This repository contains open-source code, data, & text files for the gulf stream microbiomes project
Project: Characterizing microbial community composition associated with Sargassum communities
Raw sequence files can be found at NCBI SRA BioProject ID ().
- Aim 1): Characterize patterns of microbial community composition (bacterioplankton, microeukaryotes) associated with pelagic Sargassum
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analyses:: An R Markdown file that includes R script written by Mario Muscarella and Ariane Peralta containing functions used in analysis of microbial sequence data.
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bin:
- MothurTools.R: An R script written by Mario Muscarella. R script contains functions used in the analysis of community sequence data.
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data:: Files associated with soil and microbial data sets.
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figures:: Figures (of zooplankton and bacterioplankton structure and function) generated according to R script located in R Markdown file.
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mothur:: Files containing script for bioinformatic analysis of 16S rRNA gene sequences (Illumina MiSeq platform) using mothur pipeline.
North Carolina Ocean Energy Program
Dr. Lindsay Dubbs (website): Principal Investigator, Research Assistant Professor Co-Director, Outer Banks Field Site, Institute for the Environment, University of North Carolina-Chapel Hill. Led and funded examination of pelagic Sargassum community primary productivity and nutrient cycling in the Gulf Stream.
Claire Johnson: Co-Investigator, Graduate Student in the Environment, Ecology, and Energy Program, University of North Carolina-Chapel Hill. Member of the Dubbs Lab. Institute for the Environment, UNC-Chapel Hill. Led and conducted field sampling and processing to study nutrient cycling and nitrogen fixation by the Sargassum community in the Gulf Stream.
Dr. Mario Muscarella: Assistant Professor, Institute or Arctic Biology, University of Alaska Fairbanks. Principal Investigator of the Muscarella Lab. Analyzed data and interpreted results.
Dr. Ariane Peralta (website): Co-Investigator. Associate Professor, Department of Biology, East Carolina University. Designed microbiome sampling and lab protocols, analyzed data, and interpreted results.