WRC15_Rhizo

This repository contains open-source code, data, & text files.

For information regarding the project, please visit: Bledsoe RB, Goodwillie C, and Peralta AL. 2020. Long-term nutrient enrichment of an oligotroph-dominated wetland increases bacterial diversity in bulk soils and plant rhizospheres. mSphere. 5(3). https://doi.org/10.1128/mSphere.00035-20

Raw sequence files can be found at NCBI SRA BioProject ID PRJNA599142.

We address the following question

• Aim: How does long-term nutrient additions impact microbial community diveristy, composition, and life-history in plant rhizospheres?

Repo Contents

• analyses: An R Markdown file that includes R script written by Regina Bledsoe, Ariane Peralta, and Mario Muscarella containing functions used in analysis of soil and microbial sequence data.

• bin:

  • MothurTools.R: An R script written by Mario Muscarella containing functions used in the analysis of community sequence data.

• data: Files associated with soil and microbial data sets.

• figures: Figures (of soil, microbes) generated according to R script located in R Markdown file.

• mothur: Files containing script for bioinformatic analysis of 16S rRNA gene sequences (Illumina MiSeq platform) using mothur pipeline.

Funding Sources

This work was supported by the East Carolina University and the National Science Foundation (GRFP to RBB, DEB 1845845 to ALP).

Contributors

Dr. Regina Bledsoe (website): Principal Investigator, PhD Student in the Peralta Lab at East Carolina University

Dr. Ariane Peralta (website): Co-Principal Investigator, Assistant Professor, Department of Biology, East Carolina University.

Dr. Carol Goodwillie (website): Co-Principal Investigator, Assistant Professor, Department of Biology, East Carolina University.

Dr. Mario Muscarella (website): Postdoc at Université du Quebéc à Montréal, soon to be Assistant Professor, University of Alaska Fairbanks