From 6a885418b2eb9ac7ccf4c58e6112f6e08f5fcee4 Mon Sep 17 00:00:00 2001 From: clbenoit Date: Thu, 8 Feb 2024 14:25:25 +0100 Subject: [PATCH] Continue article about experimental design --- docs/pages/blog/expdesign.mdx | 25 ++++++++++++++++++------- 1 file changed, 18 insertions(+), 7 deletions(-) diff --git a/docs/pages/blog/expdesign.mdx b/docs/pages/blog/expdesign.mdx index 446fa33..79486c9 100644 --- a/docs/pages/blog/expdesign.mdx +++ b/docs/pages/blog/expdesign.mdx @@ -19,7 +19,7 @@ Here's a brief overview of the main considerations for experience designers. ## Next generation sequencing specificities -We need to bear in mind that everything starts with biological material, from which we want to extract informative biological sequences, to answer a precise question. +We need to bear in mind that everything starts with biological material, from which we want to extract **informative** biological sequences, to answer a precise question. The difficulty lies in extracting useful information without confusing it with other factors: background noise, bias... The sample is first taken from its environment, by which it will have been impacted. @@ -28,15 +28,26 @@ as well as the sampling carried out, already constitute a first source of varian # Methodology -1. **Clearly define the question posed and the technology chosen to answer it.** +1. **Clearly define the question posed and the technology chosen to answer it.**
+Many confounding factors will depend on the technology chosen. They will have a greater or lesser impact depending on the question asked. 2. **Control the experimental variables**
Variables to control can include sample preparation techniques, sequencing parameters and environmental conditions. Ensure that the impact of this factors on your data is minimized and controled to isolate the impact of the specific variables of interest, enhancing ythe reliability of the results. -3. **Randomization techniques** -4. **Replication Strategies** - - - +3. **Randomization techniques**
+Applying randomization techniques will prevent your experiment to capture [systemic bias](https://en.wikipedia.org/wiki/Systemic_bias) in your data. +4. **Replication Strategies**
+Replicating the experiment with different samples or under different conditions enhances the robustness of the results. +It allows for the identification of consistent patterns and aids in generalizing findings to a broader context. +5. **Factorial design** +Factorial design is a statistical experimental method that explores the effects of multiple independent variables simultaneously. +It allows researchers to assess the individual effects of each factor and any potential interactions between them, providing a more comprehensive understanding +of experimental outcomes. +# Conclusion + +Experimental design is not so complicated in terms of methodology, and is essential to get the most out of sequencing your biological samples. +However, a thorough knowledge of the different technologies available and of experimental design techniques and strategies is required to apply it effectively... + +# To go further {/* [^1], [^2], [^3] */}