diff --git a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/IntegTestDefaultTestDeployAssertE3E7D2A4.assets.json b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/IntegTestDefaultTestDeployAssertE3E7D2A4.assets.json
index 2220abb680bef..87dfbae32bf67 100644
--- a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/IntegTestDefaultTestDeployAssertE3E7D2A4.assets.json
+++ b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/IntegTestDefaultTestDeployAssertE3E7D2A4.assets.json
@@ -1,5 +1,5 @@
 {
-  "version": "34.0.0",
+  "version": "36.0.0",
   "files": {
     "21fbb51d7b23f6a6c262b46a9caee79d744a3ac019fd45422d988b96d44b2a22": {
       "source": {
diff --git a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack1.assets.json b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack1.assets.json
index ab5cc895e502e..175b912c3170d 100644
--- a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack1.assets.json
+++ b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack1.assets.json
@@ -1,7 +1,7 @@
 {
-  "version": "34.0.0",
+  "version": "36.0.0",
   "files": {
-    "75e2bf084797f71f99ed5cb6f35bb6f1ee8a42cbbdb4ccd99008c5792941bbed": {
+    "7ef57b26c193be4a2756182b2cc3ab8a4116331e8195f1ac25001c2c6132cac8": {
       "source": {
         "path": "Stack1.template.json",
         "packaging": "file"
@@ -9,7 +9,7 @@
       "destinations": {
         "current_account-current_region": {
           "bucketName": "cdk-hnb659fds-assets-${AWS::AccountId}-${AWS::Region}",
-          "objectKey": "75e2bf084797f71f99ed5cb6f35bb6f1ee8a42cbbdb4ccd99008c5792941bbed.json",
+          "objectKey": "7ef57b26c193be4a2756182b2cc3ab8a4116331e8195f1ac25001c2c6132cac8.json",
           "assumeRoleArn": "arn:${AWS::Partition}:iam::${AWS::AccountId}:role/cdk-hnb659fds-file-publishing-role-${AWS::AccountId}-${AWS::Region}"
         }
       }
diff --git a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack1.template.json b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack1.template.json
index 4a1fc9fb25200..7353b8a8381a8 100644
--- a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack1.template.json
+++ b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack1.template.json
@@ -79,7 +79,7 @@
         {
          "Ref": "AWS::Region"
         },
-        ":901920570463:layer:aws-otel-nodejs-arm64-ver-1-17-1:1"
+        ":901920570463:layer:aws-otel-nodejs-arm64-ver-1-18-1:1"
        ]
       ]
      }
@@ -176,7 +176,7 @@
         {
          "Ref": "AWS::Region"
         },
-        ":901920570463:layer:aws-otel-python-amd64-ver-1-20-0:3"
+        ":901920570463:layer:aws-otel-python-amd64-ver-1-21-0:1"
        ]
       ]
      }
@@ -273,7 +273,7 @@
         {
          "Ref": "AWS::Region"
         },
-        ":901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-31-0:1"
+        ":901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-32-0:1"
        ]
       ]
      }
@@ -370,7 +370,7 @@
         {
          "Ref": "AWS::Region"
         },
-        ":901920570463:layer:aws-otel-java-agent-amd64-ver-1-31-0:1"
+        ":901920570463:layer:aws-otel-java-agent-amd64-ver-1-32-0:1"
        ]
       ]
      }
@@ -467,7 +467,7 @@
         {
          "Ref": "AWS::Region"
         },
-        ":901920570463:layer:aws-otel-collector-amd64-ver-0-88-0:1"
+        ":901920570463:layer:aws-otel-collector-amd64-ver-0-90-1:1"
        ]
       ]
      }
diff --git a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack2.assets.json b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack2.assets.json
index 51b95077e58b4..7d7413cceea49 100644
--- a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack2.assets.json
+++ b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack2.assets.json
@@ -1,7 +1,7 @@
 {
-  "version": "34.0.0",
+  "version": "36.0.0",
   "files": {
-    "0a53257b39135563c9bc8a0266ed700fb15d8858f13e44c476dbfd549668a4c6": {
+    "8854abef6af7d861552fb50928fa764ab1ff2a66641e5f4f0e02fdcc23300c53": {
       "source": {
         "path": "Stack2.template.json",
         "packaging": "file"
@@ -9,7 +9,7 @@
       "destinations": {
         "current_account-current_region": {
           "bucketName": "cdk-hnb659fds-assets-${AWS::AccountId}-${AWS::Region}",
-          "objectKey": "0a53257b39135563c9bc8a0266ed700fb15d8858f13e44c476dbfd549668a4c6.json",
+          "objectKey": "8854abef6af7d861552fb50928fa764ab1ff2a66641e5f4f0e02fdcc23300c53.json",
           "assumeRoleArn": "arn:${AWS::Partition}:iam::${AWS::AccountId}:role/cdk-hnb659fds-file-publishing-role-${AWS::AccountId}-${AWS::Region}"
         }
       }
diff --git a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack2.template.json b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack2.template.json
index 3a6b00dffb107..a1a03e1e2803b 100644
--- a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack2.template.json
+++ b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack2.template.json
@@ -79,7 +79,7 @@
         {
          "Ref": "AWS::Region"
         },
-        ":901920570463:layer:aws-otel-nodejs-amd64-ver-1-17-1:1"
+        ":901920570463:layer:aws-otel-nodejs-amd64-ver-1-18-1:1"
        ]
       ]
      }
@@ -176,7 +176,7 @@
         {
          "Ref": "AWS::Region"
         },
-        ":901920570463:layer:aws-otel-python-amd64-ver-1-20-0:3"
+        ":901920570463:layer:aws-otel-python-amd64-ver-1-21-0:1"
        ]
       ]
      }
@@ -273,7 +273,7 @@
         {
          "Ref": "AWS::Region"
         },
-        ":901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-31-0:1"
+        ":901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-32-0:1"
        ]
       ]
      }
@@ -370,7 +370,7 @@
         {
          "Ref": "AWS::Region"
         },
-        ":901920570463:layer:aws-otel-java-agent-amd64-ver-1-31-0:1"
+        ":901920570463:layer:aws-otel-java-agent-amd64-ver-1-32-0:1"
        ]
       ]
      }
@@ -467,7 +467,7 @@
         {
          "Ref": "AWS::Region"
         },
-        ":901920570463:layer:aws-otel-collector-amd64-ver-0-88-0:1"
+        ":901920570463:layer:aws-otel-collector-amd64-ver-0-90-1:1"
        ]
       ]
      }
diff --git a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/cdk.out b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/cdk.out
index 2313ab5436501..1f0068d32659a 100644
--- a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/cdk.out
+++ b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/cdk.out
@@ -1 +1 @@
-{"version":"34.0.0"}
\ No newline at end of file
+{"version":"36.0.0"}
\ No newline at end of file
diff --git a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/integ.json b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/integ.json
index 512238fe5f972..95df7ed211dc8 100644
--- a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/integ.json
+++ b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/integ.json
@@ -1,5 +1,5 @@
 {
-  "version": "34.0.0",
+  "version": "36.0.0",
   "testCases": {
     "IntegTest/DefaultTest": {
       "stacks": [
diff --git a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/manifest.json b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/manifest.json
index b586de7a08483..8e6c3ff76057f 100644
--- a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/manifest.json
+++ b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/manifest.json
@@ -1,5 +1,5 @@
 {
-  "version": "34.0.0",
+  "version": "36.0.0",
   "artifacts": {
     "Stack1.assets": {
       "type": "cdk:asset-manifest",
@@ -18,7 +18,7 @@
         "validateOnSynth": false,
         "assumeRoleArn": "arn:${AWS::Partition}:iam::${AWS::AccountId}:role/cdk-hnb659fds-deploy-role-${AWS::AccountId}-${AWS::Region}",
         "cloudFormationExecutionRoleArn": "arn:${AWS::Partition}:iam::${AWS::AccountId}:role/cdk-hnb659fds-cfn-exec-role-${AWS::AccountId}-${AWS::Region}",
-        "stackTemplateAssetObjectUrl": "s3://cdk-hnb659fds-assets-${AWS::AccountId}-${AWS::Region}/75e2bf084797f71f99ed5cb6f35bb6f1ee8a42cbbdb4ccd99008c5792941bbed.json",
+        "stackTemplateAssetObjectUrl": "s3://cdk-hnb659fds-assets-${AWS::AccountId}-${AWS::Region}/7ef57b26c193be4a2756182b2cc3ab8a4116331e8195f1ac25001c2c6132cac8.json",
         "requiresBootstrapStackVersion": 6,
         "bootstrapStackVersionSsmParameter": "/cdk-bootstrap/hnb659fds/version",
         "additionalDependencies": [
@@ -156,7 +156,7 @@
         "validateOnSynth": false,
         "assumeRoleArn": "arn:${AWS::Partition}:iam::${AWS::AccountId}:role/cdk-hnb659fds-deploy-role-${AWS::AccountId}-${AWS::Region}",
         "cloudFormationExecutionRoleArn": "arn:${AWS::Partition}:iam::${AWS::AccountId}:role/cdk-hnb659fds-cfn-exec-role-${AWS::AccountId}-${AWS::Region}",
-        "stackTemplateAssetObjectUrl": "s3://cdk-hnb659fds-assets-${AWS::AccountId}-${AWS::Region}/0a53257b39135563c9bc8a0266ed700fb15d8858f13e44c476dbfd549668a4c6.json",
+        "stackTemplateAssetObjectUrl": "s3://cdk-hnb659fds-assets-${AWS::AccountId}-${AWS::Region}/8854abef6af7d861552fb50928fa764ab1ff2a66641e5f4f0e02fdcc23300c53.json",
         "requiresBootstrapStackVersion": 6,
         "bootstrapStackVersionSsmParameter": "/cdk-bootstrap/hnb659fds/version",
         "additionalDependencies": [
diff --git a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/tree.json b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/tree.json
index 62bd8473a9778..62b57c2cc016a 100644
--- a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/tree.json
+++ b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/tree.json
@@ -138,7 +138,7 @@
                             {
                               "Ref": "AWS::Region"
                             },
-                            ":901920570463:layer:aws-otel-nodejs-arm64-ver-1-17-1:1"
+                            ":901920570463:layer:aws-otel-nodejs-arm64-ver-1-18-1:1"
                           ]
                         ]
                       }
@@ -301,7 +301,7 @@
                             {
                               "Ref": "AWS::Region"
                             },
-                            ":901920570463:layer:aws-otel-python-amd64-ver-1-20-0:3"
+                            ":901920570463:layer:aws-otel-python-amd64-ver-1-21-0:1"
                           ]
                         ]
                       }
@@ -464,7 +464,7 @@
                             {
                               "Ref": "AWS::Region"
                             },
-                            ":901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-31-0:1"
+                            ":901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-32-0:1"
                           ]
                         ]
                       }
@@ -627,7 +627,7 @@
                             {
                               "Ref": "AWS::Region"
                             },
-                            ":901920570463:layer:aws-otel-java-agent-amd64-ver-1-31-0:1"
+                            ":901920570463:layer:aws-otel-java-agent-amd64-ver-1-32-0:1"
                           ]
                         ]
                       }
@@ -790,7 +790,7 @@
                             {
                               "Ref": "AWS::Region"
                             },
-                            ":901920570463:layer:aws-otel-collector-amd64-ver-0-88-0:1"
+                            ":901920570463:layer:aws-otel-collector-amd64-ver-0-90-1:1"
                           ]
                         ]
                       }
@@ -982,7 +982,7 @@
                             {
                               "Ref": "AWS::Region"
                             },
-                            ":901920570463:layer:aws-otel-nodejs-amd64-ver-1-17-1:1"
+                            ":901920570463:layer:aws-otel-nodejs-amd64-ver-1-18-1:1"
                           ]
                         ]
                       }
@@ -1145,7 +1145,7 @@
                             {
                               "Ref": "AWS::Region"
                             },
-                            ":901920570463:layer:aws-otel-python-amd64-ver-1-20-0:3"
+                            ":901920570463:layer:aws-otel-python-amd64-ver-1-21-0:1"
                           ]
                         ]
                       }
@@ -1308,7 +1308,7 @@
                             {
                               "Ref": "AWS::Region"
                             },
-                            ":901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-31-0:1"
+                            ":901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-32-0:1"
                           ]
                         ]
                       }
@@ -1471,7 +1471,7 @@
                             {
                               "Ref": "AWS::Region"
                             },
-                            ":901920570463:layer:aws-otel-java-agent-amd64-ver-1-31-0:1"
+                            ":901920570463:layer:aws-otel-java-agent-amd64-ver-1-32-0:1"
                           ]
                         ]
                       }
@@ -1634,7 +1634,7 @@
                             {
                               "Ref": "AWS::Region"
                             },
-                            ":901920570463:layer:aws-otel-collector-amd64-ver-0-88-0:1"
+                            ":901920570463:layer:aws-otel-collector-amd64-ver-0-90-1:1"
                           ]
                         ]
                       }
@@ -1705,7 +1705,7 @@
                 "path": "IntegTest/DefaultTest/Default",
                 "constructInfo": {
                   "fqn": "constructs.Construct",
-                  "version": "10.2.70"
+                  "version": "10.3.0"
                 }
               },
               "DeployAssert": {
@@ -1751,7 +1751,7 @@
         "path": "Tree",
         "constructInfo": {
           "fqn": "constructs.Construct",
-          "version": "10.2.70"
+          "version": "10.3.0"
         }
       }
     },
diff --git a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.ts b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.ts
index fa3b24b68a30a..dbde2da139a23 100644
--- a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.ts
+++ b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.ts
@@ -33,7 +33,7 @@ class StackUnderTest extends Stack {
       code: Code.fromInline(`exports.handler = ${handler.toString()}`),
       architecture: props.architecture,
       adotInstrumentation: {
-        layerVersion: AdotLayerVersion.fromJavaScriptSdkLayerVersion(AdotLambdaLayerJavaScriptSdkVersion.V1_17_1),
+        layerVersion: AdotLayerVersion.fromJavaScriptSdkLayerVersion(AdotLambdaLayerJavaScriptSdkVersion.LATEST),
         execWrapper: AdotLambdaExecWrapper.REGULAR_HANDLER,
       },
     });
@@ -43,7 +43,7 @@ class StackUnderTest extends Stack {
       handler: 'index.handler',
       code: Code.fromInline('def handler(event, context): pass'),
       adotInstrumentation: {
-        layerVersion: AdotLayerVersion.fromPythonSdkLayerVersion(AdotLambdaLayerPythonSdkVersion.V1_20_0_1),
+        layerVersion: AdotLayerVersion.fromPythonSdkLayerVersion(AdotLambdaLayerPythonSdkVersion.LATEST),
         execWrapper: AdotLambdaExecWrapper.INSTRUMENT_HANDLER,
       },
     });
@@ -53,7 +53,7 @@ class StackUnderTest extends Stack {
       handler: 'index.handler',
       code: Code.fromInline('def handler(event, context): pass'),
       adotInstrumentation: {
-        layerVersion: AdotLayerVersion.fromJavaSdkLayerVersion(AdotLambdaLayerJavaSdkVersion.V1_31_0),
+        layerVersion: AdotLayerVersion.fromJavaSdkLayerVersion(AdotLambdaLayerJavaSdkVersion.LATEST),
         execWrapper: AdotLambdaExecWrapper.INSTRUMENT_HANDLER,
       },
     });
@@ -63,7 +63,7 @@ class StackUnderTest extends Stack {
       handler: 'index.handler',
       code: Code.fromInline('def handler(event, context): pass'),
       adotInstrumentation: {
-        layerVersion: AdotLayerVersion.fromJavaAutoInstrumentationLayerVersion(AdotLambdaLayerJavaAutoInstrumentationVersion.V1_31_0),
+        layerVersion: AdotLayerVersion.fromJavaAutoInstrumentationLayerVersion(AdotLambdaLayerJavaAutoInstrumentationVersion.LATEST),
         execWrapper: AdotLambdaExecWrapper.INSTRUMENT_HANDLER,
       },
     });
@@ -73,7 +73,7 @@ class StackUnderTest extends Stack {
       handler: 'index.handler',
       code: Code.fromInline('def handler(event, context): pass'),
       adotInstrumentation: {
-        layerVersion: AdotLayerVersion.fromGenericLayerVersion(AdotLambdaLayerGenericVersion.V0_88_0),
+        layerVersion: AdotLayerVersion.fromGenericLayerVersion(AdotLambdaLayerGenericVersion.LATEST),
         execWrapper: AdotLambdaExecWrapper.INSTRUMENT_HANDLER,
       },
     });
diff --git a/packages/aws-cdk-lib/aws-lambda/lib/adot-layers.ts b/packages/aws-cdk-lib/aws-lambda/lib/adot-layers.ts
index 7b7742672fcc2..a6bd12db8f5b8 100644
--- a/packages/aws-cdk-lib/aws-lambda/lib/adot-layers.ts
+++ b/packages/aws-cdk-lib/aws-lambda/lib/adot-layers.ts
@@ -209,6 +209,11 @@ abstract class AdotLambdaLayerVersion {
  * The collection of versions of the ADOT Lambda Layer for Java SDK
  */
 export class AdotLambdaLayerJavaSdkVersion extends AdotLambdaLayerVersion {
+  /**
+   * Version 1.32.0
+   */
+  public static readonly V1_32_0 = new AdotLambdaLayerJavaSdkVersion('1.32.0');
+
   /**
    * Version 1.31.0
    */
@@ -233,7 +238,7 @@ export class AdotLambdaLayerJavaSdkVersion extends AdotLambdaLayerVersion {
    * The latest layer version available in this CDK version. New versions could
    * introduce incompatible changes. Make sure to test them before deploying to production.
    */
-  public static readonly LATEST = this.V1_31_0;
+  public static readonly LATEST = this.V1_32_0;
 
   private constructor(protected readonly layerVersion: string) {
     super(AdotLambdaLayerType.JAVA_SDK, layerVersion);
@@ -244,6 +249,11 @@ export class AdotLambdaLayerJavaSdkVersion extends AdotLambdaLayerVersion {
  * The collection of versions of the ADOT Lambda Layer for Java auto-instrumentation
  */
 export class AdotLambdaLayerJavaAutoInstrumentationVersion extends AdotLambdaLayerVersion {
+  /**
+   * Version 1.32.0
+   */
+  public static readonly V1_32_0 = new AdotLambdaLayerJavaAutoInstrumentationVersion('1.32.0');
+
   /**
    * Version 1.31.0
    */
@@ -268,7 +278,7 @@ export class AdotLambdaLayerJavaAutoInstrumentationVersion extends AdotLambdaLay
    * The latest layer version available in this CDK version. New versions could
    * introduce incompatible changes. Make sure to test them before deploying to production.
    */
-  public static readonly LATEST = this.V1_31_0;
+  public static readonly LATEST = this.V1_32_0;
 
   private constructor(protected readonly layerVersion: string) {
     super(AdotLambdaLayerType.JAVA_AUTO_INSTRUMENTATION, layerVersion);
@@ -279,6 +289,11 @@ export class AdotLambdaLayerJavaAutoInstrumentationVersion extends AdotLambdaLay
  * The collection of versions of the ADOT Lambda Layer for Python SDK
  */
 export class AdotLambdaLayerPythonSdkVersion extends AdotLambdaLayerVersion {
+  /**
+   * Version 1.21.0
+   */
+  public static readonly V1_21_0 = new AdotLambdaLayerPythonSdkVersion('1.21.0');
+
   /**
    * Version 1.20.0
    */
@@ -328,7 +343,7 @@ export class AdotLambdaLayerPythonSdkVersion extends AdotLambdaLayerVersion {
    * The latest layer version available in this CDK version. New versions could
    * introduce incompatible changes. Make sure to test them before deploying to production.
    */
-  public static readonly LATEST = this.V1_20_0_1;
+  public static readonly LATEST = this.V1_21_0;
 
   private constructor(protected readonly layerVersion: string) {
     super(AdotLambdaLayerType.PYTHON_SDK, layerVersion);
@@ -339,6 +354,11 @@ export class AdotLambdaLayerPythonSdkVersion extends AdotLambdaLayerVersion {
  * The collection of versions of the ADOT Lambda Layer for JavaScript SDK
  */
 export class AdotLambdaLayerJavaScriptSdkVersion extends AdotLambdaLayerVersion {
+  /**
+   * Version 1.18.1
+   */
+  public static readonly V1_18_1 = new AdotLambdaLayerJavaScriptSdkVersion('1.18.1');
+
   /**
    * Version 1.17.1
    */
@@ -363,7 +383,7 @@ export class AdotLambdaLayerJavaScriptSdkVersion extends AdotLambdaLayerVersion
    * The latest layer version available in this CDK version. New versions could
    * introduce incompatible changes. Make sure to test them before deploying to production.
    */
-  public static readonly LATEST = this.V1_17_1;
+  public static readonly LATEST = this.V1_18_1;
 
   private constructor(protected readonly layerVersion: string) {
     super(AdotLambdaLayerType.JAVASCRIPT_SDK, layerVersion);
@@ -374,6 +394,11 @@ export class AdotLambdaLayerJavaScriptSdkVersion extends AdotLambdaLayerVersion
  * The collection of versions of the ADOT Lambda Layer for generic purpose
  */
 export class AdotLambdaLayerGenericVersion extends AdotLambdaLayerVersion {
+  /**
+   * Version 0.90.1
+   */
+  public static readonly V0_90_1 = new AdotLambdaLayerGenericVersion('0.90.1');
+
   /**
    * Version 0.88.0
    */
@@ -398,7 +423,8 @@ export class AdotLambdaLayerGenericVersion extends AdotLambdaLayerVersion {
    * The latest layer version available in this CDK version. New versions could
    * introduce incompatible changes. Make sure to test them before deploying to production.
    */
-  public static readonly LATEST = this.V0_88_0;
+  public static readonly LATEST = this.V0_90_1;
+
   private constructor(protected readonly layerVersion: string) {
     super(AdotLambdaLayerType.GENERIC, layerVersion);
   }
diff --git a/packages/aws-cdk-lib/aws-lambda/test/adot.test.ts b/packages/aws-cdk-lib/aws-lambda/test/adot.test.ts
index 4ae124612f2d6..7a19227033240 100644
--- a/packages/aws-cdk-lib/aws-lambda/test/adot.test.ts
+++ b/packages/aws-cdk-lib/aws-lambda/test/adot.test.ts
@@ -21,10 +21,10 @@ describe('ADOT Lambda Layer', () => {
     });
 
     test('is added properly when the region information is available at synthesis time', () => {
-      const layerArn = lambda.AdotLambdaLayerJavaSdkVersion.V1_31_0.layerArn(fn.stack, fn.architecture);
+      const layerArn = lambda.AdotLambdaLayerJavaSdkVersion.V1_32_0.layerArn(fn.stack, fn.architecture);
 
       expect(layerArn).toEqual(
-        'arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-31-0:1',
+        'arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-32-0:1',
       );
     });
 
@@ -32,7 +32,7 @@ describe('ADOT Lambda Layer', () => {
       const layerArn = lambda.AdotLambdaLayerJavaSdkVersion.LATEST.layerArn(fn.stack, fn.architecture);
 
       expect(layerArn).toEqual(
-        'arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-31-0:1',
+        'arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-32-0:1',
       );
     });
   });
@@ -53,10 +53,10 @@ describe('ADOT Lambda Layer', () => {
     });
 
     test('is added properly when the region information is available at synthesis time', () => {
-      const layerArn = lambda.AdotLambdaLayerPythonSdkVersion.V1_20_0_1.layerArn(fn.stack, fn.architecture);
+      const layerArn = lambda.AdotLambdaLayerPythonSdkVersion.V1_21_0.layerArn(fn.stack, fn.architecture);
 
       expect(layerArn).toEqual(
-        'arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-python-amd64-ver-1-20-0:3',
+        'arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-python-amd64-ver-1-21-0:1',
       );
     });
 
@@ -64,7 +64,7 @@ describe('ADOT Lambda Layer', () => {
       const layerArn = lambda.AdotLambdaLayerPythonSdkVersion.LATEST.layerArn(fn.stack, fn.architecture);
 
       expect(layerArn).toEqual(
-        'arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-python-amd64-ver-1-20-0:3',
+        'arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-python-amd64-ver-1-21-0:1',
       );
     });
   });
@@ -114,7 +114,7 @@ describe('ADOT Lambda Layer', () => {
               {
                 Ref: 'AWS::Region',
               },
-              ':901920570463:layer:aws-otel-java-wrapper-arm64-ver-1-31-0:1',
+              ':901920570463:layer:aws-otel-java-wrapper-arm64-ver-1-32-0:1',
             ],
           ],
         },
diff --git a/packages/aws-cdk-lib/aws-lambda/test/function.test.ts b/packages/aws-cdk-lib/aws-lambda/test/function.test.ts
index 03bd96357a992..4d4984df28b05 100644
--- a/packages/aws-cdk-lib/aws-lambda/test/function.test.ts
+++ b/packages/aws-cdk-lib/aws-lambda/test/function.test.ts
@@ -3313,14 +3313,14 @@ describe('function', () => {
       handler: 'index.handler',
       runtime: lambda.Runtime.NODEJS_LATEST,
       adotInstrumentation: {
-        layerVersion: lambda.AdotLayerVersion.fromJavaSdkLayerVersion(AdotLambdaLayerJavaSdkVersion.V1_31_0),
+        layerVersion: lambda.AdotLayerVersion.fromJavaSdkLayerVersion(AdotLambdaLayerJavaSdkVersion.V1_32_0),
         execWrapper: lambda.AdotLambdaExecWrapper.REGULAR_HANDLER,
       },
     });
 
     // THEN
     Template.fromStack(stack).hasResourceProperties('AWS::Lambda::Function', {
-      Layers: ['arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-31-0:1'],
+      Layers: ['arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-32-0:1'],
       Environment: {
         Variables: {
           AWS_LAMBDA_EXEC_WRAPPER: '/opt/otel-handler',
@@ -3342,14 +3342,14 @@ describe('function', () => {
       handler: 'index.handler',
       runtime: lambda.Runtime.PYTHON_3_9,
       adotInstrumentation: {
-        layerVersion: lambda.AdotLayerVersion.fromPythonSdkLayerVersion(lambda.AdotLambdaLayerPythonSdkVersion.V1_20_0_1),
+        layerVersion: lambda.AdotLayerVersion.fromPythonSdkLayerVersion(lambda.AdotLambdaLayerPythonSdkVersion.V1_21_0),
         execWrapper: lambda.AdotLambdaExecWrapper.INSTRUMENT_HANDLER,
       },
     });
 
     // THEN
     Template.fromStack(stack).hasResourceProperties('AWS::Lambda::Function', {
-      Layers: ['arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-python-amd64-ver-1-20-0:3'],
+      Layers: ['arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-python-amd64-ver-1-21-0:1'],
       Environment: {
         Variables: {
           AWS_LAMBDA_EXEC_WRAPPER: '/opt/otel-instrument',
@@ -3366,7 +3366,7 @@ describe('function', () => {
       handler: 'index.handler',
       runtime: lambda.Runtime.PYTHON_3_10,
       adotInstrumentation: {
-        layerVersion: lambda.AdotLayerVersion.fromPythonSdkLayerVersion(lambda.AdotLambdaLayerPythonSdkVersion.V1_20_0_1),
+        layerVersion: lambda.AdotLayerVersion.fromPythonSdkLayerVersion(lambda.AdotLambdaLayerPythonSdkVersion.V1_21_0),
         execWrapper: lambda.AdotLambdaExecWrapper.REGULAR_HANDLER,
       },
     })).toThrow(/Python Adot Lambda layer requires AdotLambdaExecWrapper.INSTRUMENT_HANDLER/);
@@ -3385,7 +3385,7 @@ describe('function', () => {
         new lambda.DockerImageFunction(stack, 'MyLambda', {
           code: lambda.DockerImageCode.fromImageAsset(dockerLambdaHandlerPath),
           adotInstrumentation: {
-            layerVersion: lambda.AdotLayerVersion.fromJavaSdkLayerVersion(AdotLambdaLayerJavaSdkVersion.V1_31_0),
+            layerVersion: lambda.AdotLayerVersion.fromJavaSdkLayerVersion(AdotLambdaLayerJavaSdkVersion.V1_32_0),
             execWrapper: lambda.AdotLambdaExecWrapper.REGULAR_HANDLER,
           },
         }),
diff --git a/packages/aws-cdk-lib/region-info/build-tools/fact-tables.ts b/packages/aws-cdk-lib/region-info/build-tools/fact-tables.ts
index b19b691f7a154..4284ceffa7931 100644
--- a/packages/aws-cdk-lib/region-info/build-tools/fact-tables.ts
+++ b/packages/aws-cdk-lib/region-info/build-tools/fact-tables.ts
@@ -825,6 +825,44 @@ export const PARAMS_AND_SECRETS_LAMBDA_LAYER_ARNS: { [version: string]: { [arch:
 };
 
 const ADOT_LAMBDA_LAYER_JAVA_SDK_ARNS: { [version: string]: { [arch: string]: { [region: string]: string } } } = {
+  '1.32.0': {
+    x86_64: {
+      'ap-northeast-1': 'arn:aws:lambda:ap-northeast-1:901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-32-0:1',
+      'ap-northeast-2': 'arn:aws:lambda:ap-northeast-2:901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-32-0:1',
+      'ap-south-1': 'arn:aws:lambda:ap-south-1:901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-32-0:1',
+      'ap-southeast-1': 'arn:aws:lambda:ap-southeast-1:901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-32-0:1',
+      'ap-southeast-2': 'arn:aws:lambda:ap-southeast-2:901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-32-0:1',
+      'ca-central-1': 'arn:aws:lambda:ca-central-1:901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-32-0:1',
+      'eu-central-1': 'arn:aws:lambda:eu-central-1:901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-32-0:1',
+      'eu-north-1': 'arn:aws:lambda:eu-north-1:901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-32-0:1',
+      'eu-west-1': 'arn:aws:lambda:eu-west-1:901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-32-0:1',
+      'eu-west-2': 'arn:aws:lambda:eu-west-2:901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-32-0:1',
+      'eu-west-3': 'arn:aws:lambda:eu-west-3:901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-32-0:1',
+      'sa-east-1': 'arn:aws:lambda:sa-east-1:901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-32-0:1',
+      'us-east-1': 'arn:aws:lambda:us-east-1:901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-32-0:1',
+      'us-east-2': 'arn:aws:lambda:us-east-2:901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-32-0:1',
+      'us-west-1': 'arn:aws:lambda:us-west-1:901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-32-0:1',
+      'us-west-2': 'arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-32-0:1',
+    },
+    arm64: {
+      'ap-northeast-1': 'arn:aws:lambda:ap-northeast-1:901920570463:layer:aws-otel-java-wrapper-arm64-ver-1-32-0:1',
+      'ap-northeast-2': 'arn:aws:lambda:ap-northeast-2:901920570463:layer:aws-otel-java-wrapper-arm64-ver-1-32-0:1',
+      'ap-south-1': 'arn:aws:lambda:ap-south-1:901920570463:layer:aws-otel-java-wrapper-arm64-ver-1-32-0:1',
+      'ap-southeast-1': 'arn:aws:lambda:ap-southeast-1:901920570463:layer:aws-otel-java-wrapper-arm64-ver-1-32-0:1',
+      'ap-southeast-2': 'arn:aws:lambda:ap-southeast-2:901920570463:layer:aws-otel-java-wrapper-arm64-ver-1-32-0:1',
+      'ca-central-1': 'arn:aws:lambda:ca-central-1:901920570463:layer:aws-otel-java-wrapper-arm64-ver-1-32-0:1',
+      'eu-central-1': 'arn:aws:lambda:eu-central-1:901920570463:layer:aws-otel-java-wrapper-arm64-ver-1-32-0:1',
+      'eu-north-1': 'arn:aws:lambda:eu-north-1:901920570463:layer:aws-otel-java-wrapper-arm64-ver-1-32-0:1',
+      'eu-west-1': 'arn:aws:lambda:eu-west-1:901920570463:layer:aws-otel-java-wrapper-arm64-ver-1-32-0:1',
+      'eu-west-2': 'arn:aws:lambda:eu-west-2:901920570463:layer:aws-otel-java-wrapper-arm64-ver-1-32-0:1',
+      'eu-west-3': 'arn:aws:lambda:eu-west-3:901920570463:layer:aws-otel-java-wrapper-arm64-ver-1-32-0:1',
+      'sa-east-1': 'arn:aws:lambda:sa-east-1:901920570463:layer:aws-otel-java-wrapper-arm64-ver-1-32-0:1',
+      'us-east-1': 'arn:aws:lambda:us-east-1:901920570463:layer:aws-otel-java-wrapper-arm64-ver-1-32-0:1',
+      'us-east-2': 'arn:aws:lambda:us-east-2:901920570463:layer:aws-otel-java-wrapper-arm64-ver-1-32-0:1',
+      'us-west-1': 'arn:aws:lambda:us-west-1:901920570463:layer:aws-otel-java-wrapper-arm64-ver-1-32-0:1',
+      'us-west-2': 'arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-java-wrapper-arm64-ver-1-32-0:1',
+    },
+  },
   '1.31.0': {
     x86_64: {
       // 'af-south-1': 'arn:aws:lambda:af-south-1:901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-31-0:1',
@@ -1310,6 +1348,44 @@ const ADOT_LAMBDA_LAYER_JAVA_SDK_ARNS: { [version: string]: { [arch: string]: {
 const ADOT_LAMBDA_LAYER_JAVA_AUTO_INSTRUMENTATION_ARNS: {
   [version: string]: { [arch: string]: { [region: string]: string } };
 } = {
+  '1.32.0': {
+    x86_64: {
+      'ap-northeast-1': 'arn:aws:lambda:ap-northeast-1:901920570463:layer:aws-otel-java-agent-amd64-ver-1-32-0:1',
+      'ap-northeast-2': 'arn:aws:lambda:ap-northeast-2:901920570463:layer:aws-otel-java-agent-amd64-ver-1-32-0:1',
+      'ap-south-1': 'arn:aws:lambda:ap-south-1:901920570463:layer:aws-otel-java-agent-amd64-ver-1-32-0:1',
+      'ap-southeast-1': 'arn:aws:lambda:ap-southeast-1:901920570463:layer:aws-otel-java-agent-amd64-ver-1-32-0:1',
+      'ap-southeast-2': 'arn:aws:lambda:ap-southeast-2:901920570463:layer:aws-otel-java-agent-amd64-ver-1-32-0:1',
+      'ca-central-1': 'arn:aws:lambda:ca-central-1:901920570463:layer:aws-otel-java-agent-amd64-ver-1-32-0:1',
+      'eu-central-1': 'arn:aws:lambda:eu-central-1:901920570463:layer:aws-otel-java-agent-amd64-ver-1-32-0:1',
+      'eu-north-1': 'arn:aws:lambda:eu-north-1:901920570463:layer:aws-otel-java-agent-amd64-ver-1-32-0:1',
+      'eu-west-1': 'arn:aws:lambda:eu-west-1:901920570463:layer:aws-otel-java-agent-amd64-ver-1-32-0:1',
+      'eu-west-2': 'arn:aws:lambda:eu-west-2:901920570463:layer:aws-otel-java-agent-amd64-ver-1-32-0:1',
+      'eu-west-3': 'arn:aws:lambda:eu-west-3:901920570463:layer:aws-otel-java-agent-amd64-ver-1-32-0:1',
+      'sa-east-1': 'arn:aws:lambda:sa-east-1:901920570463:layer:aws-otel-java-agent-amd64-ver-1-32-0:1',
+      'us-east-1': 'arn:aws:lambda:us-east-1:901920570463:layer:aws-otel-java-agent-amd64-ver-1-32-0:1',
+      'us-east-2': 'arn:aws:lambda:us-east-2:901920570463:layer:aws-otel-java-agent-amd64-ver-1-32-0:1',
+      'us-west-1': 'arn:aws:lambda:us-west-1:901920570463:layer:aws-otel-java-agent-amd64-ver-1-32-0:1',
+      'us-west-2': 'arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-java-agent-amd64-ver-1-32-0:1',
+    },
+    arm64: {
+      'ap-northeast-1': 'arn:aws:lambda:ap-northeast-1:901920570463:layer:aws-otel-java-agent-arm64-ver-1-32-0:1',
+      'ap-northeast-2': 'arn:aws:lambda:ap-northeast-2:901920570463:layer:aws-otel-java-agent-arm64-ver-1-32-0:1',
+      'ap-south-1': 'arn:aws:lambda:ap-south-1:901920570463:layer:aws-otel-java-agent-arm64-ver-1-32-0:1',
+      'ap-southeast-1': 'arn:aws:lambda:ap-southeast-1:901920570463:layer:aws-otel-java-agent-arm64-ver-1-32-0:1',
+      'ap-southeast-2': 'arn:aws:lambda:ap-southeast-2:901920570463:layer:aws-otel-java-agent-arm64-ver-1-32-0:1',
+      'ca-central-1': 'arn:aws:lambda:ca-central-1:901920570463:layer:aws-otel-java-agent-arm64-ver-1-32-0:1',
+      'eu-central-1': 'arn:aws:lambda:eu-central-1:901920570463:layer:aws-otel-java-agent-arm64-ver-1-32-0:1',
+      'eu-north-1': 'arn:aws:lambda:eu-north-1:901920570463:layer:aws-otel-java-agent-arm64-ver-1-32-0:1',
+      'eu-west-1': 'arn:aws:lambda:eu-west-1:901920570463:layer:aws-otel-java-agent-arm64-ver-1-32-0:1',
+      'eu-west-2': 'arn:aws:lambda:eu-west-2:901920570463:layer:aws-otel-java-agent-arm64-ver-1-32-0:1',
+      'eu-west-3': 'arn:aws:lambda:eu-west-3:901920570463:layer:aws-otel-java-agent-arm64-ver-1-32-0:1',
+      'sa-east-1': 'arn:aws:lambda:sa-east-1:901920570463:layer:aws-otel-java-agent-arm64-ver-1-32-0:1',
+      'us-east-1': 'arn:aws:lambda:us-east-1:901920570463:layer:aws-otel-java-agent-arm64-ver-1-32-0:1',
+      'us-east-2': 'arn:aws:lambda:us-east-2:901920570463:layer:aws-otel-java-agent-arm64-ver-1-32-0:1',
+      'us-west-1': 'arn:aws:lambda:us-west-1:901920570463:layer:aws-otel-java-agent-arm64-ver-1-32-0:1',
+      'us-west-2': 'arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-java-agent-arm64-ver-1-32-0:1',
+    },
+  },
   '1.31.0': {
     x86_64: {
       // 'af-south-1': 'arn:aws:lambda:af-south-1:901920570463:layer:aws-otel-java-agent-amd64-ver-1-31-0:1',
@@ -1792,6 +1868,44 @@ const ADOT_LAMBDA_LAYER_JAVA_AUTO_INSTRUMENTATION_ARNS: {
   },
 };
 const ADOT_LAMBDA_LAYER_JAVASCRIPT_SDK_ARNS: { [version: string]: { [arch: string]: { [region: string]: string } } } = {
+  '1.18.1': {
+    x86_64: {
+      'ap-northeast-1': 'arn:aws:lambda:ap-northeast-1:901920570463:layer:aws-otel-nodejs-amd64-ver-1-18-1:1',
+      'ap-northeast-2': 'arn:aws:lambda:ap-northeast-2:901920570463:layer:aws-otel-nodejs-amd64-ver-1-18-1:1',
+      'ap-south-1': 'arn:aws:lambda:ap-south-1:901920570463:layer:aws-otel-nodejs-amd64-ver-1-18-1:1',
+      'ap-southeast-1': 'arn:aws:lambda:ap-southeast-1:901920570463:layer:aws-otel-nodejs-amd64-ver-1-18-1:1',
+      'ap-southeast-2': 'arn:aws:lambda:ap-southeast-2:901920570463:layer:aws-otel-nodejs-amd64-ver-1-18-1:1',
+      'ca-central-1': 'arn:aws:lambda:ca-central-1:901920570463:layer:aws-otel-nodejs-amd64-ver-1-18-1:1',
+      'eu-central-1': 'arn:aws:lambda:eu-central-1:901920570463:layer:aws-otel-nodejs-amd64-ver-1-18-1:1',
+      'eu-north-1': 'arn:aws:lambda:eu-north-1:901920570463:layer:aws-otel-nodejs-amd64-ver-1-18-1:1',
+      'eu-west-1': 'arn:aws:lambda:eu-west-1:901920570463:layer:aws-otel-nodejs-amd64-ver-1-18-1:1',
+      'eu-west-2': 'arn:aws:lambda:eu-west-2:901920570463:layer:aws-otel-nodejs-amd64-ver-1-18-1:1',
+      'eu-west-3': 'arn:aws:lambda:eu-west-3:901920570463:layer:aws-otel-nodejs-amd64-ver-1-18-1:1',
+      'sa-east-1': 'arn:aws:lambda:sa-east-1:901920570463:layer:aws-otel-nodejs-amd64-ver-1-18-1:1',
+      'us-east-1': 'arn:aws:lambda:us-east-1:901920570463:layer:aws-otel-nodejs-amd64-ver-1-18-1:1',
+      'us-east-2': 'arn:aws:lambda:us-east-2:901920570463:layer:aws-otel-nodejs-amd64-ver-1-18-1:1',
+      'us-west-1': 'arn:aws:lambda:us-west-1:901920570463:layer:aws-otel-nodejs-amd64-ver-1-18-1:1',
+      'us-west-2': 'arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-nodejs-amd64-ver-1-18-1:1',
+    },
+    arm64: {
+      'ap-northeast-1': 'arn:aws:lambda:ap-northeast-1:901920570463:layer:aws-otel-nodejs-arm64-ver-1-18-1:1',
+      'ap-northeast-2': 'arn:aws:lambda:ap-northeast-2:901920570463:layer:aws-otel-nodejs-arm64-ver-1-18-1:1',
+      'ap-south-1': 'arn:aws:lambda:ap-south-1:901920570463:layer:aws-otel-nodejs-arm64-ver-1-18-1:1',
+      'ap-southeast-1': 'arn:aws:lambda:ap-southeast-1:901920570463:layer:aws-otel-nodejs-arm64-ver-1-18-1:1',
+      'ap-southeast-2': 'arn:aws:lambda:ap-southeast-2:901920570463:layer:aws-otel-nodejs-arm64-ver-1-18-1:1',
+      'ca-central-1': 'arn:aws:lambda:ca-central-1:901920570463:layer:aws-otel-nodejs-arm64-ver-1-18-1:1',
+      'eu-central-1': 'arn:aws:lambda:eu-central-1:901920570463:layer:aws-otel-nodejs-arm64-ver-1-18-1:1',
+      'eu-north-1': 'arn:aws:lambda:eu-north-1:901920570463:layer:aws-otel-nodejs-arm64-ver-1-18-1:1',
+      'eu-west-1': 'arn:aws:lambda:eu-west-1:901920570463:layer:aws-otel-nodejs-arm64-ver-1-18-1:1',
+      'eu-west-2': 'arn:aws:lambda:eu-west-2:901920570463:layer:aws-otel-nodejs-arm64-ver-1-18-1:1',
+      'eu-west-3': 'arn:aws:lambda:eu-west-3:901920570463:layer:aws-otel-nodejs-arm64-ver-1-18-1:1',
+      'sa-east-1': 'arn:aws:lambda:sa-east-1:901920570463:layer:aws-otel-nodejs-arm64-ver-1-18-1:1',
+      'us-east-1': 'arn:aws:lambda:us-east-1:901920570463:layer:aws-otel-nodejs-arm64-ver-1-18-1:1',
+      'us-east-2': 'arn:aws:lambda:us-east-2:901920570463:layer:aws-otel-nodejs-arm64-ver-1-18-1:1',
+      'us-west-1': 'arn:aws:lambda:us-west-1:901920570463:layer:aws-otel-nodejs-arm64-ver-1-18-1:1',
+      'us-west-2': 'arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-nodejs-arm64-ver-1-18-1:1',
+    },
+  },
   '1.17.1': {
     x86_64: {
       // 'af-south-1': 'arn:aws:lambda:af-south-1:901920570463:layer:aws-otel-nodejs-amd64-ver-1-17-1:1',
@@ -2237,6 +2351,44 @@ const ADOT_LAMBDA_LAYER_JAVASCRIPT_SDK_ARNS: { [version: string]: { [arch: strin
 };
 
 const ADOT_LAMBDA_LAYER_PYTHON_SDK_ARNS: { [version: string]: { [arch: string]: { [region: string]: string } } } = {
+  '1.21.0': {
+    x86_64: {
+      'ap-northeast-1': 'arn:aws:lambda:ap-northeast-1:901920570463:layer:aws-otel-python-amd64-ver-1-21-0:1',
+      'ap-northeast-2': 'arn:aws:lambda:ap-northeast-2:901920570463:layer:aws-otel-python-amd64-ver-1-21-0:1',
+      'ap-south-1': 'arn:aws:lambda:ap-south-1:901920570463:layer:aws-otel-python-amd64-ver-1-21-0:1',
+      'ap-southeast-1': 'arn:aws:lambda:ap-southeast-1:901920570463:layer:aws-otel-python-amd64-ver-1-21-0:1',
+      'ap-southeast-2': 'arn:aws:lambda:ap-southeast-2:901920570463:layer:aws-otel-python-amd64-ver-1-21-0:1',
+      'ca-central-1': 'arn:aws:lambda:ca-central-1:901920570463:layer:aws-otel-python-amd64-ver-1-21-0:1',
+      'eu-central-1': 'arn:aws:lambda:eu-central-1:901920570463:layer:aws-otel-python-amd64-ver-1-21-0:1',
+      'eu-north-1': 'arn:aws:lambda:eu-north-1:901920570463:layer:aws-otel-python-amd64-ver-1-21-0:1',
+      'eu-west-1': 'arn:aws:lambda:eu-west-1:901920570463:layer:aws-otel-python-amd64-ver-1-21-0:1',
+      'eu-west-2': 'arn:aws:lambda:eu-west-2:901920570463:layer:aws-otel-python-amd64-ver-1-21-0:1',
+      'eu-west-3': 'arn:aws:lambda:eu-west-3:901920570463:layer:aws-otel-python-amd64-ver-1-21-0:1',
+      'sa-east-1': 'arn:aws:lambda:sa-east-1:901920570463:layer:aws-otel-python-amd64-ver-1-21-0:1',
+      'us-east-1': 'arn:aws:lambda:us-east-1:901920570463:layer:aws-otel-python-amd64-ver-1-21-0:1',
+      'us-east-2': 'arn:aws:lambda:us-east-2:901920570463:layer:aws-otel-python-amd64-ver-1-21-0:1',
+      'us-west-1': 'arn:aws:lambda:us-west-1:901920570463:layer:aws-otel-python-amd64-ver-1-21-0:1',
+      'us-west-2': 'arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-python-amd64-ver-1-21-0:1',
+    },
+    arm64: {
+      'ap-northeast-1': 'arn:aws:lambda:ap-northeast-1:901920570463:layer:aws-otel-python-arm64-ver-1-21-0:1',
+      'ap-northeast-2': 'arn:aws:lambda:ap-northeast-2:901920570463:layer:aws-otel-python-arm64-ver-1-21-0:1',
+      'ap-south-1': 'arn:aws:lambda:ap-south-1:901920570463:layer:aws-otel-python-arm64-ver-1-21-0:1',
+      'ap-southeast-1': 'arn:aws:lambda:ap-southeast-1:901920570463:layer:aws-otel-python-arm64-ver-1-21-0:1',
+      'ap-southeast-2': 'arn:aws:lambda:ap-southeast-2:901920570463:layer:aws-otel-python-arm64-ver-1-21-0:1',
+      'ca-central-1': 'arn:aws:lambda:ca-central-1:901920570463:layer:aws-otel-python-arm64-ver-1-21-0:1',
+      'eu-central-1': 'arn:aws:lambda:eu-central-1:901920570463:layer:aws-otel-python-arm64-ver-1-21-0:1',
+      'eu-north-1': 'arn:aws:lambda:eu-north-1:901920570463:layer:aws-otel-python-arm64-ver-1-21-0:1',
+      'eu-west-1': 'arn:aws:lambda:eu-west-1:901920570463:layer:aws-otel-python-arm64-ver-1-21-0:1',
+      'eu-west-2': 'arn:aws:lambda:eu-west-2:901920570463:layer:aws-otel-python-arm64-ver-1-21-0:1',
+      'eu-west-3': 'arn:aws:lambda:eu-west-3:901920570463:layer:aws-otel-python-arm64-ver-1-21-0:1',
+      'sa-east-1': 'arn:aws:lambda:sa-east-1:901920570463:layer:aws-otel-python-arm64-ver-1-21-0:1',
+      'us-east-1': 'arn:aws:lambda:us-east-1:901920570463:layer:aws-otel-python-arm64-ver-1-21-0:1',
+      'us-east-2': 'arn:aws:lambda:us-east-2:901920570463:layer:aws-otel-python-arm64-ver-1-21-0:1',
+      'us-west-1': 'arn:aws:lambda:us-west-1:901920570463:layer:aws-otel-python-arm64-ver-1-21-0:1',
+      'us-west-2': 'arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-python-arm64-ver-1-21-0:1',
+    },
+  },
   '1.20.0-1': {
     x86_64: {
       // 'af-south-1': 'arn:aws:lambda:af-south-1:901920570463:layer:aws-otel-python-amd64-ver-1-20-0:3',
@@ -2682,6 +2834,44 @@ const ADOT_LAMBDA_LAYER_PYTHON_SDK_ARNS: { [version: string]: { [arch: string]:
 };
 
 const ADOT_LAMBDA_LAYER_GENERIC_ARNS: { [version: string]: { [arch: string]: { [region: string]: string } } } = {
+  '0.90.1': {
+    x86_64: {
+      'ap-northeast-1': 'arn:aws:lambda:ap-northeast-1:901920570463:layer:aws-otel-collector-amd64-ver-0-90-1:1',
+      'ap-northeast-2': 'arn:aws:lambda:ap-northeast-2:901920570463:layer:aws-otel-collector-amd64-ver-0-90-1:1',
+      'ap-south-1': 'arn:aws:lambda:ap-south-1:901920570463:layer:aws-otel-collector-amd64-ver-0-90-1:1',
+      'ap-southeast-1': 'arn:aws:lambda:ap-southeast-1:901920570463:layer:aws-otel-collector-amd64-ver-0-90-1:1',
+      'ap-southeast-2': 'arn:aws:lambda:ap-southeast-2:901920570463:layer:aws-otel-collector-amd64-ver-0-90-1:1',
+      'ca-central-1': 'arn:aws:lambda:ca-central-1:901920570463:layer:aws-otel-collector-amd64-ver-0-90-1:1',
+      'eu-central-1': 'arn:aws:lambda:eu-central-1:901920570463:layer:aws-otel-collector-amd64-ver-0-90-1:1',
+      'eu-north-1': 'arn:aws:lambda:eu-north-1:901920570463:layer:aws-otel-collector-amd64-ver-0-90-1:1',
+      'eu-west-1': 'arn:aws:lambda:eu-west-1:901920570463:layer:aws-otel-collector-amd64-ver-0-90-1:1',
+      'eu-west-2': 'arn:aws:lambda:eu-west-2:901920570463:layer:aws-otel-collector-amd64-ver-0-90-1:1',
+      'eu-west-3': 'arn:aws:lambda:eu-west-3:901920570463:layer:aws-otel-collector-amd64-ver-0-90-1:1',
+      'sa-east-1': 'arn:aws:lambda:sa-east-1:901920570463:layer:aws-otel-collector-amd64-ver-0-90-1:1',
+      'us-east-1': 'arn:aws:lambda:us-east-1:901920570463:layer:aws-otel-collector-amd64-ver-0-90-1:1',
+      'us-east-2': 'arn:aws:lambda:us-east-2:901920570463:layer:aws-otel-collector-amd64-ver-0-90-1:1',
+      'us-west-1': 'arn:aws:lambda:us-west-1:901920570463:layer:aws-otel-collector-amd64-ver-0-90-1:1',
+      'us-west-2': 'arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-collector-amd64-ver-0-90-1:1',
+    },
+    arm64: {
+      'ap-northeast-1': 'arn:aws:lambda:ap-northeast-1:901920570463:layer:aws-otel-collector-arm64-ver-0-90-1:1',
+      'ap-northeast-2': 'arn:aws:lambda:ap-northeast-2:901920570463:layer:aws-otel-collector-arm64-ver-0-90-1:1',
+      'ap-south-1': 'arn:aws:lambda:ap-south-1:901920570463:layer:aws-otel-collector-arm64-ver-0-90-1:1',
+      'ap-southeast-1': 'arn:aws:lambda:ap-southeast-1:901920570463:layer:aws-otel-collector-arm64-ver-0-90-1:1',
+      'ap-southeast-2': 'arn:aws:lambda:ap-southeast-2:901920570463:layer:aws-otel-collector-arm64-ver-0-90-1:1',
+      'ca-central-1': 'arn:aws:lambda:ca-central-1:901920570463:layer:aws-otel-collector-arm64-ver-0-90-1:1',
+      'eu-central-1': 'arn:aws:lambda:eu-central-1:901920570463:layer:aws-otel-collector-arm64-ver-0-90-1:1',
+      'eu-north-1': 'arn:aws:lambda:eu-north-1:901920570463:layer:aws-otel-collector-arm64-ver-0-90-1:1',
+      'eu-west-1': 'arn:aws:lambda:eu-west-1:901920570463:layer:aws-otel-collector-arm64-ver-0-90-1:1',
+      'eu-west-2': 'arn:aws:lambda:eu-west-2:901920570463:layer:aws-otel-collector-arm64-ver-0-90-1:1',
+      'eu-west-3': 'arn:aws:lambda:eu-west-3:901920570463:layer:aws-otel-collector-arm64-ver-0-90-1:1',
+      'sa-east-1': 'arn:aws:lambda:sa-east-1:901920570463:layer:aws-otel-collector-arm64-ver-0-90-1:1',
+      'us-east-1': 'arn:aws:lambda:us-east-1:901920570463:layer:aws-otel-collector-arm64-ver-0-90-1:1',
+      'us-east-2': 'arn:aws:lambda:us-east-2:901920570463:layer:aws-otel-collector-arm64-ver-0-90-1:1',
+      'us-west-1': 'arn:aws:lambda:us-west-1:901920570463:layer:aws-otel-collector-arm64-ver-0-90-1:1',
+      'us-west-2': 'arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-collector-arm64-ver-0-90-1:1',
+    },
+  },
   '0.88.0': {
     x86_64: {
       //'af-south-1': 'arn:aws:lambda:af-south-1:901920570463:layer:aws-otel-collector-amd64-ver-0-88-0:1',