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Perennial grain crop, soil food web and soil carbon dynamics

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PWES_SoilBio

This repository contains open-source code, data, & text files for

For information regarding the project, please visit:

Sprunger, C., S.W. Culman, A.L. Peralta, S.T. DuPont, J.T. Lennon, and S.S. Snapp (2019) Perennial grain crop roots and nitrogen management shape soil food webs and soil carbon dynamics. Soil Biology and Biochemistry. 137. https://doi.org/10.1016/j.soilbio.2019.107573

Raw sequences can be found at NCBI SRA BioProject ID PRJNA548308.

We address the following questions

  • Aim 1.): How does perenniality and different N management influence roots, soil carbon pools, and soil food webs?

  • Aim 2.): In what ways could a perennial grain crop positively impact soil carbon stabilization and food web structure and function?

Repo Contents

  • analyses:: An R Markdown file that includes R script written by Ariane Peralta containing functions used in analysis of soil and microbial sequence data.

  • bin:

    • MothurTools.R: An R script written by Mario Muscarella (Indiana University, now at University of Illinois) containing functions used in the analysis of community sequence data.
  • data:: Files associated with soil and microbial data sets.

  • figures:: Figures (of soil, microbes) generated according to R script located in R Markdown file.

  • mothur:: Files containing script for bioinformatic analysis of 16S rRNA gene sequences (Illumina MiSeq platform) using mothur pipeline.

Contributors

Dr. Ariane Peralta: Assistant Professor, Department of Biology, East Carolina University. Principal Investigator of the Peralta Lab

Mario Muscarella: Postdoc in the O'Dwyer Lab at the University of Illinois at Urbana-Champaign

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