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I was looking at the --help page to adjust coverage but I got very confused as the flag for coverage is -a, which is also a flag for the mm2_options just above it and it can be interpreted as that the flags underneath the mm2_options belong to it instead of being separate. At least that is how I interpreted it and had a few problems (now fixed).
Alignments:
-mm2_options =STR space delimited minimap2 parameters. By default ="-a --end-bonus 5 --eqx -N 50 -p 0.5"
-a A designate a feature mapped only if it aligns with coverage ≥A; by default A=0.5
-s S designate a feature mapped only if its child features (usually exons/CDS) align with sequence identity ≥S; by default S=0.5
-d D distance scaling factor; alignment nodes separated by more than a factor of D in the target genome will not be connected in the
graph; by default D=2.0
-flank F amount of flanking sequence to align as a fraction [0.0-1.0] of gene length. This can improve gene alignment where gene structure
differs between target and reference; by default F=0.0
As a suggestion maybe it would be good to clarify that the following alignment flags are not part of the mm2_options.
Many thanks
The text was updated successfully, but these errors were encountered:
Hello,
I was looking at the --help page to adjust coverage but I got very confused as the flag for coverage is
-a
, which is also a flag for themm2_options
just above it and it can be interpreted as that the flags underneath themm2_options
belong to it instead of being separate. At least that is how I interpreted it and had a few problems (now fixed).As a suggestion maybe it would be good to clarify that the following alignment flags are not part of the
mm2_options
.Many thanks
The text was updated successfully, but these errors were encountered: