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    +

    Figure2

    Figure 2: Shunting inhibition promotes local network desynchronization and response flexibility. (A) High-density Neuropixels (NPXs) recordings were used to compare spiking activity in the same cortical neurons under awake and anesthetized conditions. (B) Example raster plots show the spiking activity of a population of neurons in somatosensory cortex (SS). Spiking activity is shown across three stimulation trials for each condition. diff --git a/blog/archive/index.html b/blog/archive/index.html index dbd5ca7..15c311b 100644 --- a/blog/archive/index.html +++ b/blog/archive/index.html @@ -5,7 +5,7 @@ Archive | DANDI - + diff --git a/blog/atom.xml b/blog/atom.xml index 4335bfb..0bdcae3 100644 --- a/blog/atom.xml +++ b/blog/atom.xml @@ -48,7 +48,7 @@ They confirmed this hypothesis by locally injecting an AMPA receptor antagonist By reanalyzing this data and manipulating a computational network model, they found that setting the EGABAAR parameter to the shunting regime (-60mV) reproduced spiking responses similar to those seen in awake mice (Figure 2 B&F left). Conversely, setting it to the hyperpolarizing regime (-80mV) mimicked the responses seen in anesthetized mice (Figure 2 B&F right). This data provides evidence for the hypothesized bidirectional relationship.

    -

    Figure2

    +

    Figure2

    Figure 2: Shunting inhibition promotes local network desynchronization and response flexibility. (A) High-density Neuropixels (NPXs) recordings were used to compare spiking activity in the same cortical neurons under awake and anesthetized conditions. (B) Example raster plots show the spiking activity of a population of neurons in somatosensory cortex (SS). Spiking activity is shown across three stimulation trials for each condition. diff --git a/blog/authors/index.html b/blog/authors/index.html index 814d047..3eb67ff 100644 --- a/blog/authors/index.html +++ b/blog/authors/index.html @@ -5,7 +5,7 @@ Authors | DANDI - + diff --git a/blog/first-blog-post/index.html b/blog/first-blog-post/index.html index feaad84..f824f38 100644 --- a/blog/first-blog-post/index.html +++ b/blog/first-blog-post/index.html @@ -5,7 +5,7 @@ First Blog Post | DANDI - + diff --git a/blog/index.html b/blog/index.html index ba00191..b02845f 100644 --- a/blog/index.html +++ b/blog/index.html @@ -5,7 +5,7 @@ Blog | DANDI - + @@ -44,7 +44,7 @@

    +

    Figure2

    Figure 2: Shunting inhibition promotes local network desynchronization and response flexibility. (A) High-density Neuropixels (NPXs) recordings were used to compare spiking activity in the same cortical neurons under awake and anesthetized conditions. (B) Example raster plots show the spiking activity of a population of neurons in somatosensory cortex (SS). Spiking activity is shown across three stimulation trials for each condition. diff --git a/blog/rss.xml b/blog/rss.xml index 124a023..097c6ed 100644 --- a/blog/rss.xml +++ b/blog/rss.xml @@ -49,7 +49,7 @@ They confirmed this hypothesis by locally injecting an AMPA receptor antagonist By reanalyzing this data and manipulating a computational network model, they found that setting the EGABAAR parameter to the shunting regime (-60mV) reproduced spiking responses similar to those seen in awake mice (Figure 2 B&F left). Conversely, setting it to the hyperpolarizing regime (-80mV) mimicked the responses seen in anesthetized mice (Figure 2 B&F right). This data provides evidence for the hypothesized bidirectional relationship.

    -

    Figure2

    +

    Figure2

    Figure 2: Shunting inhibition promotes local network desynchronization and response flexibility. (A) High-density Neuropixels (NPXs) recordings were used to compare spiking activity in the same cortical neurons under awake and anesthetized conditions. (B) Example raster plots show the spiking activity of a population of neurons in somatosensory cortex (SS). Spiking activity is shown across three stimulation trials for each condition. diff --git a/blog/tags/docusaurus/index.html b/blog/tags/docusaurus/index.html index d0bd3ea..fa33c29 100644 --- a/blog/tags/docusaurus/index.html +++ b/blog/tags/docusaurus/index.html @@ -5,7 +5,7 @@ One post tagged with "Docusaurus" | DANDI - + diff --git a/blog/tags/hola/index.html b/blog/tags/hola/index.html index 1d1f36c..152c70c 100644 --- a/blog/tags/hola/index.html +++ b/blog/tags/hola/index.html @@ -5,7 +5,7 @@ One post tagged with "Hola" | DANDI - + diff --git a/blog/tags/index.html b/blog/tags/index.html index b40e2df..74dc0f3 100644 --- a/blog/tags/index.html +++ b/blog/tags/index.html @@ -5,7 +5,7 @@ Tags | DANDI - + diff --git a/blog/tags/reuse-showcase/index.html b/blog/tags/reuse-showcase/index.html index 1a2a3b9..f060f7e 100644 --- a/blog/tags/reuse-showcase/index.html +++ b/blog/tags/reuse-showcase/index.html @@ -5,7 +5,7 @@ One post tagged with "reuse showcase" | DANDI - + @@ -44,7 +44,7 @@

    +

    Figure2

    Figure 2: Shunting inhibition promotes local network desynchronization and response flexibility. (A) High-density Neuropixels (NPXs) recordings were used to compare spiking activity in the same cortical neurons under awake and anesthetized conditions. (B) Example raster plots show the spiking activity of a population of neurons in somatosensory cortex (SS). Spiking activity is shown across three stimulation trials for each condition. diff --git a/docs/welcome/index.html b/docs/welcome/index.html index 31f18ee..e091746 100644 --- a/docs/welcome/index.html +++ b/docs/welcome/index.html @@ -5,7 +5,7 @@ Welcome | DANDI - + diff --git a/index.html b/index.html index 2179c4a..13f45ee 100644 --- a/index.html +++ b/index.html @@ -5,7 +5,7 @@ Hello from DANDI | DANDI - + diff --git a/news/index.html b/news/index.html index e7bea37..84b8271 100644 --- a/news/index.html +++ b/news/index.html @@ -5,7 +5,7 @@ News | DANDI - + diff --git a/team/index.html b/team/index.html index 82fd1bd..3cbd655 100644 --- a/team/index.html +++ b/team/index.html @@ -5,7 +5,7 @@ Team Members | DANDI - +

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0]},h=[{value:"The Original Study: Peering into the Anesthetized Brain",id:"the-original-study-peering-into-the-anesthetized-brain",level:2},{value:"A New Perspective: Zooming in on Synaptic Potentials",id:"a-new-perspective-zooming-in-on-synaptic-potentials",level:2},{value:"Bridging Scales: From Synapses to Networks",id:"bridging-scales-from-synapses-to-networks",level:2},{value:"Engaging with the Open Science Community",id:"engaging-with-the-open-science-community",level:2}];function d(e){const t={a:"a",blockquote:"blockquote",em:"em",h2:"h2",img:"img",p:"p",strong:"strong",...(0,s.R)(),...e.components};return(0,a.jsxs)(a.Fragment,{children:[(0,a.jsx)(t.p,{children:(0,a.jsx)(t.em,{children:"Data Reuse Highlight: This blog entry is part of an ongoing series that showcases examples of data reuse and reanalysis using publicly available data on DANDI."})}),"\n",(0,a.jsxs)(t.p,{children:["Have you ever wondered what happens to your brain when you're under anesthesia? What neural changes coincide with that dramatic loss of consciousness?\nIn this post, we'll explore how two different research teams investigated this fascinating question using the same data from DANDI Archive's ",(0,a.jsx)(t.a,{href:"https://dandiarchive.org/dandiset/000458",children:"Dandiset #000458"}),"."]}),"\n",(0,a.jsx)(t.h2,{id:"the-original-study-peering-into-the-anesthetized-brain",children:"The Original Study: Peering into the Anesthetized Brain"}),"\n",(0,a.jsxs)(t.p,{children:["In June 2023, Dr. Leslie Claar and colleagues from the Allen Institute published their groundbreaking findings in the journal ",(0,a.jsx)(t.em,{children:"eLife"})," in the article, ",(0,a.jsx)(t.a,{href:"https://doi.org/10.7554/eLife.84630.3",children:"\u201cCortico-thalamo-cortical interactions modulate electrically evoked EEG responses in mice\u201d"}),".\nTheir approach was comprehensive: they stimulated mouse cortex while simultaneously recording with ",(0,a.jsx)(t.a,{href:"https://doi.org/10.1016/B978-141603618-0.10024-4",children:"electroencephelography"})," (EEG) and ",(0,a.jsx)(t.a,{href:"https://www.neuropixels.org/",children:"neuropixels probes"}),", comparing brain activity during wakefulness and under isoflurane anesthesia."]}),"\n",(0,a.jsxs)(t.p,{children:["In awake mice, Claar's team observed a robust ",(0,a.jsx)(t.a,{href:"https://doi.org/10.4103/0972-6748.57865",children:"event-related potential"})," (ERP) when they delivered pulsatile electrical stimulation to deep layers of the ",(0,a.jsx)(t.a,{href:"https://doi.org/10.1016/B0-08-043076-7/03460-4",children:"motor cortex"})," (Figure 1A left).\nThis ERP coincided with a distinct pattern of neural spiking, both locally in the motor cortex and in distant sensorimotor-related ",(0,a.jsx)(t.a,{href:"https://www.ncbi.nlm.nih.gov/books/NBK549908",children:"thalamic nuclei"})," (Figure 1B left). The response followed a three-act play: initial excitation within 25 milliseconds, a period of quiescence for about 125 milliseconds, and finally, a strong rebound excitation before returning to baseline.\nWhen the mice were under anesthesia, the story changed dramatically.\nThe ERP's amplitude and complexity plummeted in response to the same stimulation (Figure 1A right).\nWhile the initial excitation persisted in the motor cortex, it vanished in the thalamic nuclei (Figure 1B right).\nThe rebound excitation, along with its corresponding ERP component at around 180 milliseconds, was significantly weakened."]}),"\n",(0,a.jsx)(t.p,{children:"Based on these observations, Claar and colleagues proposed that interactions between the cortex and thalamus are responsible for the rebound excitation phase of the stimulus response.\nThis rebound excitation, in turn, drives the second component of the ERP \u2013 a crucial signal of wakefulness."}),"\n",(0,a.jsx)(t.p,{children:"After their experiments, Claar and her team published their data on DANDI, ensuring that it would remain publicly accessible for any future researchers who wanted to use it. The dataset and manuscript were prepared and published concurrently, which ensured that the dataset included all the paper-relevant data and metadata and curious reviewers could inspect the data directly if they so wished."}),"\n",(0,a.jsx)(t.p,{children:(0,a.jsx)(t.img,{alt:"Figure1",src:n(4059).A+"",width:"1500",height:"582"})}),"\n",(0,a.jsx)(t.p,{children:(0,a.jsxs)(t.em,{children:[(0,a.jsx)(t.strong,{children:"Figure 1: Brain state modulates the ERP via cortico-thalamo-cortical interactions."}),"\n(A) Butterfly plot of ERPs during non-running (quiet wakefulness), running (active wakefulness), and isoflurane-anesthetized states.\n(B) Normalized firing rate, reported as a z-score of the average, pre-stimulus firing rate, of all RS neurons recorded by the Neuropixels probes targeting the stimulated cortex (MO) and SM-TH. Reproduced from \u201cCortico-thalamo-cortical interactions modulate electrically evoked EEG responses in mice\u201d."]})}),"\n",(0,a.jsx)(t.h2,{id:"a-new-perspective-zooming-in-on-synaptic-potentials",children:"A New Perspective: Zooming in on Synaptic Potentials"}),"\n",(0,a.jsxs)(t.p,{children:["Just a few months later, in November 2023, Drs. Richard Burman, Paul Brodersen and colleagues at the University of Oxford wrote a paper that included reanalysis of this data.\nTheir findings were published in the journal ",(0,a.jsx)(t.em,{children:"Neuron"})," with the article, ",(0,a.jsxs)(t.a,{href:"https://doi.org/10.1016/j.neuron.2023.08.005",children:["\u201cActive cortical networks promote shunting fast synaptic inhibition ",(0,a.jsx)(t.em,{children:"in vivo"}),"\u201d"]}),".\nThey also investigated the effects of anesthesia on brain activity, but narrowed their focus to the differential function of a specific receptor subtype: ",(0,a.jsxs)(t.a,{href:"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2648504/",children:["GABA",(0,a.jsx)("sub",{children:"A"}),"R"]}),"."]}),"\n",(0,a.jsxs)(t.p,{children:["Burman and Brodersen's team used a technique called ",(0,a.jsxs)(t.a,{href:"https://doi.org/10.1254/fpj.113.339",children:[(0,a.jsx)(t.em,{children:"in vivo"})," gramicidin perforated patch-clamp recording"]}),", combined with ",(0,a.jsx)(t.a,{href:"https://doi.org/10.1097/ICU.0000000000000140",children:"optogenetic"})," activation of GABAergic synaptic inputs.\nThey compared the GABA",(0,a.jsx)("sub",{children:"A"})," receptor equilibrium potential (E",(0,a.jsxs)("sub",{children:["GABA",(0,a.jsx)("sub",{children:"A"}),"R"]}),") in mice during wakefulness and under urethane anesthesia."]}),"\n",(0,a.jsxs)(t.p,{children:["Their findings were striking. In anesthetized mice, GABA",(0,a.jsx)("sub",{children:"A"}),' receptor responses were significantly hyperpolarizing, with an average equilibrium potential of -80.8mV.\nIn awake mice, however, these responses could be either hyperpolarizing or depolarizing, averaging at -63.9mV \u2013 a state known as the "',(0,a.jsx)(t.a,{href:"https://en.wikipedia.org/wiki/Shunting_inhibition",children:"shunting"}),'" regime.']}),"\n",(0,a.jsxs)(t.p,{children:["Burman et al. hypothesized that the higher network activity in the awake state leads to a higher concentration of intracellular chloride, which in turn depolarizes the GABA",(0,a.jsx)("sub",{children:"A"})," receptor equilibrium potential.\nAnesthesia, by broadly suppressing neural activity, indirectly hyperpolarizes E",(0,a.jsxs)("sub",{children:["GABA",(0,a.jsx)("sub",{children:"A"}),"R"]}),".\nThey confirmed this hypothesis by locally injecting an AMPA receptor antagonist (",(0,a.jsx)(t.a,{href:"https://www.tocris.com/products/nbqx-disodium-salt_1044",children:"NBQX"}),") which suppressed network activity, and hyperpolarized E",(0,a.jsxs)("sub",{children:["GABA",(0,a.jsx)("sub",{children:"A"}),"R"]})," to -78.0mV."]}),"\n",(0,a.jsxs)(t.p,{children:["The Oxford researchers also hypothesized that changes in E",(0,a.jsxs)("sub",{children:["GABA",(0,a.jsx)("sub",{children:"A"}),"R"]})," would lead to changes in network activity, resulting in a bi-directional relationship between the two quantities."]}),"\n",(0,a.jsxs)(t.p,{children:["To test this hypothesis, Burman and Brodersen's team turned to the data from ",(0,a.jsx)(t.a,{href:"https://dandiarchive.org/dandiset/000458",children:"Dandiset #000458"})," \u2013 the very same dataset used by Claar et al.\nBy reanalyzing this data and manipulating a computational network model, they found that setting the E",(0,a.jsxs)("sub",{children:["GABA",(0,a.jsx)("sub",{children:"A"}),"R"]})," parameter to the shunting regime (-60mV) reproduced spiking responses similar to those seen in awake mice (Figure 2 B&F left).\nConversely, setting it to the hyperpolarizing regime (-80mV) mimicked the responses seen in anesthetized mice (Figure 2 B&F right).\nThis data provides evidence for the hypothesized bidirectional relationship."]}),"\n",(0,a.jsx)(t.p,{children:(0,a.jsx)(t.img,{alt:"Figure2",src:n(4059).A+"",width:"1500",height:"582"})}),"\n",(0,a.jsx)(t.p,{children:(0,a.jsxs)(t.em,{children:[(0,a.jsx)(t.strong,{children:"Figure 2: Shunting inhibition promotes local network desynchronization and response flexibility."}),"\n(A) High-density Neuropixels (NPXs) recordings were used to compare spiking activity in the same cortical neurons under awake and anesthetized conditions.\n(B) Example raster plots show the spiking activity of a population of neurons in somatosensory cortex (SS). Spiking activity is shown across three stimulation trials for each condition.\n(C) Neuronal synchrony on a trial-to-trial basis (top) and the entropy of the peri-stimulus histogram (bottom) were calculated for each of the 662 recorded neurons under awake and anesthetized conditions (17 probe recordings in 16 mice). Each dot corresponds to a single neuron and the dashed line indicates the line of equality.\n(D) Neurons in the awake condition exhibited lower synchrony (top; Aw.: 0.325 \xb1 0.005 vs. An.: 0.393 \xb1 0.007, p < 0.001, paired t test) and higher entropy (bottom; Aw.: 1.347 \xb1 0.018 nats vs. An.: 1.111 \xb1 0.020 nats, p < 0.001, paired t test).\n(E) Schematic of network model consisting of interconnected excitatory pyramidal neurons (Pyr.) and inhibitory interneurons (IN). EGABAAR in the pyramidal neurons was adjusted relative to the RMP to create two different conditions: a shunting (Shunt.) and a hyperpolarizing (Hyperpol.) EGABAAR condition. Spiking activity was evoked by delivering brief depolarizing currents (input) of varying amplitudes to each neuron in the network.\n(F) Raster plots for the same population of pyramidal neurons (n = 50) in the shunting (left) and hyperpolarizing (right) EGABAAR conditions.\n(G) Synchrony (top) and entropy (bottom) for pyramidal neurons in the shunting and hyperpolarizing EGABAAR conditions (n = 1,000 randomly selected). Dashed line indicates the line of equality. (H) Neurons in the shunting EGABAAR condition exhibited lower synchrony (Shunt: 0.784 \xb1 0.001 vs. Hyperpol: 0.881 \xb1 0.001, p < 0.001, paired t test) and higher entropy (Shunt: 0.451 \xb1 0.002 nats vs. Hyperpol: 0.197 \xb1 0.002 nats, p < 0.001, paired t test) (n = 16,000 pyramidal neurons from the model). \u2217\u2217\u2217p < 0.001. MO, motor cortex; Stim., electrical stimulation; TH, thalamus. Reproduced from \u201cActive cortical networks promote shunting fast synaptic inhibition in vivo\u201d."]})}),"\n",(0,a.jsx)(t.h2,{id:"bridging-scales-from-synapses-to-networks",children:"Bridging Scales: From Synapses to Networks"}),"\n",(0,a.jsxs)(t.p,{children:["Together, these two studies provide a rich, multi-level account of what happens to brain activity under anesthesia. They span from macroscale brain signals recorded by EEG all the way down to microscale synaptic responses from a single receptor type.\nThe population spiking data from ",(0,a.jsx)(t.a,{href:"https://dandiarchive.org/dandiset/000458",children:"Dandiset #000458"})," serves as a crucial bridge between these levels, allowing Burman et al. to explicitly connect their work to the broader field of anesthesia research."]}),"\n",(0,a.jsx)(t.p,{children:"However, it's important to note some key differences between the two studies. Claar et al. used isoflurane anesthesia and stimulated layers 5/6 of the cortex, while Burman et al. used urethane anesthesia and recorded from layer 2/3.\nAdditionally, while Claar et al. found the most dramatic differences between waking and anesthetized states around 180 ms after stimulation, Burman et al. focused their reanalysis on the initial spiking response within 15 ms of stimulation."}),"\n",(0,a.jsxs)(t.p,{children:["These differences highlight the potential for further reanalysis of ",(0,a.jsx)(t.a,{href:"https://dandiarchive.org/dandiset/000458",children:"Dandiset #000458"}),".\nFuture studies could explore more similar experimental conditions, conduct a more thorough treatment of relevant response time periods, or investigate different aspects of anesthesia's effects altogether."]}),"\n",(0,a.jsx)(t.h2,{id:"engaging-with-the-open-science-community",children:"Engaging with the Open Science Community"}),"\n",(0,a.jsx)(t.p,{children:"For those inspired by the work of Claar, Burman and Brodersen, and their teams, the journey doesn\u2019t end here.\nWe encourage readers from all backgrounds to take advantage of this open science platform by publishing and reanalyzing data on DANDI.\nWe are confident that it will be a smooth and rewarding experience, but don't just take our word for it -- listen to what Dr. Leslie Claar and Dr. Paul Brodersen have to say,"}),"\n",(0,a.jsxs)(t.blockquote,{children:["\n",(0,a.jsx)(t.p,{children:'"After organizing our dataset into NWB format, validating and uploading our files was straightforward using a few simple commands from the DANDI Python Client.\nThe tools are well-documented, which made the process of uploading and publishing quite smooth.\nWithin a few months of publishing on DANDI, others were already interested in using our dataset."\n-- Dr. Leslie Claar'}),"\n"]}),"\n",(0,a.jsxs)(t.blockquote,{children:["\n",(0,a.jsx)(t.p,{children:"\u201cThe DANDI archive is a fantastic resource with great tooling and exhaustive documentation. Its command line interface makes data retrieval and synchronization as simple as possible (but not simpler!).\nThe standardized and well-documented formats for electrophysiological and neuroimaging data ensure that anyone can quickly find their way around a new archive without requiring extensive feedback from the authors that originally collected the data.\nThese features make the DANDI archive a treasure trove of useful and reusable data, unlike the unfortunately still commonplace unstructured data dumps on many other platforms or -- worst of all -- university servers.\u201d\n-- Dr. Paul Brodersen and Professor Colin Akerman"}),"\n"]}),"\n",(0,a.jsxs)(t.p,{children:["To get you started on your own reanalysis project, we've prepared a Jupyter ",(0,a.jsx)(t.a,{href:"https://github.com/dandi/example-notebooks/blob/master/000458/AllenInstitute/reanalysis.ipynb",children:"notebook"})," demonstrating some basic reanalysis of ",(0,a.jsx)(t.a,{href:"https://dandiarchive.org/dandiset/000458",children:"Dandiset #000458"}),".\nThis tool will help you dive right into the data, guiding you through the initial steps of exploration and analysis.\nWe have also prepared a Neurosift ",(0,a.jsx)(t.a,{href:"https://github.com/dandi/example-notebooks/blob/master/000458/FlatironInstitute/PSTH_neurosift_exploration.md",children:"walkthrough"})," which demonstrates how to use Neurosift to quickly and easily explore the NWB files in this dataset without even leaving the browser."]}),"\n",(0,a.jsx)(t.p,{children:"By engaging with this open science platform, you become part of a larger community of researchers who are building on each other's work and driving the field forward. In the world of open science, collaboration and curiosity drive discovery\u2014so why not see where the data takes you?"})]})}function c(e={}){const{wrapper:t}={...(0,s.R)(),...e.components};return t?(0,a.jsx)(t,{...e,children:(0,a.jsx)(d,{...e})}):d(e)}},4059:(e,t,n)=>{n.d(t,{A:()=>i});const i=n.p+"assets/images/data_reuse_000458_elife-fig7-14d309a15c91768081366dc94ab7d5d7.jpg"},8453:(e,t,n)=>{n.d(t,{R:()=>r,x:()=>o});var i=n(6540);const a={},s=i.createContext(a);function r(e){const t=i.useContext(s);return i.useMemo((function(){return"function"==typeof e?e(t):{...t,...e}}),[t,e])}function o(e){let t;return t=e.disableParentContext?"function"==typeof e.components?e.components(a):e.components||a:r(e.components),i.createElement(s.Provider,{value:t},e.children)}},5848:e=>{e.exports=JSON.parse('{"permalink":"/dandi-docusaurus-example/blog/2024/09/09/data_reuse_000458","editUrl":"https://github.com/facebook/docusaurus/tree/main/packages/create-docusaurus/templates/shared/blog/2024-09-09-data_reuse_000458.md","source":"@site/blog/2024-09-09-data_reuse_000458.md","title":"Unraveling Anesthesia\'s Effect on Brain Activity","description":"Data Reuse Highlight: This blog entry is part of an ongoing series that showcases examples of data reuse and reanalysis using publicly available data on DANDI.","date":"2024-09-09T00:00:00.000Z","tags":[{"inline":true,"label":"reuse showcase","permalink":"/dandi-docusaurus-example/blog/tags/reuse-showcase"}],"readingTime":8.45,"hasTruncateMarker":false,"authors":[{"name":"Paul Adkisson","key":null,"page":null}],"frontMatter":{"title":"Unraveling Anesthesia\'s Effect on Brain Activity","author":"Paul Adkisson","tags":["reuse showcase"]},"unlisted":false,"nextItem":{"title":"First Blog Post","permalink":"/dandi-docusaurus-example/blog/first-blog-post"}}')}}]); \ No newline at end of file +"use strict";(self.webpackChunkdandi_website=self.webpackChunkdandi_website||[]).push([[321],{3444:(e,t,n)=>{n.r(t),n.d(t,{assets:()=>l,contentTitle:()=>o,default:()=>c,frontMatter:()=>r,metadata:()=>i,toc:()=>h});var i=n(5848),a=n(4848),s=n(8453);const r={title:"Unraveling Anesthesia's Effect on Brain Activity",author:"Paul Adkisson",tags:["reuse showcase"]},o=void 0,l={authorsImageUrls:[void 0]},h=[{value:"The Original Study: Peering into the Anesthetized Brain",id:"the-original-study-peering-into-the-anesthetized-brain",level:2},{value:"A New Perspective: Zooming in on Synaptic Potentials",id:"a-new-perspective-zooming-in-on-synaptic-potentials",level:2},{value:"Bridging Scales: From Synapses to Networks",id:"bridging-scales-from-synapses-to-networks",level:2},{value:"Engaging with the Open Science Community",id:"engaging-with-the-open-science-community",level:2}];function d(e){const t={a:"a",blockquote:"blockquote",em:"em",h2:"h2",img:"img",p:"p",strong:"strong",...(0,s.R)(),...e.components};return(0,a.jsxs)(a.Fragment,{children:[(0,a.jsx)(t.p,{children:(0,a.jsx)(t.em,{children:"Data Reuse Highlight: This blog entry is part of an ongoing series that showcases examples of data reuse and reanalysis using publicly available data on DANDI."})}),"\n",(0,a.jsxs)(t.p,{children:["Have you ever wondered what happens to your brain when you're under anesthesia? What neural changes coincide with that dramatic loss of consciousness?\nIn this post, we'll explore how two different research teams investigated this fascinating question using the same data from DANDI Archive's ",(0,a.jsx)(t.a,{href:"https://dandiarchive.org/dandiset/000458",children:"Dandiset #000458"}),"."]}),"\n",(0,a.jsx)(t.h2,{id:"the-original-study-peering-into-the-anesthetized-brain",children:"The Original Study: Peering into the Anesthetized Brain"}),"\n",(0,a.jsxs)(t.p,{children:["In June 2023, Dr. Leslie Claar and colleagues from the Allen Institute published their groundbreaking findings in the journal ",(0,a.jsx)(t.em,{children:"eLife"})," in the article, ",(0,a.jsx)(t.a,{href:"https://doi.org/10.7554/eLife.84630.3",children:"\u201cCortico-thalamo-cortical interactions modulate electrically evoked EEG responses in mice\u201d"}),".\nTheir approach was comprehensive: they stimulated mouse cortex while simultaneously recording with ",(0,a.jsx)(t.a,{href:"https://doi.org/10.1016/B978-141603618-0.10024-4",children:"electroencephelography"})," (EEG) and ",(0,a.jsx)(t.a,{href:"https://www.neuropixels.org/",children:"neuropixels probes"}),", comparing brain activity during wakefulness and under isoflurane anesthesia."]}),"\n",(0,a.jsxs)(t.p,{children:["In awake mice, Claar's team observed a robust ",(0,a.jsx)(t.a,{href:"https://doi.org/10.4103/0972-6748.57865",children:"event-related potential"})," (ERP) when they delivered pulsatile electrical stimulation to deep layers of the ",(0,a.jsx)(t.a,{href:"https://doi.org/10.1016/B0-08-043076-7/03460-4",children:"motor cortex"})," (Figure 1A left).\nThis ERP coincided with a distinct pattern of neural spiking, both locally in the motor cortex and in distant sensorimotor-related ",(0,a.jsx)(t.a,{href:"https://www.ncbi.nlm.nih.gov/books/NBK549908",children:"thalamic nuclei"})," (Figure 1B left). The response followed a three-act play: initial excitation within 25 milliseconds, a period of quiescence for about 125 milliseconds, and finally, a strong rebound excitation before returning to baseline.\nWhen the mice were under anesthesia, the story changed dramatically.\nThe ERP's amplitude and complexity plummeted in response to the same stimulation (Figure 1A right).\nWhile the initial excitation persisted in the motor cortex, it vanished in the thalamic nuclei (Figure 1B right).\nThe rebound excitation, along with its corresponding ERP component at around 180 milliseconds, was significantly weakened."]}),"\n",(0,a.jsx)(t.p,{children:"Based on these observations, Claar and colleagues proposed that interactions between the cortex and thalamus are responsible for the rebound excitation phase of the stimulus response.\nThis rebound excitation, in turn, drives the second component of the ERP \u2013 a crucial signal of wakefulness."}),"\n",(0,a.jsx)(t.p,{children:"After their experiments, Claar and her team published their data on DANDI, ensuring that it would remain publicly accessible for any future researchers who wanted to use it. The dataset and manuscript were prepared and published concurrently, which ensured that the dataset included all the paper-relevant data and metadata and curious reviewers could inspect the data directly if they so wished."}),"\n",(0,a.jsx)(t.p,{children:(0,a.jsx)(t.img,{alt:"Figure1",src:n(4059).A+"",width:"1500",height:"582"})}),"\n",(0,a.jsx)(t.p,{children:(0,a.jsxs)(t.em,{children:[(0,a.jsx)(t.strong,{children:"Figure 1: Brain state modulates the ERP via cortico-thalamo-cortical interactions."}),"\n(A) Butterfly plot of ERPs during non-running (quiet wakefulness), running (active wakefulness), and isoflurane-anesthetized states.\n(B) Normalized firing rate, reported as a z-score of the average, pre-stimulus firing rate, of all RS neurons recorded by the Neuropixels probes targeting the stimulated cortex (MO) and SM-TH. Reproduced from \u201cCortico-thalamo-cortical interactions modulate electrically evoked EEG responses in mice\u201d."]})}),"\n",(0,a.jsx)(t.h2,{id:"a-new-perspective-zooming-in-on-synaptic-potentials",children:"A New Perspective: Zooming in on Synaptic Potentials"}),"\n",(0,a.jsxs)(t.p,{children:["Just a few months later, in November 2023, Drs. Richard Burman, Paul Brodersen and colleagues at the University of Oxford wrote a paper that included reanalysis of this data.\nTheir findings were published in the journal ",(0,a.jsx)(t.em,{children:"Neuron"})," with the article, ",(0,a.jsxs)(t.a,{href:"https://doi.org/10.1016/j.neuron.2023.08.005",children:["\u201cActive cortical networks promote shunting fast synaptic inhibition ",(0,a.jsx)(t.em,{children:"in vivo"}),"\u201d"]}),".\nThey also investigated the effects of anesthesia on brain activity, but narrowed their focus to the differential function of a specific receptor subtype: ",(0,a.jsxs)(t.a,{href:"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2648504/",children:["GABA",(0,a.jsx)("sub",{children:"A"}),"R"]}),"."]}),"\n",(0,a.jsxs)(t.p,{children:["Burman and Brodersen's team used a technique called ",(0,a.jsxs)(t.a,{href:"https://doi.org/10.1254/fpj.113.339",children:[(0,a.jsx)(t.em,{children:"in vivo"})," gramicidin perforated patch-clamp recording"]}),", combined with ",(0,a.jsx)(t.a,{href:"https://doi.org/10.1097/ICU.0000000000000140",children:"optogenetic"})," activation of GABAergic synaptic inputs.\nThey compared the GABA",(0,a.jsx)("sub",{children:"A"})," receptor equilibrium potential (E",(0,a.jsxs)("sub",{children:["GABA",(0,a.jsx)("sub",{children:"A"}),"R"]}),") in mice during wakefulness and under urethane anesthesia."]}),"\n",(0,a.jsxs)(t.p,{children:["Their findings were striking. In anesthetized mice, GABA",(0,a.jsx)("sub",{children:"A"}),' receptor responses were significantly hyperpolarizing, with an average equilibrium potential of -80.8mV.\nIn awake mice, however, these responses could be either hyperpolarizing or depolarizing, averaging at -63.9mV \u2013 a state known as the "',(0,a.jsx)(t.a,{href:"https://en.wikipedia.org/wiki/Shunting_inhibition",children:"shunting"}),'" regime.']}),"\n",(0,a.jsxs)(t.p,{children:["Burman et al. hypothesized that the higher network activity in the awake state leads to a higher concentration of intracellular chloride, which in turn depolarizes the GABA",(0,a.jsx)("sub",{children:"A"})," receptor equilibrium potential.\nAnesthesia, by broadly suppressing neural activity, indirectly hyperpolarizes E",(0,a.jsxs)("sub",{children:["GABA",(0,a.jsx)("sub",{children:"A"}),"R"]}),".\nThey confirmed this hypothesis by locally injecting an AMPA receptor antagonist (",(0,a.jsx)(t.a,{href:"https://www.tocris.com/products/nbqx-disodium-salt_1044",children:"NBQX"}),") which suppressed network activity, and hyperpolarized E",(0,a.jsxs)("sub",{children:["GABA",(0,a.jsx)("sub",{children:"A"}),"R"]})," to -78.0mV."]}),"\n",(0,a.jsxs)(t.p,{children:["The Oxford researchers also hypothesized that changes in E",(0,a.jsxs)("sub",{children:["GABA",(0,a.jsx)("sub",{children:"A"}),"R"]})," would lead to changes in network activity, resulting in a bi-directional relationship between the two quantities."]}),"\n",(0,a.jsxs)(t.p,{children:["To test this hypothesis, Burman and Brodersen's team turned to the data from ",(0,a.jsx)(t.a,{href:"https://dandiarchive.org/dandiset/000458",children:"Dandiset #000458"})," \u2013 the very same dataset used by Claar et al.\nBy reanalyzing this data and manipulating a computational network model, they found that setting the E",(0,a.jsxs)("sub",{children:["GABA",(0,a.jsx)("sub",{children:"A"}),"R"]})," parameter to the shunting regime (-60mV) reproduced spiking responses similar to those seen in awake mice (Figure 2 B&F left).\nConversely, setting it to the hyperpolarizing regime (-80mV) mimicked the responses seen in anesthetized mice (Figure 2 B&F right).\nThis data provides evidence for the hypothesized bidirectional relationship."]}),"\n",(0,a.jsx)(t.p,{children:(0,a.jsx)(t.img,{alt:"Figure2",src:n(7287).A+"",width:"3000",height:"2091"})}),"\n",(0,a.jsx)(t.p,{children:(0,a.jsxs)(t.em,{children:[(0,a.jsx)(t.strong,{children:"Figure 2: Shunting inhibition promotes local network desynchronization and response flexibility."}),"\n(A) High-density Neuropixels (NPXs) recordings were used to compare spiking activity in the same cortical neurons under awake and anesthetized conditions.\n(B) Example raster plots show the spiking activity of a population of neurons in somatosensory cortex (SS). Spiking activity is shown across three stimulation trials for each condition.\n(C) Neuronal synchrony on a trial-to-trial basis (top) and the entropy of the peri-stimulus histogram (bottom) were calculated for each of the 662 recorded neurons under awake and anesthetized conditions (17 probe recordings in 16 mice). Each dot corresponds to a single neuron and the dashed line indicates the line of equality.\n(D) Neurons in the awake condition exhibited lower synchrony (top; Aw.: 0.325 \xb1 0.005 vs. An.: 0.393 \xb1 0.007, p < 0.001, paired t test) and higher entropy (bottom; Aw.: 1.347 \xb1 0.018 nats vs. An.: 1.111 \xb1 0.020 nats, p < 0.001, paired t test).\n(E) Schematic of network model consisting of interconnected excitatory pyramidal neurons (Pyr.) and inhibitory interneurons (IN). EGABAAR in the pyramidal neurons was adjusted relative to the RMP to create two different conditions: a shunting (Shunt.) and a hyperpolarizing (Hyperpol.) EGABAAR condition. Spiking activity was evoked by delivering brief depolarizing currents (input) of varying amplitudes to each neuron in the network.\n(F) Raster plots for the same population of pyramidal neurons (n = 50) in the shunting (left) and hyperpolarizing (right) EGABAAR conditions.\n(G) Synchrony (top) and entropy (bottom) for pyramidal neurons in the shunting and hyperpolarizing EGABAAR conditions (n = 1,000 randomly selected). Dashed line indicates the line of equality. (H) Neurons in the shunting EGABAAR condition exhibited lower synchrony (Shunt: 0.784 \xb1 0.001 vs. Hyperpol: 0.881 \xb1 0.001, p < 0.001, paired t test) and higher entropy (Shunt: 0.451 \xb1 0.002 nats vs. Hyperpol: 0.197 \xb1 0.002 nats, p < 0.001, paired t test) (n = 16,000 pyramidal neurons from the model). \u2217\u2217\u2217p < 0.001. MO, motor cortex; Stim., electrical stimulation; TH, thalamus. Reproduced from \u201cActive cortical networks promote shunting fast synaptic inhibition in vivo\u201d."]})}),"\n",(0,a.jsx)(t.h2,{id:"bridging-scales-from-synapses-to-networks",children:"Bridging Scales: From Synapses to Networks"}),"\n",(0,a.jsxs)(t.p,{children:["Together, these two studies provide a rich, multi-level account of what happens to brain activity under anesthesia. They span from macroscale brain signals recorded by EEG all the way down to microscale synaptic responses from a single receptor type.\nThe population spiking data from ",(0,a.jsx)(t.a,{href:"https://dandiarchive.org/dandiset/000458",children:"Dandiset #000458"})," serves as a crucial bridge between these levels, allowing Burman et al. to explicitly connect their work to the broader field of anesthesia research."]}),"\n",(0,a.jsx)(t.p,{children:"However, it's important to note some key differences between the two studies. Claar et al. used isoflurane anesthesia and stimulated layers 5/6 of the cortex, while Burman et al. used urethane anesthesia and recorded from layer 2/3.\nAdditionally, while Claar et al. found the most dramatic differences between waking and anesthetized states around 180 ms after stimulation, Burman et al. focused their reanalysis on the initial spiking response within 15 ms of stimulation."}),"\n",(0,a.jsxs)(t.p,{children:["These differences highlight the potential for further reanalysis of ",(0,a.jsx)(t.a,{href:"https://dandiarchive.org/dandiset/000458",children:"Dandiset #000458"}),".\nFuture studies could explore more similar experimental conditions, conduct a more thorough treatment of relevant response time periods, or investigate different aspects of anesthesia's effects altogether."]}),"\n",(0,a.jsx)(t.h2,{id:"engaging-with-the-open-science-community",children:"Engaging with the Open Science Community"}),"\n",(0,a.jsx)(t.p,{children:"For those inspired by the work of Claar, Burman and Brodersen, and their teams, the journey doesn\u2019t end here.\nWe encourage readers from all backgrounds to take advantage of this open science platform by publishing and reanalyzing data on DANDI.\nWe are confident that it will be a smooth and rewarding experience, but don't just take our word for it -- listen to what Dr. Leslie Claar and Dr. Paul Brodersen have to say,"}),"\n",(0,a.jsxs)(t.blockquote,{children:["\n",(0,a.jsx)(t.p,{children:'"After organizing our dataset into NWB format, validating and uploading our files was straightforward using a few simple commands from the DANDI Python Client.\nThe tools are well-documented, which made the process of uploading and publishing quite smooth.\nWithin a few months of publishing on DANDI, others were already interested in using our dataset."\n-- Dr. Leslie Claar'}),"\n"]}),"\n",(0,a.jsxs)(t.blockquote,{children:["\n",(0,a.jsx)(t.p,{children:"\u201cThe DANDI archive is a fantastic resource with great tooling and exhaustive documentation. Its command line interface makes data retrieval and synchronization as simple as possible (but not simpler!).\nThe standardized and well-documented formats for electrophysiological and neuroimaging data ensure that anyone can quickly find their way around a new archive without requiring extensive feedback from the authors that originally collected the data.\nThese features make the DANDI archive a treasure trove of useful and reusable data, unlike the unfortunately still commonplace unstructured data dumps on many other platforms or -- worst of all -- university servers.\u201d\n-- Dr. Paul Brodersen and Professor Colin Akerman"}),"\n"]}),"\n",(0,a.jsxs)(t.p,{children:["To get you started on your own reanalysis project, we've prepared a Jupyter ",(0,a.jsx)(t.a,{href:"https://github.com/dandi/example-notebooks/blob/master/000458/AllenInstitute/reanalysis.ipynb",children:"notebook"})," demonstrating some basic reanalysis of ",(0,a.jsx)(t.a,{href:"https://dandiarchive.org/dandiset/000458",children:"Dandiset #000458"}),".\nThis tool will help you dive right into the data, guiding you through the initial steps of exploration and analysis.\nWe have also prepared a Neurosift ",(0,a.jsx)(t.a,{href:"https://github.com/dandi/example-notebooks/blob/master/000458/FlatironInstitute/PSTH_neurosift_exploration.md",children:"walkthrough"})," which demonstrates how to use Neurosift to quickly and easily explore the NWB files in this dataset without even leaving the browser."]}),"\n",(0,a.jsx)(t.p,{children:"By engaging with this open science platform, you become part of a larger community of researchers who are building on each other's work and driving the field forward. In the world of open science, collaboration and curiosity drive discovery\u2014so why not see where the data takes you?"})]})}function c(e={}){const{wrapper:t}={...(0,s.R)(),...e.components};return t?(0,a.jsx)(t,{...e,children:(0,a.jsx)(d,{...e})}):d(e)}},7287:(e,t,n)=>{n.d(t,{A:()=>i});const i=n.p+"assets/images/data_reuse_000458_cell-fig4-f46a368caff320b8e49aec5e7b1de701.jpg"},4059:(e,t,n)=>{n.d(t,{A:()=>i});const i=n.p+"assets/images/data_reuse_000458_elife-fig7-14d309a15c91768081366dc94ab7d5d7.jpg"},8453:(e,t,n)=>{n.d(t,{R:()=>r,x:()=>o});var i=n(6540);const a={},s=i.createContext(a);function r(e){const t=i.useContext(s);return i.useMemo((function(){return"function"==typeof e?e(t):{...t,...e}}),[t,e])}function o(e){let t;return t=e.disableParentContext?"function"==typeof e.components?e.components(a):e.components||a:r(e.components),i.createElement(s.Provider,{value:t},e.children)}},5848:e=>{e.exports=JSON.parse('{"permalink":"/dandi-docusaurus-example/blog/2024/09/09/data_reuse_000458","editUrl":"https://github.com/facebook/docusaurus/tree/main/packages/create-docusaurus/templates/shared/blog/2024-09-09-data_reuse_000458.md","source":"@site/blog/2024-09-09-data_reuse_000458.md","title":"Unraveling Anesthesia\'s Effect on Brain Activity","description":"Data Reuse Highlight: This blog entry is part of an ongoing series that showcases examples of data reuse and reanalysis using publicly available data on DANDI.","date":"2024-09-09T00:00:00.000Z","tags":[{"inline":true,"label":"reuse showcase","permalink":"/dandi-docusaurus-example/blog/tags/reuse-showcase"}],"readingTime":8.45,"hasTruncateMarker":false,"authors":[{"name":"Paul Adkisson","key":null,"page":null}],"frontMatter":{"title":"Unraveling Anesthesia\'s Effect on Brain Activity","author":"Paul Adkisson","tags":["reuse showcase"]},"unlisted":false,"nextItem":{"title":"First Blog Post","permalink":"/dandi-docusaurus-example/blog/first-blog-post"}}')}}]); \ No newline at end of file diff --git a/assets/js/39e556df.205734fa.js b/assets/js/39e556df.ce77f074.js similarity index 51% rename from assets/js/39e556df.205734fa.js rename to assets/js/39e556df.ce77f074.js index 1a69a86..2b13e6a 100644 --- a/assets/js/39e556df.205734fa.js +++ b/assets/js/39e556df.ce77f074.js @@ -1 +1 @@ -"use strict";(self.webpackChunkdandi_website=self.webpackChunkdandi_website||[]).push([[110],{8544:(e,t,n)=>{n.r(t),n.d(t,{assets:()=>l,contentTitle:()=>o,default:()=>c,frontMatter:()=>r,metadata:()=>i,toc:()=>h});var i=n(5848),a=n(4848),s=n(8453);const r={title:"Unraveling Anesthesia's Effect on Brain Activity",author:"Paul Adkisson",tags:["reuse showcase"]},o=void 0,l={authorsImageUrls:[void 0]},h=[{value:"The Original Study: Peering into the Anesthetized Brain",id:"the-original-study-peering-into-the-anesthetized-brain",level:2},{value:"A New Perspective: Zooming in on Synaptic Potentials",id:"a-new-perspective-zooming-in-on-synaptic-potentials",level:2},{value:"Bridging Scales: From Synapses to Networks",id:"bridging-scales-from-synapses-to-networks",level:2},{value:"Engaging with the Open Science Community",id:"engaging-with-the-open-science-community",level:2}];function d(e){const t={a:"a",blockquote:"blockquote",em:"em",h2:"h2",img:"img",p:"p",strong:"strong",...(0,s.R)(),...e.components};return(0,a.jsxs)(a.Fragment,{children:[(0,a.jsx)(t.p,{children:(0,a.jsx)(t.em,{children:"Data Reuse Highlight: This blog entry is part of an ongoing series that showcases examples of data reuse and reanalysis using publicly available data on DANDI."})}),"\n",(0,a.jsxs)(t.p,{children:["Have you ever wondered what happens to your brain when you're under anesthesia? What neural changes coincide with that dramatic loss of consciousness?\nIn this post, we'll explore how two different research teams investigated this fascinating question using the same data from DANDI Archive's ",(0,a.jsx)(t.a,{href:"https://dandiarchive.org/dandiset/000458",children:"Dandiset #000458"}),"."]}),"\n",(0,a.jsx)(t.h2,{id:"the-original-study-peering-into-the-anesthetized-brain",children:"The Original Study: Peering into the Anesthetized Brain"}),"\n",(0,a.jsxs)(t.p,{children:["In June 2023, Dr. Leslie Claar and colleagues from the Allen Institute published their groundbreaking findings in the journal ",(0,a.jsx)(t.em,{children:"eLife"})," in the article, ",(0,a.jsx)(t.a,{href:"https://doi.org/10.7554/eLife.84630.3",children:"\u201cCortico-thalamo-cortical interactions modulate electrically evoked EEG responses in mice\u201d"}),".\nTheir approach was comprehensive: they stimulated mouse cortex while simultaneously recording with ",(0,a.jsx)(t.a,{href:"https://doi.org/10.1016/B978-141603618-0.10024-4",children:"electroencephelography"})," (EEG) and ",(0,a.jsx)(t.a,{href:"https://www.neuropixels.org/",children:"neuropixels probes"}),", comparing brain activity during wakefulness and under isoflurane anesthesia."]}),"\n",(0,a.jsxs)(t.p,{children:["In awake mice, Claar's team observed a robust ",(0,a.jsx)(t.a,{href:"https://doi.org/10.4103/0972-6748.57865",children:"event-related potential"})," (ERP) when they delivered pulsatile electrical stimulation to deep layers of the ",(0,a.jsx)(t.a,{href:"https://doi.org/10.1016/B0-08-043076-7/03460-4",children:"motor cortex"})," (Figure 1A left).\nThis ERP coincided with a distinct pattern of neural spiking, both locally in the motor cortex and in distant sensorimotor-related ",(0,a.jsx)(t.a,{href:"https://www.ncbi.nlm.nih.gov/books/NBK549908",children:"thalamic nuclei"})," (Figure 1B left). The response followed a three-act play: initial excitation within 25 milliseconds, a period of quiescence for about 125 milliseconds, and finally, a strong rebound excitation before returning to baseline.\nWhen the mice were under anesthesia, the story changed dramatically.\nThe ERP's amplitude and complexity plummeted in response to the same stimulation (Figure 1A right).\nWhile the initial excitation persisted in the motor cortex, it vanished in the thalamic nuclei (Figure 1B right).\nThe rebound excitation, along with its corresponding ERP component at around 180 milliseconds, was significantly weakened."]}),"\n",(0,a.jsx)(t.p,{children:"Based on these observations, Claar and colleagues proposed that interactions between the cortex and thalamus are responsible for the rebound excitation phase of the stimulus response.\nThis rebound excitation, in turn, drives the second component of the ERP \u2013 a crucial signal of wakefulness."}),"\n",(0,a.jsx)(t.p,{children:"After their experiments, Claar and her team published their data on DANDI, ensuring that it would remain publicly accessible for any future researchers who wanted to use it. The dataset and manuscript were prepared and published concurrently, which ensured that the dataset included all the paper-relevant data and metadata and curious reviewers could inspect the data directly if they so wished."}),"\n",(0,a.jsx)(t.p,{children:(0,a.jsx)(t.img,{alt:"Figure1",src:n(4059).A+"",width:"1500",height:"582"})}),"\n",(0,a.jsx)(t.p,{children:(0,a.jsxs)(t.em,{children:[(0,a.jsx)(t.strong,{children:"Figure 1: Brain state modulates the ERP via cortico-thalamo-cortical interactions."}),"\n(A) Butterfly plot of ERPs during non-running (quiet wakefulness), running (active wakefulness), and isoflurane-anesthetized states.\n(B) Normalized firing rate, reported as a z-score of the average, pre-stimulus firing rate, of all RS neurons recorded by the Neuropixels probes targeting the stimulated cortex (MO) and SM-TH. Reproduced from \u201cCortico-thalamo-cortical interactions modulate electrically evoked EEG responses in mice\u201d."]})}),"\n",(0,a.jsx)(t.h2,{id:"a-new-perspective-zooming-in-on-synaptic-potentials",children:"A New Perspective: Zooming in on Synaptic Potentials"}),"\n",(0,a.jsxs)(t.p,{children:["Just a few months later, in November 2023, Drs. Richard Burman, Paul Brodersen and colleagues at the University of Oxford wrote a paper that included reanalysis of this data.\nTheir findings were published in the journal ",(0,a.jsx)(t.em,{children:"Neuron"})," with the article, ",(0,a.jsxs)(t.a,{href:"https://doi.org/10.1016/j.neuron.2023.08.005",children:["\u201cActive cortical networks promote shunting fast synaptic inhibition ",(0,a.jsx)(t.em,{children:"in vivo"}),"\u201d"]}),".\nThey also investigated the effects of anesthesia on brain activity, but narrowed their focus to the differential function of a specific receptor subtype: ",(0,a.jsxs)(t.a,{href:"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2648504/",children:["GABA",(0,a.jsx)("sub",{children:"A"}),"R"]}),"."]}),"\n",(0,a.jsxs)(t.p,{children:["Burman and Brodersen's team used a technique called ",(0,a.jsxs)(t.a,{href:"https://doi.org/10.1254/fpj.113.339",children:[(0,a.jsx)(t.em,{children:"in vivo"})," gramicidin perforated patch-clamp recording"]}),", combined with ",(0,a.jsx)(t.a,{href:"https://doi.org/10.1097/ICU.0000000000000140",children:"optogenetic"})," activation of GABAergic synaptic inputs.\nThey compared the GABA",(0,a.jsx)("sub",{children:"A"})," receptor equilibrium potential (E",(0,a.jsxs)("sub",{children:["GABA",(0,a.jsx)("sub",{children:"A"}),"R"]}),") in mice during wakefulness and under urethane anesthesia."]}),"\n",(0,a.jsxs)(t.p,{children:["Their findings were striking. In anesthetized mice, GABA",(0,a.jsx)("sub",{children:"A"}),' receptor responses were significantly hyperpolarizing, with an average equilibrium potential of -80.8mV.\nIn awake mice, however, these responses could be either hyperpolarizing or depolarizing, averaging at -63.9mV \u2013 a state known as the "',(0,a.jsx)(t.a,{href:"https://en.wikipedia.org/wiki/Shunting_inhibition",children:"shunting"}),'" regime.']}),"\n",(0,a.jsxs)(t.p,{children:["Burman et al. hypothesized that the higher network activity in the awake state leads to a higher concentration of intracellular chloride, which in turn depolarizes the GABA",(0,a.jsx)("sub",{children:"A"})," receptor equilibrium potential.\nAnesthesia, by broadly suppressing neural activity, indirectly hyperpolarizes E",(0,a.jsxs)("sub",{children:["GABA",(0,a.jsx)("sub",{children:"A"}),"R"]}),".\nThey confirmed this hypothesis by locally injecting an AMPA receptor antagonist (",(0,a.jsx)(t.a,{href:"https://www.tocris.com/products/nbqx-disodium-salt_1044",children:"NBQX"}),") which suppressed network activity, and hyperpolarized E",(0,a.jsxs)("sub",{children:["GABA",(0,a.jsx)("sub",{children:"A"}),"R"]})," to -78.0mV."]}),"\n",(0,a.jsxs)(t.p,{children:["The Oxford researchers also hypothesized that changes in E",(0,a.jsxs)("sub",{children:["GABA",(0,a.jsx)("sub",{children:"A"}),"R"]})," would lead to changes in network activity, resulting in a bi-directional relationship between the two quantities."]}),"\n",(0,a.jsxs)(t.p,{children:["To test this hypothesis, Burman and Brodersen's team turned to the data from ",(0,a.jsx)(t.a,{href:"https://dandiarchive.org/dandiset/000458",children:"Dandiset #000458"})," \u2013 the very same dataset used by Claar et al.\nBy reanalyzing this data and manipulating a computational network model, they found that setting the E",(0,a.jsxs)("sub",{children:["GABA",(0,a.jsx)("sub",{children:"A"}),"R"]})," parameter to the shunting regime (-60mV) reproduced spiking responses similar to those seen in awake mice (Figure 2 B&F left).\nConversely, setting it to the hyperpolarizing regime (-80mV) mimicked the responses seen in anesthetized mice (Figure 2 B&F right).\nThis data provides evidence for the hypothesized bidirectional relationship."]}),"\n",(0,a.jsx)(t.p,{children:(0,a.jsx)(t.img,{alt:"Figure2",src:n(4059).A+"",width:"1500",height:"582"})}),"\n",(0,a.jsx)(t.p,{children:(0,a.jsxs)(t.em,{children:[(0,a.jsx)(t.strong,{children:"Figure 2: Shunting inhibition promotes local network desynchronization and response flexibility."}),"\n(A) High-density Neuropixels (NPXs) recordings were used to compare spiking activity in the same cortical neurons under awake and anesthetized conditions.\n(B) Example raster plots show the spiking activity of a population of neurons in somatosensory cortex (SS). Spiking activity is shown across three stimulation trials for each condition.\n(C) Neuronal synchrony on a trial-to-trial basis (top) and the entropy of the peri-stimulus histogram (bottom) were calculated for each of the 662 recorded neurons under awake and anesthetized conditions (17 probe recordings in 16 mice). Each dot corresponds to a single neuron and the dashed line indicates the line of equality.\n(D) Neurons in the awake condition exhibited lower synchrony (top; Aw.: 0.325 \xb1 0.005 vs. An.: 0.393 \xb1 0.007, p < 0.001, paired t test) and higher entropy (bottom; Aw.: 1.347 \xb1 0.018 nats vs. An.: 1.111 \xb1 0.020 nats, p < 0.001, paired t test).\n(E) Schematic of network model consisting of interconnected excitatory pyramidal neurons (Pyr.) and inhibitory interneurons (IN). EGABAAR in the pyramidal neurons was adjusted relative to the RMP to create two different conditions: a shunting (Shunt.) and a hyperpolarizing (Hyperpol.) EGABAAR condition. Spiking activity was evoked by delivering brief depolarizing currents (input) of varying amplitudes to each neuron in the network.\n(F) Raster plots for the same population of pyramidal neurons (n = 50) in the shunting (left) and hyperpolarizing (right) EGABAAR conditions.\n(G) Synchrony (top) and entropy (bottom) for pyramidal neurons in the shunting and hyperpolarizing EGABAAR conditions (n = 1,000 randomly selected). Dashed line indicates the line of equality. (H) Neurons in the shunting EGABAAR condition exhibited lower synchrony (Shunt: 0.784 \xb1 0.001 vs. Hyperpol: 0.881 \xb1 0.001, p < 0.001, paired t test) and higher entropy (Shunt: 0.451 \xb1 0.002 nats vs. Hyperpol: 0.197 \xb1 0.002 nats, p < 0.001, paired t test) (n = 16,000 pyramidal neurons from the model). \u2217\u2217\u2217p < 0.001. MO, motor cortex; Stim., electrical stimulation; TH, thalamus. Reproduced from \u201cActive cortical networks promote shunting fast synaptic inhibition in vivo\u201d."]})}),"\n",(0,a.jsx)(t.h2,{id:"bridging-scales-from-synapses-to-networks",children:"Bridging Scales: From Synapses to Networks"}),"\n",(0,a.jsxs)(t.p,{children:["Together, these two studies provide a rich, multi-level account of what happens to brain activity under anesthesia. They span from macroscale brain signals recorded by EEG all the way down to microscale synaptic responses from a single receptor type.\nThe population spiking data from ",(0,a.jsx)(t.a,{href:"https://dandiarchive.org/dandiset/000458",children:"Dandiset #000458"})," serves as a crucial bridge between these levels, allowing Burman et al. to explicitly connect their work to the broader field of anesthesia research."]}),"\n",(0,a.jsx)(t.p,{children:"However, it's important to note some key differences between the two studies. Claar et al. used isoflurane anesthesia and stimulated layers 5/6 of the cortex, while Burman et al. used urethane anesthesia and recorded from layer 2/3.\nAdditionally, while Claar et al. found the most dramatic differences between waking and anesthetized states around 180 ms after stimulation, Burman et al. focused their reanalysis on the initial spiking response within 15 ms of stimulation."}),"\n",(0,a.jsxs)(t.p,{children:["These differences highlight the potential for further reanalysis of ",(0,a.jsx)(t.a,{href:"https://dandiarchive.org/dandiset/000458",children:"Dandiset #000458"}),".\nFuture studies could explore more similar experimental conditions, conduct a more thorough treatment of relevant response time periods, or investigate different aspects of anesthesia's effects altogether."]}),"\n",(0,a.jsx)(t.h2,{id:"engaging-with-the-open-science-community",children:"Engaging with the Open Science Community"}),"\n",(0,a.jsx)(t.p,{children:"For those inspired by the work of Claar, Burman and Brodersen, and their teams, the journey doesn\u2019t end here.\nWe encourage readers from all backgrounds to take advantage of this open science platform by publishing and reanalyzing data on DANDI.\nWe are confident that it will be a smooth and rewarding experience, but don't just take our word for it -- listen to what Dr. Leslie Claar and Dr. Paul Brodersen have to say,"}),"\n",(0,a.jsxs)(t.blockquote,{children:["\n",(0,a.jsx)(t.p,{children:'"After organizing our dataset into NWB format, validating and uploading our files was straightforward using a few simple commands from the DANDI Python Client.\nThe tools are well-documented, which made the process of uploading and publishing quite smooth.\nWithin a few months of publishing on DANDI, others were already interested in using our dataset."\n-- Dr. Leslie Claar'}),"\n"]}),"\n",(0,a.jsxs)(t.blockquote,{children:["\n",(0,a.jsx)(t.p,{children:"\u201cThe DANDI archive is a fantastic resource with great tooling and exhaustive documentation. Its command line interface makes data retrieval and synchronization as simple as possible (but not simpler!).\nThe standardized and well-documented formats for electrophysiological and neuroimaging data ensure that anyone can quickly find their way around a new archive without requiring extensive feedback from the authors that originally collected the data.\nThese features make the DANDI archive a treasure trove of useful and reusable data, unlike the unfortunately still commonplace unstructured data dumps on many other platforms or -- worst of all -- university servers.\u201d\n-- Dr. Paul Brodersen and Professor Colin Akerman"}),"\n"]}),"\n",(0,a.jsxs)(t.p,{children:["To get you started on your own reanalysis project, we've prepared a Jupyter ",(0,a.jsx)(t.a,{href:"https://github.com/dandi/example-notebooks/blob/master/000458/AllenInstitute/reanalysis.ipynb",children:"notebook"})," demonstrating some basic reanalysis of ",(0,a.jsx)(t.a,{href:"https://dandiarchive.org/dandiset/000458",children:"Dandiset #000458"}),".\nThis tool will help you dive right into the data, guiding you through the initial steps of exploration and analysis.\nWe have also prepared a Neurosift ",(0,a.jsx)(t.a,{href:"https://github.com/dandi/example-notebooks/blob/master/000458/FlatironInstitute/PSTH_neurosift_exploration.md",children:"walkthrough"})," which demonstrates how to use Neurosift to quickly and easily explore the NWB files in this dataset without even leaving the browser."]}),"\n",(0,a.jsx)(t.p,{children:"By engaging with this open science platform, you become part of a larger community of researchers who are building on each other's work and driving the field forward. In the world of open science, collaboration and curiosity drive discovery\u2014so why not see where the data takes you?"})]})}function c(e={}){const{wrapper:t}={...(0,s.R)(),...e.components};return t?(0,a.jsx)(t,{...e,children:(0,a.jsx)(d,{...e})}):d(e)}},4059:(e,t,n)=>{n.d(t,{A:()=>i});const i=n.p+"assets/images/data_reuse_000458_elife-fig7-14d309a15c91768081366dc94ab7d5d7.jpg"},8453:(e,t,n)=>{n.d(t,{R:()=>r,x:()=>o});var i=n(6540);const a={},s=i.createContext(a);function r(e){const t=i.useContext(s);return i.useMemo((function(){return"function"==typeof e?e(t):{...t,...e}}),[t,e])}function o(e){let t;return t=e.disableParentContext?"function"==typeof e.components?e.components(a):e.components||a:r(e.components),i.createElement(s.Provider,{value:t},e.children)}},5848:e=>{e.exports=JSON.parse('{"permalink":"/dandi-docusaurus-example/blog/2024/09/09/data_reuse_000458","editUrl":"https://github.com/facebook/docusaurus/tree/main/packages/create-docusaurus/templates/shared/blog/2024-09-09-data_reuse_000458.md","source":"@site/blog/2024-09-09-data_reuse_000458.md","title":"Unraveling Anesthesia\'s Effect on Brain Activity","description":"Data Reuse Highlight: This blog entry is part of an ongoing series that showcases examples of data reuse and reanalysis using publicly available data on DANDI.","date":"2024-09-09T00:00:00.000Z","tags":[{"inline":true,"label":"reuse showcase","permalink":"/dandi-docusaurus-example/blog/tags/reuse-showcase"}],"readingTime":8.45,"hasTruncateMarker":false,"authors":[{"name":"Paul Adkisson","key":null,"page":null}],"frontMatter":{"title":"Unraveling Anesthesia\'s Effect on Brain Activity","author":"Paul Adkisson","tags":["reuse showcase"]},"unlisted":false,"nextItem":{"title":"First Blog Post","permalink":"/dandi-docusaurus-example/blog/first-blog-post"}}')}}]); \ No newline at end of file +"use strict";(self.webpackChunkdandi_website=self.webpackChunkdandi_website||[]).push([[110],{8544:(e,t,n)=>{n.r(t),n.d(t,{assets:()=>l,contentTitle:()=>o,default:()=>c,frontMatter:()=>r,metadata:()=>i,toc:()=>h});var i=n(5848),a=n(4848),s=n(8453);const r={title:"Unraveling Anesthesia's Effect on Brain Activity",author:"Paul Adkisson",tags:["reuse showcase"]},o=void 0,l={authorsImageUrls:[void 0]},h=[{value:"The Original Study: Peering into the Anesthetized Brain",id:"the-original-study-peering-into-the-anesthetized-brain",level:2},{value:"A New Perspective: Zooming in on Synaptic Potentials",id:"a-new-perspective-zooming-in-on-synaptic-potentials",level:2},{value:"Bridging Scales: From Synapses to Networks",id:"bridging-scales-from-synapses-to-networks",level:2},{value:"Engaging with the Open Science Community",id:"engaging-with-the-open-science-community",level:2}];function d(e){const t={a:"a",blockquote:"blockquote",em:"em",h2:"h2",img:"img",p:"p",strong:"strong",...(0,s.R)(),...e.components};return(0,a.jsxs)(a.Fragment,{children:[(0,a.jsx)(t.p,{children:(0,a.jsx)(t.em,{children:"Data Reuse Highlight: This blog entry is part of an ongoing series that showcases examples of data reuse and reanalysis using publicly available data on DANDI."})}),"\n",(0,a.jsxs)(t.p,{children:["Have you ever wondered what happens to your brain when you're under anesthesia? What neural changes coincide with that dramatic loss of consciousness?\nIn this post, we'll explore how two different research teams investigated this fascinating question using the same data from DANDI Archive's ",(0,a.jsx)(t.a,{href:"https://dandiarchive.org/dandiset/000458",children:"Dandiset #000458"}),"."]}),"\n",(0,a.jsx)(t.h2,{id:"the-original-study-peering-into-the-anesthetized-brain",children:"The Original Study: Peering into the Anesthetized Brain"}),"\n",(0,a.jsxs)(t.p,{children:["In June 2023, Dr. Leslie Claar and colleagues from the Allen Institute published their groundbreaking findings in the journal ",(0,a.jsx)(t.em,{children:"eLife"})," in the article, ",(0,a.jsx)(t.a,{href:"https://doi.org/10.7554/eLife.84630.3",children:"\u201cCortico-thalamo-cortical interactions modulate electrically evoked EEG responses in mice\u201d"}),".\nTheir approach was comprehensive: they stimulated mouse cortex while simultaneously recording with ",(0,a.jsx)(t.a,{href:"https://doi.org/10.1016/B978-141603618-0.10024-4",children:"electroencephelography"})," (EEG) and ",(0,a.jsx)(t.a,{href:"https://www.neuropixels.org/",children:"neuropixels probes"}),", comparing brain activity during wakefulness and under isoflurane anesthesia."]}),"\n",(0,a.jsxs)(t.p,{children:["In awake mice, Claar's team observed a robust ",(0,a.jsx)(t.a,{href:"https://doi.org/10.4103/0972-6748.57865",children:"event-related potential"})," (ERP) when they delivered pulsatile electrical stimulation to deep layers of the ",(0,a.jsx)(t.a,{href:"https://doi.org/10.1016/B0-08-043076-7/03460-4",children:"motor cortex"})," (Figure 1A left).\nThis ERP coincided with a distinct pattern of neural spiking, both locally in the motor cortex and in distant sensorimotor-related ",(0,a.jsx)(t.a,{href:"https://www.ncbi.nlm.nih.gov/books/NBK549908",children:"thalamic nuclei"})," (Figure 1B left). The response followed a three-act play: initial excitation within 25 milliseconds, a period of quiescence for about 125 milliseconds, and finally, a strong rebound excitation before returning to baseline.\nWhen the mice were under anesthesia, the story changed dramatically.\nThe ERP's amplitude and complexity plummeted in response to the same stimulation (Figure 1A right).\nWhile the initial excitation persisted in the motor cortex, it vanished in the thalamic nuclei (Figure 1B right).\nThe rebound excitation, along with its corresponding ERP component at around 180 milliseconds, was significantly weakened."]}),"\n",(0,a.jsx)(t.p,{children:"Based on these observations, Claar and colleagues proposed that interactions between the cortex and thalamus are responsible for the rebound excitation phase of the stimulus response.\nThis rebound excitation, in turn, drives the second component of the ERP \u2013 a crucial signal of wakefulness."}),"\n",(0,a.jsx)(t.p,{children:"After their experiments, Claar and her team published their data on DANDI, ensuring that it would remain publicly accessible for any future researchers who wanted to use it. The dataset and manuscript were prepared and published concurrently, which ensured that the dataset included all the paper-relevant data and metadata and curious reviewers could inspect the data directly if they so wished."}),"\n",(0,a.jsx)(t.p,{children:(0,a.jsx)(t.img,{alt:"Figure1",src:n(4059).A+"",width:"1500",height:"582"})}),"\n",(0,a.jsx)(t.p,{children:(0,a.jsxs)(t.em,{children:[(0,a.jsx)(t.strong,{children:"Figure 1: Brain state modulates the ERP via cortico-thalamo-cortical interactions."}),"\n(A) Butterfly plot of ERPs during non-running (quiet wakefulness), running (active wakefulness), and isoflurane-anesthetized states.\n(B) Normalized firing rate, reported as a z-score of the average, pre-stimulus firing rate, of all RS neurons recorded by the Neuropixels probes targeting the stimulated cortex (MO) and SM-TH. Reproduced from \u201cCortico-thalamo-cortical interactions modulate electrically evoked EEG responses in mice\u201d."]})}),"\n",(0,a.jsx)(t.h2,{id:"a-new-perspective-zooming-in-on-synaptic-potentials",children:"A New Perspective: Zooming in on Synaptic Potentials"}),"\n",(0,a.jsxs)(t.p,{children:["Just a few months later, in November 2023, Drs. Richard Burman, Paul Brodersen and colleagues at the University of Oxford wrote a paper that included reanalysis of this data.\nTheir findings were published in the journal ",(0,a.jsx)(t.em,{children:"Neuron"})," with the article, ",(0,a.jsxs)(t.a,{href:"https://doi.org/10.1016/j.neuron.2023.08.005",children:["\u201cActive cortical networks promote shunting fast synaptic inhibition ",(0,a.jsx)(t.em,{children:"in vivo"}),"\u201d"]}),".\nThey also investigated the effects of anesthesia on brain activity, but narrowed their focus to the differential function of a specific receptor subtype: ",(0,a.jsxs)(t.a,{href:"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2648504/",children:["GABA",(0,a.jsx)("sub",{children:"A"}),"R"]}),"."]}),"\n",(0,a.jsxs)(t.p,{children:["Burman and Brodersen's team used a technique called ",(0,a.jsxs)(t.a,{href:"https://doi.org/10.1254/fpj.113.339",children:[(0,a.jsx)(t.em,{children:"in vivo"})," gramicidin perforated patch-clamp recording"]}),", combined with ",(0,a.jsx)(t.a,{href:"https://doi.org/10.1097/ICU.0000000000000140",children:"optogenetic"})," activation of GABAergic synaptic inputs.\nThey compared the GABA",(0,a.jsx)("sub",{children:"A"})," receptor equilibrium potential (E",(0,a.jsxs)("sub",{children:["GABA",(0,a.jsx)("sub",{children:"A"}),"R"]}),") in mice during wakefulness and under urethane anesthesia."]}),"\n",(0,a.jsxs)(t.p,{children:["Their findings were striking. In anesthetized mice, GABA",(0,a.jsx)("sub",{children:"A"}),' receptor responses were significantly hyperpolarizing, with an average equilibrium potential of -80.8mV.\nIn awake mice, however, these responses could be either hyperpolarizing or depolarizing, averaging at -63.9mV \u2013 a state known as the "',(0,a.jsx)(t.a,{href:"https://en.wikipedia.org/wiki/Shunting_inhibition",children:"shunting"}),'" regime.']}),"\n",(0,a.jsxs)(t.p,{children:["Burman et al. hypothesized that the higher network activity in the awake state leads to a higher concentration of intracellular chloride, which in turn depolarizes the GABA",(0,a.jsx)("sub",{children:"A"})," receptor equilibrium potential.\nAnesthesia, by broadly suppressing neural activity, indirectly hyperpolarizes E",(0,a.jsxs)("sub",{children:["GABA",(0,a.jsx)("sub",{children:"A"}),"R"]}),".\nThey confirmed this hypothesis by locally injecting an AMPA receptor antagonist (",(0,a.jsx)(t.a,{href:"https://www.tocris.com/products/nbqx-disodium-salt_1044",children:"NBQX"}),") which suppressed network activity, and hyperpolarized E",(0,a.jsxs)("sub",{children:["GABA",(0,a.jsx)("sub",{children:"A"}),"R"]})," to -78.0mV."]}),"\n",(0,a.jsxs)(t.p,{children:["The Oxford researchers also hypothesized that changes in E",(0,a.jsxs)("sub",{children:["GABA",(0,a.jsx)("sub",{children:"A"}),"R"]})," would lead to changes in network activity, resulting in a bi-directional relationship between the two quantities."]}),"\n",(0,a.jsxs)(t.p,{children:["To test this hypothesis, Burman and Brodersen's team turned to the data from ",(0,a.jsx)(t.a,{href:"https://dandiarchive.org/dandiset/000458",children:"Dandiset #000458"})," \u2013 the very same dataset used by Claar et al.\nBy reanalyzing this data and manipulating a computational network model, they found that setting the E",(0,a.jsxs)("sub",{children:["GABA",(0,a.jsx)("sub",{children:"A"}),"R"]})," parameter to the shunting regime (-60mV) reproduced spiking responses similar to those seen in awake mice (Figure 2 B&F left).\nConversely, setting it to the hyperpolarizing regime (-80mV) mimicked the responses seen in anesthetized mice (Figure 2 B&F right).\nThis data provides evidence for the hypothesized bidirectional relationship."]}),"\n",(0,a.jsx)(t.p,{children:(0,a.jsx)(t.img,{alt:"Figure2",src:n(7287).A+"",width:"3000",height:"2091"})}),"\n",(0,a.jsx)(t.p,{children:(0,a.jsxs)(t.em,{children:[(0,a.jsx)(t.strong,{children:"Figure 2: Shunting inhibition promotes local network desynchronization and response flexibility."}),"\n(A) High-density Neuropixels (NPXs) recordings were used to compare spiking activity in the same cortical neurons under awake and anesthetized conditions.\n(B) Example raster plots show the spiking activity of a population of neurons in somatosensory cortex (SS). Spiking activity is shown across three stimulation trials for each condition.\n(C) Neuronal synchrony on a trial-to-trial basis (top) and the entropy of the peri-stimulus histogram (bottom) were calculated for each of the 662 recorded neurons under awake and anesthetized conditions (17 probe recordings in 16 mice). Each dot corresponds to a single neuron and the dashed line indicates the line of equality.\n(D) Neurons in the awake condition exhibited lower synchrony (top; Aw.: 0.325 \xb1 0.005 vs. An.: 0.393 \xb1 0.007, p < 0.001, paired t test) and higher entropy (bottom; Aw.: 1.347 \xb1 0.018 nats vs. An.: 1.111 \xb1 0.020 nats, p < 0.001, paired t test).\n(E) Schematic of network model consisting of interconnected excitatory pyramidal neurons (Pyr.) and inhibitory interneurons (IN). EGABAAR in the pyramidal neurons was adjusted relative to the RMP to create two different conditions: a shunting (Shunt.) and a hyperpolarizing (Hyperpol.) EGABAAR condition. Spiking activity was evoked by delivering brief depolarizing currents (input) of varying amplitudes to each neuron in the network.\n(F) Raster plots for the same population of pyramidal neurons (n = 50) in the shunting (left) and hyperpolarizing (right) EGABAAR conditions.\n(G) Synchrony (top) and entropy (bottom) for pyramidal neurons in the shunting and hyperpolarizing EGABAAR conditions (n = 1,000 randomly selected). Dashed line indicates the line of equality. (H) Neurons in the shunting EGABAAR condition exhibited lower synchrony (Shunt: 0.784 \xb1 0.001 vs. Hyperpol: 0.881 \xb1 0.001, p < 0.001, paired t test) and higher entropy (Shunt: 0.451 \xb1 0.002 nats vs. Hyperpol: 0.197 \xb1 0.002 nats, p < 0.001, paired t test) (n = 16,000 pyramidal neurons from the model). \u2217\u2217\u2217p < 0.001. MO, motor cortex; Stim., electrical stimulation; TH, thalamus. Reproduced from \u201cActive cortical networks promote shunting fast synaptic inhibition in vivo\u201d."]})}),"\n",(0,a.jsx)(t.h2,{id:"bridging-scales-from-synapses-to-networks",children:"Bridging Scales: From Synapses to Networks"}),"\n",(0,a.jsxs)(t.p,{children:["Together, these two studies provide a rich, multi-level account of what happens to brain activity under anesthesia. They span from macroscale brain signals recorded by EEG all the way down to microscale synaptic responses from a single receptor type.\nThe population spiking data from ",(0,a.jsx)(t.a,{href:"https://dandiarchive.org/dandiset/000458",children:"Dandiset #000458"})," serves as a crucial bridge between these levels, allowing Burman et al. to explicitly connect their work to the broader field of anesthesia research."]}),"\n",(0,a.jsx)(t.p,{children:"However, it's important to note some key differences between the two studies. Claar et al. used isoflurane anesthesia and stimulated layers 5/6 of the cortex, while Burman et al. used urethane anesthesia and recorded from layer 2/3.\nAdditionally, while Claar et al. found the most dramatic differences between waking and anesthetized states around 180 ms after stimulation, Burman et al. focused their reanalysis on the initial spiking response within 15 ms of stimulation."}),"\n",(0,a.jsxs)(t.p,{children:["These differences highlight the potential for further reanalysis of ",(0,a.jsx)(t.a,{href:"https://dandiarchive.org/dandiset/000458",children:"Dandiset #000458"}),".\nFuture studies could explore more similar experimental conditions, conduct a more thorough treatment of relevant response time periods, or investigate different aspects of anesthesia's effects altogether."]}),"\n",(0,a.jsx)(t.h2,{id:"engaging-with-the-open-science-community",children:"Engaging with the Open Science Community"}),"\n",(0,a.jsx)(t.p,{children:"For those inspired by the work of Claar, Burman and Brodersen, and their teams, the journey doesn\u2019t end here.\nWe encourage readers from all backgrounds to take advantage of this open science platform by publishing and reanalyzing data on DANDI.\nWe are confident that it will be a smooth and rewarding experience, but don't just take our word for it -- listen to what Dr. Leslie Claar and Dr. Paul Brodersen have to say,"}),"\n",(0,a.jsxs)(t.blockquote,{children:["\n",(0,a.jsx)(t.p,{children:'"After organizing our dataset into NWB format, validating and uploading our files was straightforward using a few simple commands from the DANDI Python Client.\nThe tools are well-documented, which made the process of uploading and publishing quite smooth.\nWithin a few months of publishing on DANDI, others were already interested in using our dataset."\n-- Dr. Leslie Claar'}),"\n"]}),"\n",(0,a.jsxs)(t.blockquote,{children:["\n",(0,a.jsx)(t.p,{children:"\u201cThe DANDI archive is a fantastic resource with great tooling and exhaustive documentation. Its command line interface makes data retrieval and synchronization as simple as possible (but not simpler!).\nThe standardized and well-documented formats for electrophysiological and neuroimaging data ensure that anyone can quickly find their way around a new archive without requiring extensive feedback from the authors that originally collected the data.\nThese features make the DANDI archive a treasure trove of useful and reusable data, unlike the unfortunately still commonplace unstructured data dumps on many other platforms or -- worst of all -- university servers.\u201d\n-- Dr. Paul Brodersen and Professor Colin Akerman"}),"\n"]}),"\n",(0,a.jsxs)(t.p,{children:["To get you started on your own reanalysis project, we've prepared a Jupyter ",(0,a.jsx)(t.a,{href:"https://github.com/dandi/example-notebooks/blob/master/000458/AllenInstitute/reanalysis.ipynb",children:"notebook"})," demonstrating some basic reanalysis of ",(0,a.jsx)(t.a,{href:"https://dandiarchive.org/dandiset/000458",children:"Dandiset #000458"}),".\nThis tool will help you dive right into the data, guiding you through the initial steps of exploration and analysis.\nWe have also prepared a Neurosift ",(0,a.jsx)(t.a,{href:"https://github.com/dandi/example-notebooks/blob/master/000458/FlatironInstitute/PSTH_neurosift_exploration.md",children:"walkthrough"})," which demonstrates how to use Neurosift to quickly and easily explore the NWB files in this dataset without even leaving the browser."]}),"\n",(0,a.jsx)(t.p,{children:"By engaging with this open science platform, you become part of a larger community of researchers who are building on each other's work and driving the field forward. In the world of open science, collaboration and curiosity drive discovery\u2014so why not see where the data takes you?"})]})}function c(e={}){const{wrapper:t}={...(0,s.R)(),...e.components};return t?(0,a.jsx)(t,{...e,children:(0,a.jsx)(d,{...e})}):d(e)}},7287:(e,t,n)=>{n.d(t,{A:()=>i});const i=n.p+"assets/images/data_reuse_000458_cell-fig4-f46a368caff320b8e49aec5e7b1de701.jpg"},4059:(e,t,n)=>{n.d(t,{A:()=>i});const i=n.p+"assets/images/data_reuse_000458_elife-fig7-14d309a15c91768081366dc94ab7d5d7.jpg"},8453:(e,t,n)=>{n.d(t,{R:()=>r,x:()=>o});var i=n(6540);const a={},s=i.createContext(a);function r(e){const t=i.useContext(s);return i.useMemo((function(){return"function"==typeof e?e(t):{...t,...e}}),[t,e])}function o(e){let t;return t=e.disableParentContext?"function"==typeof e.components?e.components(a):e.components||a:r(e.components),i.createElement(s.Provider,{value:t},e.children)}},5848:e=>{e.exports=JSON.parse('{"permalink":"/dandi-docusaurus-example/blog/2024/09/09/data_reuse_000458","editUrl":"https://github.com/facebook/docusaurus/tree/main/packages/create-docusaurus/templates/shared/blog/2024-09-09-data_reuse_000458.md","source":"@site/blog/2024-09-09-data_reuse_000458.md","title":"Unraveling Anesthesia\'s Effect on Brain Activity","description":"Data Reuse Highlight: This blog entry is part of an ongoing series that showcases examples of data reuse and reanalysis using publicly available data on DANDI.","date":"2024-09-09T00:00:00.000Z","tags":[{"inline":true,"label":"reuse showcase","permalink":"/dandi-docusaurus-example/blog/tags/reuse-showcase"}],"readingTime":8.45,"hasTruncateMarker":false,"authors":[{"name":"Paul Adkisson","key":null,"page":null}],"frontMatter":{"title":"Unraveling Anesthesia\'s Effect on Brain Activity","author":"Paul Adkisson","tags":["reuse showcase"]},"unlisted":false,"nextItem":{"title":"First Blog Post","permalink":"/dandi-docusaurus-example/blog/first-blog-post"}}')}}]); \ No newline at end of file diff --git a/assets/js/cb43e9f2.d6ecb7d8.js b/assets/js/cb43e9f2.d6ecb7d8.js new file mode 100644 index 0000000..e91e964 --- /dev/null +++ b/assets/js/cb43e9f2.d6ecb7d8.js @@ -0,0 +1 @@ +"use strict";(self.webpackChunkdandi_website=self.webpackChunkdandi_website||[]).push([[293],{1565:e=>{e.exports=JSON.parse('{"archive":{"blogPosts":[{"id":"/2024/09/09/data_reuse_000458","metadata":{"permalink":"/dandi-docusaurus-example/blog/2024/09/09/data_reuse_000458","editUrl":"https://github.com/facebook/docusaurus/tree/main/packages/create-docusaurus/templates/shared/blog/2024-09-09-data_reuse_000458.md","source":"@site/blog/2024-09-09-data_reuse_000458.md","title":"Unraveling Anesthesia\'s Effect on Brain Activity","description":"Data Reuse Highlight: This blog entry is part of an ongoing series that showcases examples of data reuse and reanalysis using publicly available data on DANDI.","date":"2024-09-09T00:00:00.000Z","tags":[{"inline":true,"label":"reuse showcase","permalink":"/dandi-docusaurus-example/blog/tags/reuse-showcase"}],"readingTime":8.45,"hasTruncateMarker":false,"authors":[{"name":"Paul Adkisson","key":null,"page":null}],"frontMatter":{"title":"Unraveling Anesthesia\'s Effect on Brain Activity","author":"Paul Adkisson","tags":["reuse showcase"]},"unlisted":false,"nextItem":{"title":"First Blog Post","permalink":"/dandi-docusaurus-example/blog/first-blog-post"}},"content":"_Data Reuse Highlight: This blog entry is part of an ongoing series that showcases examples of data reuse and reanalysis using publicly available data on DANDI._\\n\\nHave you ever wondered what happens to your brain when you\'re under anesthesia? What neural changes coincide with that dramatic loss of consciousness?\\nIn this post, we\'ll explore how two different research teams investigated this fascinating question using the same data from DANDI Archive\'s [Dandiset #000458](https://dandiarchive.org/dandiset/000458).\\n\\n## The Original Study: Peering into the Anesthetized Brain\\nIn June 2023, Dr. Leslie Claar and colleagues from the Allen Institute published their groundbreaking findings in the journal _eLife_ in the article, [\u201cCortico-thalamo-cortical interactions modulate electrically evoked EEG responses in mice\u201d](https://doi.org/10.7554/eLife.84630.3).\\nTheir approach was comprehensive: they stimulated mouse cortex while simultaneously recording with [electroencephelography](https://doi.org/10.1016/B978-141603618-0.10024-4) (EEG) and [neuropixels probes](https://www.neuropixels.org/), comparing brain activity during wakefulness and under isoflurane anesthesia.\\n\\nIn awake mice, Claar\'s team observed a robust [event-related potential](https://doi.org/10.4103/0972-6748.57865) (ERP) when they delivered pulsatile electrical stimulation to deep layers of the [motor cortex](https://doi.org/10.1016/B0-08-043076-7/03460-4) (Figure 1A left).\\nThis ERP coincided with a distinct pattern of neural spiking, both locally in the motor cortex and in distant sensorimotor-related [thalamic nuclei](https://www.ncbi.nlm.nih.gov/books/NBK549908) (Figure 1B left). The response followed a three-act play: initial excitation within 25 milliseconds, a period of quiescence for about 125 milliseconds, and finally, a strong rebound excitation before returning to baseline.\\nWhen the mice were under anesthesia, the story changed dramatically.\\nThe ERP\'s amplitude and complexity plummeted in response to the same stimulation (Figure 1A right).\\nWhile the initial excitation persisted in the motor cortex, it vanished in the thalamic nuclei (Figure 1B right).\\nThe rebound excitation, along with its corresponding ERP component at around 180 milliseconds, was significantly weakened.\\n\\nBased on these observations, Claar and colleagues proposed that interactions between the cortex and thalamus are responsible for the rebound excitation phase of the stimulus response.\\nThis rebound excitation, in turn, drives the second component of the ERP \u2013 a crucial signal of wakefulness.\\n\\nAfter their experiments, Claar and her team published their data on DANDI, ensuring that it would remain publicly accessible for any future researchers who wanted to use it. The dataset and manuscript were prepared and published concurrently, which ensured that the dataset included all the paper-relevant data and metadata and curious reviewers could inspect the data directly if they so wished.\\n\\n![Figure1](/img/blog/data_reuse_000458_elife-fig7.jpg)\\n\\n_**Figure 1: Brain state modulates the ERP via cortico-thalamo-cortical interactions.**\\n(A) Butterfly plot of ERPs during non-running (quiet wakefulness), running (active wakefulness), and isoflurane-anesthetized states.\\n(B) Normalized firing rate, reported as a z-score of the average, pre-stimulus firing rate, of all RS neurons recorded by the Neuropixels probes targeting the stimulated cortex (MO) and SM-TH. Reproduced from \u201cCortico-thalamo-cortical interactions modulate electrically evoked EEG responses in mice\u201d._\\n\\n## A New Perspective: Zooming in on Synaptic Potentials\\nJust a few months later, in November 2023, Drs. Richard Burman, Paul Brodersen and colleagues at the University of Oxford wrote a paper that included reanalysis of this data.\\nTheir findings were published in the journal _Neuron_ with the article, [\u201cActive cortical networks promote shunting fast synaptic inhibition _in vivo_\u201d](https://doi.org/10.1016/j.neuron.2023.08.005).\\nThey also investigated the effects of anesthesia on brain activity, but narrowed their focus to the differential function of a specific receptor subtype: [GABAAR](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2648504/).\\n\\nBurman and Brodersen\'s team used a technique called [_in vivo_ gramicidin perforated patch-clamp recording](https://doi.org/10.1254/fpj.113.339), combined with [optogenetic](https://doi.org/10.1097/ICU.0000000000000140) activation of GABAergic synaptic inputs.\\nThey compared the GABAA receptor equilibrium potential (EGABAAR) in mice during wakefulness and under urethane anesthesia.\\n\\nTheir findings were striking. In anesthetized mice, GABAA receptor responses were significantly hyperpolarizing, with an average equilibrium potential of -80.8mV.\\nIn awake mice, however, these responses could be either hyperpolarizing or depolarizing, averaging at -63.9mV \u2013 a state known as the \\"[shunting](https://en.wikipedia.org/wiki/Shunting_inhibition)\\" regime.\\n\\nBurman et al. hypothesized that the higher network activity in the awake state leads to a higher concentration of intracellular chloride, which in turn depolarizes the GABAA receptor equilibrium potential.\\nAnesthesia, by broadly suppressing neural activity, indirectly hyperpolarizes EGABAAR.\\nThey confirmed this hypothesis by locally injecting an AMPA receptor antagonist ([NBQX](https://www.tocris.com/products/nbqx-disodium-salt_1044)) which suppressed network activity, and hyperpolarized EGABAAR to -78.0mV.\\n\\nThe Oxford researchers also hypothesized that changes in EGABAAR would lead to changes in network activity, resulting in a bi-directional relationship between the two quantities.\\n\\nTo test this hypothesis, Burman and Brodersen\'s team turned to the data from [Dandiset #000458](https://dandiarchive.org/dandiset/000458) \u2013 the very same dataset used by Claar et al.\\nBy reanalyzing this data and manipulating a computational network model, they found that setting the EGABAAR parameter to the shunting regime (-60mV) reproduced spiking responses similar to those seen in awake mice (Figure 2 B&F left).\\nConversely, setting it to the hyperpolarizing regime (-80mV) mimicked the responses seen in anesthetized mice (Figure 2 B&F right).\\nThis data provides evidence for the hypothesized bidirectional relationship.\\n\\n![Figure2](/img/blog/data_reuse_000458_cell-fig4.jpg)\\n\\n_**Figure 2: Shunting inhibition promotes local network desynchronization and response flexibility.**\\n(A) High-density Neuropixels (NPXs) recordings were used to compare spiking activity in the same cortical neurons under awake and anesthetized conditions.\\n(B) Example raster plots show the spiking activity of a population of neurons in somatosensory cortex (SS). Spiking activity is shown across three stimulation trials for each condition.\\n(C) Neuronal synchrony on a trial-to-trial basis (top) and the entropy of the peri-stimulus histogram (bottom) were calculated for each of the 662 recorded neurons under awake and anesthetized conditions (17 probe recordings in 16 mice). Each dot corresponds to a single neuron and the dashed line indicates the line of equality.\\n(D) Neurons in the awake condition exhibited lower synchrony (top; Aw.: 0.325 \xb1 0.005 vs. An.: 0.393 \xb1 0.007, p < 0.001, paired t test) and higher entropy (bottom; Aw.: 1.347 \xb1 0.018 nats vs. An.: 1.111 \xb1 0.020 nats, p < 0.001, paired t test).\\n(E) Schematic of network model consisting of interconnected excitatory pyramidal neurons (Pyr.) and inhibitory interneurons (IN). EGABAAR in the pyramidal neurons was adjusted relative to the RMP to create two different conditions: a shunting (Shunt.) and a hyperpolarizing (Hyperpol.) EGABAAR condition. Spiking activity was evoked by delivering brief depolarizing currents (input) of varying amplitudes to each neuron in the network.\\n(F) Raster plots for the same population of pyramidal neurons (n = 50) in the shunting (left) and hyperpolarizing (right) EGABAAR conditions.\\n(G) Synchrony (top) and entropy (bottom) for pyramidal neurons in the shunting and hyperpolarizing EGABAAR conditions (n = 1,000 randomly selected). Dashed line indicates the line of equality. (H) Neurons in the shunting EGABAAR condition exhibited lower synchrony (Shunt: 0.784 \xb1 0.001 vs. Hyperpol: 0.881 \xb1 0.001, p < 0.001, paired t test) and higher entropy (Shunt: 0.451 \xb1 0.002 nats vs. Hyperpol: 0.197 \xb1 0.002 nats, p < 0.001, paired t test) (n = 16,000 pyramidal neurons from the model). \u2217\u2217\u2217p < 0.001. MO, motor cortex; Stim., electrical stimulation; TH, thalamus. Reproduced from \u201cActive cortical networks promote shunting fast synaptic inhibition in vivo\u201d._\\n\\n## Bridging Scales: From Synapses to Networks\\nTogether, these two studies provide a rich, multi-level account of what happens to brain activity under anesthesia. They span from macroscale brain signals recorded by EEG all the way down to microscale synaptic responses from a single receptor type.\\nThe population spiking data from [Dandiset #000458](https://dandiarchive.org/dandiset/000458) serves as a crucial bridge between these levels, allowing Burman et al. to explicitly connect their work to the broader field of anesthesia research.\\n\\nHowever, it\'s important to note some key differences between the two studies. Claar et al. used isoflurane anesthesia and stimulated layers 5/6 of the cortex, while Burman et al. used urethane anesthesia and recorded from layer 2/3.\\nAdditionally, while Claar et al. found the most dramatic differences between waking and anesthetized states around 180 ms after stimulation, Burman et al. focused their reanalysis on the initial spiking response within 15 ms of stimulation.\\n\\nThese differences highlight the potential for further reanalysis of [Dandiset #000458](https://dandiarchive.org/dandiset/000458).\\nFuture studies could explore more similar experimental conditions, conduct a more thorough treatment of relevant response time periods, or investigate different aspects of anesthesia\'s effects altogether.\\n\\n## Engaging with the Open Science Community\\nFor those inspired by the work of Claar, Burman and Brodersen, and their teams, the journey doesn\u2019t end here.\\nWe encourage readers from all backgrounds to take advantage of this open science platform by publishing and reanalyzing data on DANDI.\\nWe are confident that it will be a smooth and rewarding experience, but don\'t just take our word for it -- listen to what Dr. Leslie Claar and Dr. Paul Brodersen have to say,\\n\\n> \\"After organizing our dataset into NWB format, validating and uploading our files was straightforward using a few simple commands from the DANDI Python Client. \\n> The tools are well-documented, which made the process of uploading and publishing quite smooth. \\n> Within a few months of publishing on DANDI, others were already interested in using our dataset.\\"\\n> -- Dr. Leslie Claar\\n\\n> \u201cThe DANDI archive is a fantastic resource with great tooling and exhaustive documentation. Its command line interface makes data retrieval and synchronization as simple as possible (but not simpler!).\\n> The standardized and well-documented formats for electrophysiological and neuroimaging data ensure that anyone can quickly find their way around a new archive without requiring extensive feedback from the authors that originally collected the data.\\n> These features make the DANDI archive a treasure trove of useful and reusable data, unlike the unfortunately still commonplace unstructured data dumps on many other platforms or -- worst of all -- university servers.\u201d\\n> -- Dr. Paul Brodersen and Professor Colin Akerman\\n\\nTo get you started on your own reanalysis project, we\'ve prepared a Jupyter [notebook](https://github.com/dandi/example-notebooks/blob/master/000458/AllenInstitute/reanalysis.ipynb) demonstrating some basic reanalysis of [Dandiset #000458](https://dandiarchive.org/dandiset/000458).\\nThis tool will help you dive right into the data, guiding you through the initial steps of exploration and analysis.\\nWe have also prepared a Neurosift [walkthrough](https://github.com/dandi/example-notebooks/blob/master/000458/FlatironInstitute/PSTH_neurosift_exploration.md) which demonstrates how to use Neurosift to quickly and easily explore the NWB files in this dataset without even leaving the browser. \\n\\nBy engaging with this open science platform, you become part of a larger community of researchers who are building on each other\'s work and driving the field forward. In the world of open science, collaboration and curiosity drive discovery\u2014so why not see where the data takes you?"},{"id":"first-blog-post","metadata":{"permalink":"/dandi-docusaurus-example/blog/first-blog-post","editUrl":"https://github.com/facebook/docusaurus/tree/main/packages/create-docusaurus/templates/shared/blog/2019-05-28-first-blog-post.mdx","source":"@site/blog/2019-05-28-first-blog-post.mdx","title":"First Blog Post","description":"Lorem ipsum dolor sit amet...","date":"2019-05-28T00:00:00.000Z","tags":[{"inline":false,"label":"Hola","permalink":"/dandi-docusaurus-example/blog/tags/hola","description":"Hola tag description"},{"inline":false,"label":"Docusaurus","permalink":"/dandi-docusaurus-example/blog/tags/docusaurus","description":"Docusaurus tag description"}],"readingTime":0.395,"hasTruncateMarker":true,"authors":[{"name":"Luiz Tauffer","title":"CatalystNeuro","url":"https://github.com/luiztauffer","socials":{"github":"https://github.com/luiztauffer"},"imageURL":"https://avatars.githubusercontent.com/u/24541631?v=4","key":"luiztauffer","page":null},{"name":"Ben Dichter","title":"CatalystNeuro","url":"https://github.com/bendichter","socials":{"github":"https://github.com/bendichter"},"imageURL":"https://avatars.githubusercontent.com/u/844306?v=4","key":"bendichter","page":null}],"frontMatter":{"slug":"first-blog-post","title":"First Blog Post","authors":["luiztauffer","bendichter"],"tags":["hola","docusaurus"]},"unlisted":false,"prevItem":{"title":"Unraveling Anesthesia\'s Effect on Brain Activity","permalink":"/dandi-docusaurus-example/blog/2024/09/09/data_reuse_000458"}},"content":"Lorem ipsum dolor sit amet...\\n\\n{/* truncate */}\\n\\n\\n:::tip\\n\\nExploring data in Neurosift\\n\\n:::\\n\\n...consectetur adipiscing elit. Pellentesque elementum dignissim ultricies. Fusce rhoncus ipsum tempor eros aliquam consequat. Lorem ipsum dolor sit amet\\n\\n\\n\\n\\n\\n\\n\\nWe can add interactive components. For example, use JSX to create an interactive button:\\n\\n```js\\n\\n```\\n\\n"}]}}')}}]); \ No newline at end of file diff --git a/assets/js/cb43e9f2.f1c85078.js b/assets/js/cb43e9f2.f1c85078.js deleted file mode 100644 index 25ae0b1..0000000 --- a/assets/js/cb43e9f2.f1c85078.js +++ /dev/null @@ -1 +0,0 @@ -"use strict";(self.webpackChunkdandi_website=self.webpackChunkdandi_website||[]).push([[293],{1565:e=>{e.exports=JSON.parse('{"archive":{"blogPosts":[{"id":"/2024/09/09/data_reuse_000458","metadata":{"permalink":"/dandi-docusaurus-example/blog/2024/09/09/data_reuse_000458","editUrl":"https://github.com/facebook/docusaurus/tree/main/packages/create-docusaurus/templates/shared/blog/2024-09-09-data_reuse_000458.md","source":"@site/blog/2024-09-09-data_reuse_000458.md","title":"Unraveling Anesthesia\'s Effect on Brain Activity","description":"Data Reuse Highlight: This blog entry is part of an ongoing series that showcases examples of data reuse and reanalysis using publicly available data on DANDI.","date":"2024-09-09T00:00:00.000Z","tags":[{"inline":true,"label":"reuse showcase","permalink":"/dandi-docusaurus-example/blog/tags/reuse-showcase"}],"readingTime":8.45,"hasTruncateMarker":false,"authors":[{"name":"Paul Adkisson","key":null,"page":null}],"frontMatter":{"title":"Unraveling Anesthesia\'s Effect on Brain Activity","author":"Paul Adkisson","tags":["reuse showcase"]},"unlisted":false,"nextItem":{"title":"First Blog Post","permalink":"/dandi-docusaurus-example/blog/first-blog-post"}},"content":"_Data Reuse Highlight: This blog entry is part of an ongoing series that showcases examples of data reuse and reanalysis using publicly available data on DANDI._\\n\\nHave you ever wondered what happens to your brain when you\'re under anesthesia? What neural changes coincide with that dramatic loss of consciousness?\\nIn this post, we\'ll explore how two different research teams investigated this fascinating question using the same data from DANDI Archive\'s [Dandiset #000458](https://dandiarchive.org/dandiset/000458).\\n\\n## The Original Study: Peering into the Anesthetized Brain\\nIn June 2023, Dr. Leslie Claar and colleagues from the Allen Institute published their groundbreaking findings in the journal _eLife_ in the article, [\u201cCortico-thalamo-cortical interactions modulate electrically evoked EEG responses in mice\u201d](https://doi.org/10.7554/eLife.84630.3).\\nTheir approach was comprehensive: they stimulated mouse cortex while simultaneously recording with [electroencephelography](https://doi.org/10.1016/B978-141603618-0.10024-4) (EEG) and [neuropixels probes](https://www.neuropixels.org/), comparing brain activity during wakefulness and under isoflurane anesthesia.\\n\\nIn awake mice, Claar\'s team observed a robust [event-related potential](https://doi.org/10.4103/0972-6748.57865) (ERP) when they delivered pulsatile electrical stimulation to deep layers of the [motor cortex](https://doi.org/10.1016/B0-08-043076-7/03460-4) (Figure 1A left).\\nThis ERP coincided with a distinct pattern of neural spiking, both locally in the motor cortex and in distant sensorimotor-related [thalamic nuclei](https://www.ncbi.nlm.nih.gov/books/NBK549908) (Figure 1B left). The response followed a three-act play: initial excitation within 25 milliseconds, a period of quiescence for about 125 milliseconds, and finally, a strong rebound excitation before returning to baseline.\\nWhen the mice were under anesthesia, the story changed dramatically.\\nThe ERP\'s amplitude and complexity plummeted in response to the same stimulation (Figure 1A right).\\nWhile the initial excitation persisted in the motor cortex, it vanished in the thalamic nuclei (Figure 1B right).\\nThe rebound excitation, along with its corresponding ERP component at around 180 milliseconds, was significantly weakened.\\n\\nBased on these observations, Claar and colleagues proposed that interactions between the cortex and thalamus are responsible for the rebound excitation phase of the stimulus response.\\nThis rebound excitation, in turn, drives the second component of the ERP \u2013 a crucial signal of wakefulness.\\n\\nAfter their experiments, Claar and her team published their data on DANDI, ensuring that it would remain publicly accessible for any future researchers who wanted to use it. The dataset and manuscript were prepared and published concurrently, which ensured that the dataset included all the paper-relevant data and metadata and curious reviewers could inspect the data directly if they so wished.\\n\\n![Figure1](/img/blog/data_reuse_000458_elife-fig7.jpg)\\n\\n_**Figure 1: Brain state modulates the ERP via cortico-thalamo-cortical interactions.**\\n(A) Butterfly plot of ERPs during non-running (quiet wakefulness), running (active wakefulness), and isoflurane-anesthetized states.\\n(B) Normalized firing rate, reported as a z-score of the average, pre-stimulus firing rate, of all RS neurons recorded by the Neuropixels probes targeting the stimulated cortex (MO) and SM-TH. Reproduced from \u201cCortico-thalamo-cortical interactions modulate electrically evoked EEG responses in mice\u201d._\\n\\n## A New Perspective: Zooming in on Synaptic Potentials\\nJust a few months later, in November 2023, Drs. Richard Burman, Paul Brodersen and colleagues at the University of Oxford wrote a paper that included reanalysis of this data.\\nTheir findings were published in the journal _Neuron_ with the article, [\u201cActive cortical networks promote shunting fast synaptic inhibition _in vivo_\u201d](https://doi.org/10.1016/j.neuron.2023.08.005).\\nThey also investigated the effects of anesthesia on brain activity, but narrowed their focus to the differential function of a specific receptor subtype: [GABAAR](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2648504/).\\n\\nBurman and Brodersen\'s team used a technique called [_in vivo_ gramicidin perforated patch-clamp recording](https://doi.org/10.1254/fpj.113.339), combined with [optogenetic](https://doi.org/10.1097/ICU.0000000000000140) activation of GABAergic synaptic inputs.\\nThey compared the GABAA receptor equilibrium potential (EGABAAR) in mice during wakefulness and under urethane anesthesia.\\n\\nTheir findings were striking. In anesthetized mice, GABAA receptor responses were significantly hyperpolarizing, with an average equilibrium potential of -80.8mV.\\nIn awake mice, however, these responses could be either hyperpolarizing or depolarizing, averaging at -63.9mV \u2013 a state known as the \\"[shunting](https://en.wikipedia.org/wiki/Shunting_inhibition)\\" regime.\\n\\nBurman et al. hypothesized that the higher network activity in the awake state leads to a higher concentration of intracellular chloride, which in turn depolarizes the GABAA receptor equilibrium potential.\\nAnesthesia, by broadly suppressing neural activity, indirectly hyperpolarizes EGABAAR.\\nThey confirmed this hypothesis by locally injecting an AMPA receptor antagonist ([NBQX](https://www.tocris.com/products/nbqx-disodium-salt_1044)) which suppressed network activity, and hyperpolarized EGABAAR to -78.0mV.\\n\\nThe Oxford researchers also hypothesized that changes in EGABAAR would lead to changes in network activity, resulting in a bi-directional relationship between the two quantities.\\n\\nTo test this hypothesis, Burman and Brodersen\'s team turned to the data from [Dandiset #000458](https://dandiarchive.org/dandiset/000458) \u2013 the very same dataset used by Claar et al.\\nBy reanalyzing this data and manipulating a computational network model, they found that setting the EGABAAR parameter to the shunting regime (-60mV) reproduced spiking responses similar to those seen in awake mice (Figure 2 B&F left).\\nConversely, setting it to the hyperpolarizing regime (-80mV) mimicked the responses seen in anesthetized mice (Figure 2 B&F right).\\nThis data provides evidence for the hypothesized bidirectional relationship.\\n\\n![Figure2](/img/blog/data_reuse_000458_elife-fig7.jpg)\\n\\n_**Figure 2: Shunting inhibition promotes local network desynchronization and response flexibility.**\\n(A) High-density Neuropixels (NPXs) recordings were used to compare spiking activity in the same cortical neurons under awake and anesthetized conditions.\\n(B) Example raster plots show the spiking activity of a population of neurons in somatosensory cortex (SS). Spiking activity is shown across three stimulation trials for each condition.\\n(C) Neuronal synchrony on a trial-to-trial basis (top) and the entropy of the peri-stimulus histogram (bottom) were calculated for each of the 662 recorded neurons under awake and anesthetized conditions (17 probe recordings in 16 mice). Each dot corresponds to a single neuron and the dashed line indicates the line of equality.\\n(D) Neurons in the awake condition exhibited lower synchrony (top; Aw.: 0.325 \xb1 0.005 vs. An.: 0.393 \xb1 0.007, p < 0.001, paired t test) and higher entropy (bottom; Aw.: 1.347 \xb1 0.018 nats vs. An.: 1.111 \xb1 0.020 nats, p < 0.001, paired t test).\\n(E) Schematic of network model consisting of interconnected excitatory pyramidal neurons (Pyr.) and inhibitory interneurons (IN). EGABAAR in the pyramidal neurons was adjusted relative to the RMP to create two different conditions: a shunting (Shunt.) and a hyperpolarizing (Hyperpol.) EGABAAR condition. Spiking activity was evoked by delivering brief depolarizing currents (input) of varying amplitudes to each neuron in the network.\\n(F) Raster plots for the same population of pyramidal neurons (n = 50) in the shunting (left) and hyperpolarizing (right) EGABAAR conditions.\\n(G) Synchrony (top) and entropy (bottom) for pyramidal neurons in the shunting and hyperpolarizing EGABAAR conditions (n = 1,000 randomly selected). Dashed line indicates the line of equality. (H) Neurons in the shunting EGABAAR condition exhibited lower synchrony (Shunt: 0.784 \xb1 0.001 vs. Hyperpol: 0.881 \xb1 0.001, p < 0.001, paired t test) and higher entropy (Shunt: 0.451 \xb1 0.002 nats vs. Hyperpol: 0.197 \xb1 0.002 nats, p < 0.001, paired t test) (n = 16,000 pyramidal neurons from the model). \u2217\u2217\u2217p < 0.001. MO, motor cortex; Stim., electrical stimulation; TH, thalamus. Reproduced from \u201cActive cortical networks promote shunting fast synaptic inhibition in vivo\u201d._\\n\\n## Bridging Scales: From Synapses to Networks\\nTogether, these two studies provide a rich, multi-level account of what happens to brain activity under anesthesia. They span from macroscale brain signals recorded by EEG all the way down to microscale synaptic responses from a single receptor type.\\nThe population spiking data from [Dandiset #000458](https://dandiarchive.org/dandiset/000458) serves as a crucial bridge between these levels, allowing Burman et al. to explicitly connect their work to the broader field of anesthesia research.\\n\\nHowever, it\'s important to note some key differences between the two studies. Claar et al. used isoflurane anesthesia and stimulated layers 5/6 of the cortex, while Burman et al. used urethane anesthesia and recorded from layer 2/3.\\nAdditionally, while Claar et al. found the most dramatic differences between waking and anesthetized states around 180 ms after stimulation, Burman et al. focused their reanalysis on the initial spiking response within 15 ms of stimulation.\\n\\nThese differences highlight the potential for further reanalysis of [Dandiset #000458](https://dandiarchive.org/dandiset/000458).\\nFuture studies could explore more similar experimental conditions, conduct a more thorough treatment of relevant response time periods, or investigate different aspects of anesthesia\'s effects altogether.\\n\\n## Engaging with the Open Science Community\\nFor those inspired by the work of Claar, Burman and Brodersen, and their teams, the journey doesn\u2019t end here.\\nWe encourage readers from all backgrounds to take advantage of this open science platform by publishing and reanalyzing data on DANDI.\\nWe are confident that it will be a smooth and rewarding experience, but don\'t just take our word for it -- listen to what Dr. Leslie Claar and Dr. Paul Brodersen have to say,\\n\\n> \\"After organizing our dataset into NWB format, validating and uploading our files was straightforward using a few simple commands from the DANDI Python Client. \\n> The tools are well-documented, which made the process of uploading and publishing quite smooth. \\n> Within a few months of publishing on DANDI, others were already interested in using our dataset.\\"\\n> -- Dr. Leslie Claar\\n\\n> \u201cThe DANDI archive is a fantastic resource with great tooling and exhaustive documentation. Its command line interface makes data retrieval and synchronization as simple as possible (but not simpler!).\\n> The standardized and well-documented formats for electrophysiological and neuroimaging data ensure that anyone can quickly find their way around a new archive without requiring extensive feedback from the authors that originally collected the data.\\n> These features make the DANDI archive a treasure trove of useful and reusable data, unlike the unfortunately still commonplace unstructured data dumps on many other platforms or -- worst of all -- university servers.\u201d\\n> -- Dr. Paul Brodersen and Professor Colin Akerman\\n\\nTo get you started on your own reanalysis project, we\'ve prepared a Jupyter [notebook](https://github.com/dandi/example-notebooks/blob/master/000458/AllenInstitute/reanalysis.ipynb) demonstrating some basic reanalysis of [Dandiset #000458](https://dandiarchive.org/dandiset/000458).\\nThis tool will help you dive right into the data, guiding you through the initial steps of exploration and analysis.\\nWe have also prepared a Neurosift [walkthrough](https://github.com/dandi/example-notebooks/blob/master/000458/FlatironInstitute/PSTH_neurosift_exploration.md) which demonstrates how to use Neurosift to quickly and easily explore the NWB files in this dataset without even leaving the browser. \\n\\nBy engaging with this open science platform, you become part of a larger community of researchers who are building on each other\'s work and driving the field forward. In the world of open science, collaboration and curiosity drive discovery\u2014so why not see where the data takes you?"},{"id":"first-blog-post","metadata":{"permalink":"/dandi-docusaurus-example/blog/first-blog-post","editUrl":"https://github.com/facebook/docusaurus/tree/main/packages/create-docusaurus/templates/shared/blog/2019-05-28-first-blog-post.mdx","source":"@site/blog/2019-05-28-first-blog-post.mdx","title":"First Blog Post","description":"Lorem ipsum dolor sit amet...","date":"2019-05-28T00:00:00.000Z","tags":[{"inline":false,"label":"Hola","permalink":"/dandi-docusaurus-example/blog/tags/hola","description":"Hola tag description"},{"inline":false,"label":"Docusaurus","permalink":"/dandi-docusaurus-example/blog/tags/docusaurus","description":"Docusaurus tag description"}],"readingTime":0.395,"hasTruncateMarker":true,"authors":[{"name":"Luiz Tauffer","title":"CatalystNeuro","url":"https://github.com/luiztauffer","socials":{"github":"https://github.com/luiztauffer"},"imageURL":"https://avatars.githubusercontent.com/u/24541631?v=4","key":"luiztauffer","page":null},{"name":"Ben Dichter","title":"CatalystNeuro","url":"https://github.com/bendichter","socials":{"github":"https://github.com/bendichter"},"imageURL":"https://avatars.githubusercontent.com/u/844306?v=4","key":"bendichter","page":null}],"frontMatter":{"slug":"first-blog-post","title":"First Blog Post","authors":["luiztauffer","bendichter"],"tags":["hola","docusaurus"]},"unlisted":false,"prevItem":{"title":"Unraveling Anesthesia\'s Effect on Brain Activity","permalink":"/dandi-docusaurus-example/blog/2024/09/09/data_reuse_000458"}},"content":"Lorem ipsum dolor sit amet...\\n\\n{/* truncate */}\\n\\n\\n:::tip\\n\\nExploring data in Neurosift\\n\\n:::\\n\\n...consectetur adipiscing elit. Pellentesque elementum dignissim ultricies. Fusce rhoncus ipsum tempor eros aliquam consequat. Lorem ipsum dolor sit amet\\n\\n\\n\\n\\n\\n\\n\\nWe can add interactive components. For example, use JSX to create an interactive button:\\n\\n```js\\n\\n```\\n\\n"}]}}')}}]); \ No newline at end of file diff --git a/assets/js/runtime~main.5182dd86.js b/assets/js/runtime~main.40bbd299.js similarity index 97% rename from assets/js/runtime~main.5182dd86.js rename to assets/js/runtime~main.40bbd299.js index dad559d..818b52f 100644 --- a/assets/js/runtime~main.5182dd86.js +++ b/assets/js/runtime~main.40bbd299.js @@ -1 +1 @@ -(()=>{"use strict";var e,a,t,r,d,o={},f={};function c(e){var a=f[e];if(void 0!==a)return a.exports;var t=f[e]={id:e,loaded:!1,exports:{}};return o[e].call(t.exports,t,t.exports,c),t.loaded=!0,t.exports}c.m=o,c.c=f,e=[],c.O=(a,t,r,d)=>{if(!t){var o=1/0;for(b=0;b=d)&&Object.keys(c.O).every((e=>c.O[e](t[n])))?t.splice(n--,1):(f=!1,d0&&e[b-1][2]>d;b--)e[b]=e[b-1];e[b]=[t,r,d]},c.n=e=>{var a=e&&e.__esModule?()=>e.default:()=>e;return 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