_Dend = 3.0e-3   //   5 |5 |5 |    5 / 3 /5
	       Sha_T  =  -15     //  -13|-8|-13|  -10/-13/0
		   Shi_T  =    0     //    3|0 |0     8/ 5 /5  % Posive shift makes burst firing !!!
	       K_tauH =  1.0
		   
		gcan_Dend = 0.0e-3   // 1.5 !!!	
	   
}


proc celldef() {
topol()
subsets()
segments()
geometry()
biophysics($1)
}

proc topol() {											
	connect dend(0), soma(1)
	connect gemmshaft(0), dend(1)
	connect gemmbody(0), gemmshaft(1)
}

// create subsets	
objref pg_all, pgdendgemm, spine
proc subsets() {
	// gemmules (body & shaft)
    spine = new SectionList()	 
	forsec "gemm" spine.append()
	
	// dendrites & gemmules (body & shaft)
	pgdendgemm = new SectionList()
	forsec "dend" pgdendgemm.append()
	forsec "gemm" pgdendgemm.append()	

	// all pg sections
	pg_all = new SectionList()
	forsec pgdendgemm pg_all.append()
	soma pg_all.append()
} 
proc segments() {
	soma.nseg = 1
	dend.nseg = 1
	gemmshaft.nseg = 1
	gemmbody.nseg = 1
}
proc geometry() {				
	soma { L=8  diam=8 }			
	dend { L=100 diam=1 }     // L = 50/100 !!!
	gemmshaft { L=1 diam=1 }
	gemmbody  { L=1 diam=1 }
	define_shape()					// Fill in empty pt3d info with est's using 'L' and 'diam' info
}

proc biophysics() {
    
    parameter()
	spiketimes = new Vector()
	dendspike  = new Vector()
	
	spike_threshold = -10  // !!!
	
	forsec pg_all { 				// insert passive current everywhere
		Ra = 80         // OLD: 173
		cm = 1.2        // 2 !!!
		
		insert pas
		  g_pas = 1/RM 	// OLD: 2.3e-4 (4.3 K ohm*cm^2)
		  e_pas = EL		
	}
	
	soma {	
	
	insert nax
        gbar_nax  = gNa_Soma
        sh_nax  = Sh_Na	
	insert kdrmt
	    gbar_kdrmt = gKdr_Soma  
        q10_kdrmt  = 3		
	insert kamt
        gbar_kamt = gA_Soma    // 
		sha_kamt  = Sha_A 
		shi_kamt  = Shi_A
		k_tauH_kamt = k_tauH
		sh_tauH_kamt = sh_tauH
    insert kM
        gkbar_kM  = gM_Soma    // 	
   	
	insert Icapn
        gbar_Icapn = gCapn_Soma		
	insert Ikca
        gkbar_Ikca = gKCa_Soma
		
	insert Icat
	    gbar_Icat = gCaT_Soma 
		sha_Icat  = Sha_T
		shi_Icat  = Shi_T
	insert cad2     
        depth_cad2  = shell_depth
			
	ena = ENA
	ek  = EK
    eca = ECA
	
	spikecount  = new APCount(0.5)
    spikecount.thresh = spike_threshold
    spikecount.record(spiketimes)
	}
	
	forsec pgdendgemm {		
	insert nax
        gbar_nax = gNa_Dend	
		sh_nax  = Sh_Na
    insert kdrmt
	    gbar_kdrmt = gKdr_Dend   
        q10_kdrmt  = 3		
	insert kamt
        gbar_kamt  = gA_Dend    // 
		sha_kamt = Sha_A 
		shi_kamt = Shi_A
		k_tauH_kamt = k_tauH
		sh_tauH_kamt = sh_tauH		
	insert kM
        gkbar_kM  = gM_Dend  // 
	
	insert hpg 			// PG cell h current from Cadetti and Belluzzi
	  eh_hpg = 0 
	  ghbar_hpg = gH_Dend
	  
	insert Ican
	    gbar_Ican = gcan_Dend 
	insert Ikca
        gkbar_Ikca = gKCa_Dend 
	insert Icapn
        gbar_Icapn = gCapn_Dend	
 	    
	insert Icat
	    gbar_Icat = gCaT_Dend 
		sha_Icat  = Sha_T
		shi_Icat  = Shi_T
		k_tauH_Icat = K_tauH
	insert cad2    
        depth_cad2 = shell_depth		
	
	ena = ENA
	ek  = EK
	eca = ECA
	}
	
	forsec spine {	
	
	insert Inic
	enic_Inic = 3.2
	gbar_Inic = $1   // S/cm2   
	
	}
	
		
   gemmbody{
   
	AMPAr = new ExpSyn(0.5)
    AMPAr.tau = AMPAtau
    AMPAr.e 	= Erev
	  
	dendcount = new APCount(0.5)
    dendcount.thresh = spike_threshold
    dendcount.record(dendspike)
    }
	

}


proc init() {
  celldef($1)
}

endtemplate PGcell