diff --git a/.gitignore b/.gitignore index d7bd2a8..cb11993 100644 --- a/.gitignore +++ b/.gitignore @@ -2,4 +2,5 @@ *.DS_Store *__pycache__/ *.ipynb_checkpoints/ -output/ \ No newline at end of file +output/ + diff --git a/000971/lernerlab/seiler_2024/README.md b/000971/lernerlab/seiler_2024/README.md new file mode 100644 index 0000000..5577e49 --- /dev/null +++ b/000971/lernerlab/seiler_2024/README.md @@ -0,0 +1,8 @@ +# Example Sessions for Dandiset 000971 + +This submission provides 2 notebooks showcasing example sessions for the Dandiset 000971, which corresponds to the 2022 Current Biology paper: [Dopamine signaling in the dorsomedial striatum promotes compulsive behavior](https://doi.org/10.1016/j.cub.2022.01.055.) by Seiler et al. + +Each notebook provides an example of how to access the critical data and metadata from the 2 types of experiments in the dataset: + +- `fiber_photometry_example_notebook.ipynb` showcases one example session from the 000971 dataset containing operant behavior and concurrent fiber photometry recordings. +- `optogenetics_example_notebook.ipynb` showcases one example session from the 000971 dataset containing operant behavior and concurrent excitatory optogenetic stimulation. \ No newline at end of file diff --git a/000971/lernerlab/seiler_2024/__pycache__/stream_nwbfile.cpython-310.pyc b/000971/lernerlab/seiler_2024/__pycache__/stream_nwbfile.cpython-310.pyc new file mode 100644 index 0000000..d2e1f48 Binary files /dev/null and b/000971/lernerlab/seiler_2024/__pycache__/stream_nwbfile.cpython-310.pyc differ diff --git a/000971/lernerlab/seiler_2024/environment.yml b/000971/lernerlab/seiler_2024/environment.yml new file mode 100644 index 0000000..9a11757 --- /dev/null +++ b/000971/lernerlab/seiler_2024/environment.yml @@ -0,0 +1,11 @@ +# run: conda env create --file environment.yaml +name: lerner_notebook_env +channels: + - conda-forge +dependencies: + - python==3.10 + - ipywidgets + - pip + - pip: + - matplotlib + - lerner-lab-to-nwb @ git+https://github.com/catalystneuro/lerner-lab-to-nwb.git@main \ No newline at end of file diff --git a/000971/lernerlab/seiler_2024/fiber_photometry_example_notebook.ipynb b/000971/lernerlab/seiler_2024/fiber_photometry_example_notebook.ipynb new file mode 100644 index 0000000..3cd5da3 --- /dev/null +++ b/000971/lernerlab/seiler_2024/fiber_photometry_example_notebook.ipynb @@ -0,0 +1,524 @@ +{ + "cells": [ + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "# Fiber Photometry Example Session" + ] + }, + { + "cell_type": "code", + "execution_count": 1, + "metadata": {}, + "outputs": [], + "source": [ + "from stream_nwbfile import stream_nwbfile\n", + "import numpy as np\n", + "import matplotlib.pyplot as plt" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "This notebook showcases one example session from the 000971 dataset containing operant behavior and concurrent fiber photometry recordings." + ] + }, + { + "cell_type": "code", + "execution_count": 2, + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "\n", + " \n", + " \n", + " \n", + "
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0 | \n", + "DMS | \n", + "dms_green_fluorophore abc.Indicator at 0x4963995696\\nFields:\\n description: Mice for fiber photometry experiments received infusions of 1ml of AAV5-CAG-FLEX-jGCaMP7b-WPRE (1.02e13 vg/mL, Addgene, lot 18-429) into lateral SNc (AP 3.1, ML 1.3, DV 4.2) in one hemisphere and medial SNc (AP 3.1, ML 0.8, DV 4.7) in the other. Hemispheres were counterbalanced between mice.\\n injection_coordinates_in_mm: [3.1 0.8 4.7]\\n injection_location: medial SNc\\n label: GCaMP7b\\n manufacturer: Addgene\\n | \n", + "optical_fiber abc.OpticalFiber at 0x4963999968\\nFields:\\n core_diameter_in_um: 400.0\\n description: Fiber optic implants (Doric Lenses; 400 um, 0.48 NA) were placed above DMS (AP 0.8, ML 1.5, DV 2.8) and DLS (AP 0.1, ML 2.8, DV 3.5). The DMS implant was placed in the hemisphere receiving a medial SNc viral injection, while the DLS implant was placed in the hemisphere receiving a lateral SNc viral injection. Calcium signals from dopamine terminals in DMS and DLS were recorded during RI30, on the first and last days of RI60/RR20 training as well as on both footshock probes for each mouse. All recordings were done using a fiber photometry rig with optical components from Doric lenses controlled by a real-time processor from Tucker Davis Technologies (TDT; RZ5P). TDT Synapse software was used for data acquisition.\\n manufacturer: Doric Lenses\\n model: Fiber Optic Implant\\n numerical_aperture: 0.48\\n | \n", + "excitation_source_calcium_signal abc.ExcitationSource at 0x4963998384\\nFields:\\n description: 465nm and 405nm LEDs were modulated at 211 Hz and 330 Hz, respectively, for DMS probes. 465nm and 405nm LEDs were modulated at 450 Hz and 270 Hz, respectively for DLS probes. LED currents were adjusted in order to return a voltage between 150-200mV for each signal, were offset by 5 mA, were demodulated using a 4 Hz lowpass frequency filter.\\n excitation_wavelength_in_nm: 465.0\\n illumination_type: LED\\n manufacturer: Doric Lenses\\n model: Connectorized LED\\n | \n", + "photodetector abc.Photodetector at 0x4964001360\\nFields:\\n description: This battery-operated photoreceiver has high gain and detects CW light signals in the sub-picowatt to nanowatt range. When used in conjunction with a modulated light source and a lock-in amplifier to reduce the measurement bandwidth, it achieves sensitivity levels in the femtowatt range. Doric offer this Newport product with add-on fiber optic adapter that improves coupling efficiency between the large core, high NA optical fibers used in Fiber Photometry and relatively small detector area. Its output analog voltage (0-5 V) can be monitored with an oscilloscope or with a DAQ board to record the data with a computer.\\n detected_wavelength_in_nm: 525.0\\n detector_type: photodiode\\n gain: 10000000000.0\\n manufacturer: Doric Lenses\\n model: Newport Visible Femtowatt Photoreceiver Module\\n | \n", + "dichroic_mirror abc.DichroicMirror at 0x4963994496\\nFields:\\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\\n manufacturer: Doric Lenses\\n model: 4 ports Fluorescence Mini Cube - GCaMP\\n | \n", + "[0.8, 1.5, 2.8] | \n", + "commanded_voltage_series_dms abc.CommandedVoltageSeries at 0x4962263024\\nFields:\\n comments: no comments\\n conversion: 1.0\\n data: <HDF5 dataset \"data\": shape (22570496,), type \"<f4\">\\n description: The commanded voltage for the frequency-modulated DMS calcium signal and DMS isosbestic control.\\n frequency__unit: hertz\\n offset: 0.0\\n rate: 6103.515625\\n resolution: -1.0\\n starting_time: 0.0\\n starting_time_unit: seconds\\n unit: volts\\n | \n", + "emission_filter abc.BandOpticalFilter at 0x4963994736\\nFields:\\n bandwidth_in_nm: 50.0\\n center_wavelength_in_nm: 525.0\\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\\n filter_type: Bandpass\\n manufacturer: Doric Lenses\\n model: 4 ports Fluorescence Mini Cube - GCaMP\\n | \n", + "excitation_filter abc.BandOpticalFilter at 0x4963994208\\nFields:\\n bandwidth_in_nm: 30.0\\n center_wavelength_in_nm: 475.0\\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\\n filter_type: Bandpass\\n manufacturer: Doric Lenses\\n model: 4 ports Fluorescence Mini Cube - GCaMP\\n | \n", + "
1 | \n", + "DMS | \n", + "dms_green_fluorophore abc.Indicator at 0x4963995696\\nFields:\\n description: Mice for fiber photometry experiments received infusions of 1ml of AAV5-CAG-FLEX-jGCaMP7b-WPRE (1.02e13 vg/mL, Addgene, lot 18-429) into lateral SNc (AP 3.1, ML 1.3, DV 4.2) in one hemisphere and medial SNc (AP 3.1, ML 0.8, DV 4.7) in the other. Hemispheres were counterbalanced between mice.\\n injection_coordinates_in_mm: [3.1 0.8 4.7]\\n injection_location: medial SNc\\n label: GCaMP7b\\n manufacturer: Addgene\\n | \n", + "optical_fiber abc.OpticalFiber at 0x4963999968\\nFields:\\n core_diameter_in_um: 400.0\\n description: Fiber optic implants (Doric Lenses; 400 um, 0.48 NA) were placed above DMS (AP 0.8, ML 1.5, DV 2.8) and DLS (AP 0.1, ML 2.8, DV 3.5). The DMS implant was placed in the hemisphere receiving a medial SNc viral injection, while the DLS implant was placed in the hemisphere receiving a lateral SNc viral injection. Calcium signals from dopamine terminals in DMS and DLS were recorded during RI30, on the first and last days of RI60/RR20 training as well as on both footshock probes for each mouse. All recordings were done using a fiber photometry rig with optical components from Doric lenses controlled by a real-time processor from Tucker Davis Technologies (TDT; RZ5P). TDT Synapse software was used for data acquisition.\\n manufacturer: Doric Lenses\\n model: Fiber Optic Implant\\n numerical_aperture: 0.48\\n | \n", + "excitation_source_isosbestic_control abc.ExcitationSource at 0x4963998528\\nFields:\\n description: 465nm and 405nm LEDs were modulated at 211 Hz and 330 Hz, respectively, for DMS probes. 465nm and 405nm LEDs were modulated at 450 Hz and 270 Hz, respectively for DLS probes. LED currents were adjusted in order to return a voltage between 150-200mV for each signal, were offset by 5 mA, were demodulated using a 4 Hz lowpass frequency filter.\\n excitation_wavelength_in_nm: 405.0\\n illumination_type: LED\\n manufacturer: Doric Lenses\\n model: Connectorized LED\\n | \n", + "photodetector abc.Photodetector at 0x4964001360\\nFields:\\n description: This battery-operated photoreceiver has high gain and detects CW light signals in the sub-picowatt to nanowatt range. When used in conjunction with a modulated light source and a lock-in amplifier to reduce the measurement bandwidth, it achieves sensitivity levels in the femtowatt range. Doric offer this Newport product with add-on fiber optic adapter that improves coupling efficiency between the large core, high NA optical fibers used in Fiber Photometry and relatively small detector area. Its output analog voltage (0-5 V) can be monitored with an oscilloscope or with a DAQ board to record the data with a computer.\\n detected_wavelength_in_nm: 525.0\\n detector_type: photodiode\\n gain: 10000000000.0\\n manufacturer: Doric Lenses\\n model: Newport Visible Femtowatt Photoreceiver Module\\n | \n", + "dichroic_mirror abc.DichroicMirror at 0x4963994496\\nFields:\\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\\n manufacturer: Doric Lenses\\n model: 4 ports Fluorescence Mini Cube - GCaMP\\n | \n", + "[0.8, 1.5, 2.8] | \n", + "commanded_voltage_series_dms abc.CommandedVoltageSeries at 0x4962263024\\nFields:\\n comments: no comments\\n conversion: 1.0\\n data: <HDF5 dataset \"data\": shape (22570496,), type \"<f4\">\\n description: The commanded voltage for the frequency-modulated DMS calcium signal and DMS isosbestic control.\\n frequency__unit: hertz\\n offset: 0.0\\n rate: 6103.515625\\n resolution: -1.0\\n starting_time: 0.0\\n starting_time_unit: seconds\\n unit: volts\\n | \n", + "emission_filter abc.BandOpticalFilter at 0x4963994736\\nFields:\\n bandwidth_in_nm: 50.0\\n center_wavelength_in_nm: 525.0\\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\\n filter_type: Bandpass\\n manufacturer: Doric Lenses\\n model: 4 ports Fluorescence Mini Cube - GCaMP\\n | \n", + "isosbestic_excitation_filter abc.BandOpticalFilter at 0x4963999008\\nFields:\\n bandwidth_in_nm: 10.0\\n center_wavelength_in_nm: 405.0\\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\\n filter_type: Bandpass\\n manufacturer: Doric Lenses\\n model: 4 ports Fluorescence Mini Cube - GCaMP\\n | \n", + "
2 | \n", + "DLS | \n", + "dls_green_fluorophore abc.Indicator at 0x4962259616\\nFields:\\n description: Mice for fiber photometry experiments received infusions of 1ml of AAV5-CAG-FLEX-jGCaMP7b-WPRE (1.02e13 vg/mL, Addgene, lot 18-429) into lateral SNc (AP 3.1, ML 1.3, DV 4.2) in one hemisphere and medial SNc (AP 3.1, ML 0.8, DV 4.7) in the other. Hemispheres were counterbalanced between mice.\\n injection_coordinates_in_mm: [3.1 1.3 4.2]\\n injection_location: lateral SNc\\n label: GCaMP7b\\n manufacturer: Addgene\\n | \n", + "optical_fiber abc.OpticalFiber at 0x4963999968\\nFields:\\n core_diameter_in_um: 400.0\\n description: Fiber optic implants (Doric Lenses; 400 um, 0.48 NA) were placed above DMS (AP 0.8, ML 1.5, DV 2.8) and DLS (AP 0.1, ML 2.8, DV 3.5). The DMS implant was placed in the hemisphere receiving a medial SNc viral injection, while the DLS implant was placed in the hemisphere receiving a lateral SNc viral injection. Calcium signals from dopamine terminals in DMS and DLS were recorded during RI30, on the first and last days of RI60/RR20 training as well as on both footshock probes for each mouse. All recordings were done using a fiber photometry rig with optical components from Doric lenses controlled by a real-time processor from Tucker Davis Technologies (TDT; RZ5P). TDT Synapse software was used for data acquisition.\\n manufacturer: Doric Lenses\\n model: Fiber Optic Implant\\n numerical_aperture: 0.48\\n | \n", + "excitation_source_calcium_signal abc.ExcitationSource at 0x4963998384\\nFields:\\n description: 465nm and 405nm LEDs were modulated at 211 Hz and 330 Hz, respectively, for DMS probes. 465nm and 405nm LEDs were modulated at 450 Hz and 270 Hz, respectively for DLS probes. LED currents were adjusted in order to return a voltage between 150-200mV for each signal, were offset by 5 mA, were demodulated using a 4 Hz lowpass frequency filter.\\n excitation_wavelength_in_nm: 465.0\\n illumination_type: LED\\n manufacturer: Doric Lenses\\n model: Connectorized LED\\n | \n", + "photodetector abc.Photodetector at 0x4964001360\\nFields:\\n description: This battery-operated photoreceiver has high gain and detects CW light signals in the sub-picowatt to nanowatt range. When used in conjunction with a modulated light source and a lock-in amplifier to reduce the measurement bandwidth, it achieves sensitivity levels in the femtowatt range. Doric offer this Newport product with add-on fiber optic adapter that improves coupling efficiency between the large core, high NA optical fibers used in Fiber Photometry and relatively small detector area. Its output analog voltage (0-5 V) can be monitored with an oscilloscope or with a DAQ board to record the data with a computer.\\n detected_wavelength_in_nm: 525.0\\n detector_type: photodiode\\n gain: 10000000000.0\\n manufacturer: Doric Lenses\\n model: Newport Visible Femtowatt Photoreceiver Module\\n | \n", + "dichroic_mirror abc.DichroicMirror at 0x4963994496\\nFields:\\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\\n manufacturer: Doric Lenses\\n model: 4 ports Fluorescence Mini Cube - GCaMP\\n | \n", + "[0.1, 2.8, 3.5] | \n", + "commanded_voltage_series_dls abc.CommandedVoltageSeries at 0x4962255920\\nFields:\\n comments: no comments\\n conversion: 1.0\\n data: <HDF5 dataset \"data\": shape (22570496,), type \"<f4\">\\n description: The commanded voltage for the frequency-modulated DLS calcium signal and DLS isosbestic control.\\n frequency__unit: hertz\\n offset: 0.0\\n rate: 6103.515625\\n resolution: -1.0\\n starting_time: 0.0\\n starting_time_unit: seconds\\n unit: volts\\n | \n", + "emission_filter abc.BandOpticalFilter at 0x4963994736\\nFields:\\n bandwidth_in_nm: 50.0\\n center_wavelength_in_nm: 525.0\\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\\n filter_type: Bandpass\\n manufacturer: Doric Lenses\\n model: 4 ports Fluorescence Mini Cube - GCaMP\\n | \n", + "excitation_filter abc.BandOpticalFilter at 0x4963994208\\nFields:\\n bandwidth_in_nm: 30.0\\n center_wavelength_in_nm: 475.0\\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\\n filter_type: Bandpass\\n manufacturer: Doric Lenses\\n model: 4 ports Fluorescence Mini Cube - GCaMP\\n | \n", + "
3 | \n", + "DLS | \n", + "dls_green_fluorophore abc.Indicator at 0x4962259616\\nFields:\\n description: Mice for fiber photometry experiments received infusions of 1ml of AAV5-CAG-FLEX-jGCaMP7b-WPRE (1.02e13 vg/mL, Addgene, lot 18-429) into lateral SNc (AP 3.1, ML 1.3, DV 4.2) in one hemisphere and medial SNc (AP 3.1, ML 0.8, DV 4.7) in the other. Hemispheres were counterbalanced between mice.\\n injection_coordinates_in_mm: [3.1 1.3 4.2]\\n injection_location: lateral SNc\\n label: GCaMP7b\\n manufacturer: Addgene\\n | \n", + "optical_fiber abc.OpticalFiber at 0x4963999968\\nFields:\\n core_diameter_in_um: 400.0\\n description: Fiber optic implants (Doric Lenses; 400 um, 0.48 NA) were placed above DMS (AP 0.8, ML 1.5, DV 2.8) and DLS (AP 0.1, ML 2.8, DV 3.5). The DMS implant was placed in the hemisphere receiving a medial SNc viral injection, while the DLS implant was placed in the hemisphere receiving a lateral SNc viral injection. Calcium signals from dopamine terminals in DMS and DLS were recorded during RI30, on the first and last days of RI60/RR20 training as well as on both footshock probes for each mouse. All recordings were done using a fiber photometry rig with optical components from Doric lenses controlled by a real-time processor from Tucker Davis Technologies (TDT; RZ5P). TDT Synapse software was used for data acquisition.\\n manufacturer: Doric Lenses\\n model: Fiber Optic Implant\\n numerical_aperture: 0.48\\n | \n", + "excitation_source_isosbestic_control abc.ExcitationSource at 0x4963998528\\nFields:\\n description: 465nm and 405nm LEDs were modulated at 211 Hz and 330 Hz, respectively, for DMS probes. 465nm and 405nm LEDs were modulated at 450 Hz and 270 Hz, respectively for DLS probes. LED currents were adjusted in order to return a voltage between 150-200mV for each signal, were offset by 5 mA, were demodulated using a 4 Hz lowpass frequency filter.\\n excitation_wavelength_in_nm: 405.0\\n illumination_type: LED\\n manufacturer: Doric Lenses\\n model: Connectorized LED\\n | \n", + "photodetector abc.Photodetector at 0x4964001360\\nFields:\\n description: This battery-operated photoreceiver has high gain and detects CW light signals in the sub-picowatt to nanowatt range. When used in conjunction with a modulated light source and a lock-in amplifier to reduce the measurement bandwidth, it achieves sensitivity levels in the femtowatt range. Doric offer this Newport product with add-on fiber optic adapter that improves coupling efficiency between the large core, high NA optical fibers used in Fiber Photometry and relatively small detector area. Its output analog voltage (0-5 V) can be monitored with an oscilloscope or with a DAQ board to record the data with a computer.\\n detected_wavelength_in_nm: 525.0\\n detector_type: photodiode\\n gain: 10000000000.0\\n manufacturer: Doric Lenses\\n model: Newport Visible Femtowatt Photoreceiver Module\\n | \n", + "dichroic_mirror abc.DichroicMirror at 0x4963994496\\nFields:\\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\\n manufacturer: Doric Lenses\\n model: 4 ports Fluorescence Mini Cube - GCaMP\\n | \n", + "[0.1, 2.8, 3.5] | \n", + "commanded_voltage_series_dls abc.CommandedVoltageSeries at 0x4962255920\\nFields:\\n comments: no comments\\n conversion: 1.0\\n data: <HDF5 dataset \"data\": shape (22570496,), type \"<f4\">\\n description: The commanded voltage for the frequency-modulated DLS calcium signal and DLS isosbestic control.\\n frequency__unit: hertz\\n offset: 0.0\\n rate: 6103.515625\\n resolution: -1.0\\n starting_time: 0.0\\n starting_time_unit: seconds\\n unit: volts\\n | \n", + "emission_filter abc.BandOpticalFilter at 0x4963994736\\nFields:\\n bandwidth_in_nm: 50.0\\n center_wavelength_in_nm: 525.0\\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\\n filter_type: Bandpass\\n manufacturer: Doric Lenses\\n model: 4 ports Fluorescence Mini Cube - GCaMP\\n | \n", + "isosbestic_excitation_filter abc.BandOpticalFilter at 0x4963999008\\nFields:\\n bandwidth_in_nm: 10.0\\n center_wavelength_in_nm: 405.0\\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\\n filter_type: Bandpass\\n manufacturer: Doric Lenses\\n model: 4 ports Fluorescence Mini Cube - GCaMP\\n | \n", + "
\n", + " | location | \n", + "indicator | \n", + "optical_fiber | \n", + "excitation_source | \n", + "photodetector | \n", + "dichroic_mirror | \n", + "coordinates | \n", + "commanded_voltage_series | \n", + "emission_filter | \n", + "excitation_filter | \n", + "
---|---|---|---|---|---|---|---|---|---|---|
id | \n", + "\n", + " | \n", + " | \n", + " | \n", + " | \n", + " | \n", + " | \n", + " | \n", + " | \n", + " | \n", + " |
0 | \n", + "DMS | \n", + "dms_green_fluorophore abc.Indicator at 0x4963995696\\nFields:\\n description: Mice for fiber photometry experiments received infusions of 1ml of AAV5-CAG-FLEX-jGCaMP7b-WPRE (1.02e13 vg/mL, Addgene, lot 18-429) into lateral SNc (AP 3.1, ML 1.3, DV 4.2) in one hemisphere and medial SNc (AP 3.1, ML 0.8, DV 4.7) in the other. Hemispheres were counterbalanced between mice.\\n injection_coordinates_in_mm: [3.1 0.8 4.7]\\n injection_location: medial SNc\\n label: GCaMP7b\\n manufacturer: Addgene\\n | \n", + "optical_fiber abc.OpticalFiber at 0x4963999968\\nFields:\\n core_diameter_in_um: 400.0\\n description: Fiber optic implants (Doric Lenses; 400 um, 0.48 NA) were placed above DMS (AP 0.8, ML 1.5, DV 2.8) and DLS (AP 0.1, ML 2.8, DV 3.5). The DMS implant was placed in the hemisphere receiving a medial SNc viral injection, while the DLS implant was placed in the hemisphere receiving a lateral SNc viral injection. Calcium signals from dopamine terminals in DMS and DLS were recorded during RI30, on the first and last days of RI60/RR20 training as well as on both footshock probes for each mouse. All recordings were done using a fiber photometry rig with optical components from Doric lenses controlled by a real-time processor from Tucker Davis Technologies (TDT; RZ5P). TDT Synapse software was used for data acquisition.\\n manufacturer: Doric Lenses\\n model: Fiber Optic Implant\\n numerical_aperture: 0.48\\n | \n", + "excitation_source_calcium_signal abc.ExcitationSource at 0x4963998384\\nFields:\\n description: 465nm and 405nm LEDs were modulated at 211 Hz and 330 Hz, respectively, for DMS probes. 465nm and 405nm LEDs were modulated at 450 Hz and 270 Hz, respectively for DLS probes. LED currents were adjusted in order to return a voltage between 150-200mV for each signal, were offset by 5 mA, were demodulated using a 4 Hz lowpass frequency filter.\\n excitation_wavelength_in_nm: 465.0\\n illumination_type: LED\\n manufacturer: Doric Lenses\\n model: Connectorized LED\\n | \n", + "photodetector abc.Photodetector at 0x4964001360\\nFields:\\n description: This battery-operated photoreceiver has high gain and detects CW light signals in the sub-picowatt to nanowatt range. When used in conjunction with a modulated light source and a lock-in amplifier to reduce the measurement bandwidth, it achieves sensitivity levels in the femtowatt range. Doric offer this Newport product with add-on fiber optic adapter that improves coupling efficiency between the large core, high NA optical fibers used in Fiber Photometry and relatively small detector area. Its output analog voltage (0-5 V) can be monitored with an oscilloscope or with a DAQ board to record the data with a computer.\\n detected_wavelength_in_nm: 525.0\\n detector_type: photodiode\\n gain: 10000000000.0\\n manufacturer: Doric Lenses\\n model: Newport Visible Femtowatt Photoreceiver Module\\n | \n", + "dichroic_mirror abc.DichroicMirror at 0x4963994496\\nFields:\\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\\n manufacturer: Doric Lenses\\n model: 4 ports Fluorescence Mini Cube - GCaMP\\n | \n", + "[0.8, 1.5, 2.8] | \n", + "commanded_voltage_series_dms abc.CommandedVoltageSeries at 0x4962263024\\nFields:\\n comments: no comments\\n conversion: 1.0\\n data: <HDF5 dataset \"data\": shape (22570496,), type \"<f4\">\\n description: The commanded voltage for the frequency-modulated DMS calcium signal and DMS isosbestic control.\\n frequency__unit: hertz\\n offset: 0.0\\n rate: 6103.515625\\n resolution: -1.0\\n starting_time: 0.0\\n starting_time_unit: seconds\\n unit: volts\\n | \n", + "emission_filter abc.BandOpticalFilter at 0x4963994736\\nFields:\\n bandwidth_in_nm: 50.0\\n center_wavelength_in_nm: 525.0\\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\\n filter_type: Bandpass\\n manufacturer: Doric Lenses\\n model: 4 ports Fluorescence Mini Cube - GCaMP\\n | \n", + "excitation_filter abc.BandOpticalFilter at 0x4963994208\\nFields:\\n bandwidth_in_nm: 30.0\\n center_wavelength_in_nm: 475.0\\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\\n filter_type: Bandpass\\n manufacturer: Doric Lenses\\n model: 4 ports Fluorescence Mini Cube - GCaMP\\n | \n", + "
1 | \n", + "DMS | \n", + "dms_green_fluorophore abc.Indicator at 0x4963995696\\nFields:\\n description: Mice for fiber photometry experiments received infusions of 1ml of AAV5-CAG-FLEX-jGCaMP7b-WPRE (1.02e13 vg/mL, Addgene, lot 18-429) into lateral SNc (AP 3.1, ML 1.3, DV 4.2) in one hemisphere and medial SNc (AP 3.1, ML 0.8, DV 4.7) in the other. Hemispheres were counterbalanced between mice.\\n injection_coordinates_in_mm: [3.1 0.8 4.7]\\n injection_location: medial SNc\\n label: GCaMP7b\\n manufacturer: Addgene\\n | \n", + "optical_fiber abc.OpticalFiber at 0x4963999968\\nFields:\\n core_diameter_in_um: 400.0\\n description: Fiber optic implants (Doric Lenses; 400 um, 0.48 NA) were placed above DMS (AP 0.8, ML 1.5, DV 2.8) and DLS (AP 0.1, ML 2.8, DV 3.5). The DMS implant was placed in the hemisphere receiving a medial SNc viral injection, while the DLS implant was placed in the hemisphere receiving a lateral SNc viral injection. Calcium signals from dopamine terminals in DMS and DLS were recorded during RI30, on the first and last days of RI60/RR20 training as well as on both footshock probes for each mouse. All recordings were done using a fiber photometry rig with optical components from Doric lenses controlled by a real-time processor from Tucker Davis Technologies (TDT; RZ5P). TDT Synapse software was used for data acquisition.\\n manufacturer: Doric Lenses\\n model: Fiber Optic Implant\\n numerical_aperture: 0.48\\n | \n", + "excitation_source_isosbestic_control abc.ExcitationSource at 0x4963998528\\nFields:\\n description: 465nm and 405nm LEDs were modulated at 211 Hz and 330 Hz, respectively, for DMS probes. 465nm and 405nm LEDs were modulated at 450 Hz and 270 Hz, respectively for DLS probes. LED currents were adjusted in order to return a voltage between 150-200mV for each signal, were offset by 5 mA, were demodulated using a 4 Hz lowpass frequency filter.\\n excitation_wavelength_in_nm: 405.0\\n illumination_type: LED\\n manufacturer: Doric Lenses\\n model: Connectorized LED\\n | \n", + "photodetector abc.Photodetector at 0x4964001360\\nFields:\\n description: This battery-operated photoreceiver has high gain and detects CW light signals in the sub-picowatt to nanowatt range. When used in conjunction with a modulated light source and a lock-in amplifier to reduce the measurement bandwidth, it achieves sensitivity levels in the femtowatt range. Doric offer this Newport product with add-on fiber optic adapter that improves coupling efficiency between the large core, high NA optical fibers used in Fiber Photometry and relatively small detector area. Its output analog voltage (0-5 V) can be monitored with an oscilloscope or with a DAQ board to record the data with a computer.\\n detected_wavelength_in_nm: 525.0\\n detector_type: photodiode\\n gain: 10000000000.0\\n manufacturer: Doric Lenses\\n model: Newport Visible Femtowatt Photoreceiver Module\\n | \n", + "dichroic_mirror abc.DichroicMirror at 0x4963994496\\nFields:\\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\\n manufacturer: Doric Lenses\\n model: 4 ports Fluorescence Mini Cube - GCaMP\\n | \n", + "[0.8, 1.5, 2.8] | \n", + "commanded_voltage_series_dms abc.CommandedVoltageSeries at 0x4962263024\\nFields:\\n comments: no comments\\n conversion: 1.0\\n data: <HDF5 dataset \"data\": shape (22570496,), type \"<f4\">\\n description: The commanded voltage for the frequency-modulated DMS calcium signal and DMS isosbestic control.\\n frequency__unit: hertz\\n offset: 0.0\\n rate: 6103.515625\\n resolution: -1.0\\n starting_time: 0.0\\n starting_time_unit: seconds\\n unit: volts\\n | \n", + "emission_filter abc.BandOpticalFilter at 0x4963994736\\nFields:\\n bandwidth_in_nm: 50.0\\n center_wavelength_in_nm: 525.0\\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\\n filter_type: Bandpass\\n manufacturer: Doric Lenses\\n model: 4 ports Fluorescence Mini Cube - GCaMP\\n | \n", + "isosbestic_excitation_filter abc.BandOpticalFilter at 0x4963999008\\nFields:\\n bandwidth_in_nm: 10.0\\n center_wavelength_in_nm: 405.0\\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\\n filter_type: Bandpass\\n manufacturer: Doric Lenses\\n model: 4 ports Fluorescence Mini Cube - GCaMP\\n | \n", + "
2 | \n", + "DLS | \n", + "dls_green_fluorophore abc.Indicator at 0x4962259616\\nFields:\\n description: Mice for fiber photometry experiments received infusions of 1ml of AAV5-CAG-FLEX-jGCaMP7b-WPRE (1.02e13 vg/mL, Addgene, lot 18-429) into lateral SNc (AP 3.1, ML 1.3, DV 4.2) in one hemisphere and medial SNc (AP 3.1, ML 0.8, DV 4.7) in the other. Hemispheres were counterbalanced between mice.\\n injection_coordinates_in_mm: [3.1 1.3 4.2]\\n injection_location: lateral SNc\\n label: GCaMP7b\\n manufacturer: Addgene\\n | \n", + "optical_fiber abc.OpticalFiber at 0x4963999968\\nFields:\\n core_diameter_in_um: 400.0\\n description: Fiber optic implants (Doric Lenses; 400 um, 0.48 NA) were placed above DMS (AP 0.8, ML 1.5, DV 2.8) and DLS (AP 0.1, ML 2.8, DV 3.5). The DMS implant was placed in the hemisphere receiving a medial SNc viral injection, while the DLS implant was placed in the hemisphere receiving a lateral SNc viral injection. Calcium signals from dopamine terminals in DMS and DLS were recorded during RI30, on the first and last days of RI60/RR20 training as well as on both footshock probes for each mouse. All recordings were done using a fiber photometry rig with optical components from Doric lenses controlled by a real-time processor from Tucker Davis Technologies (TDT; RZ5P). TDT Synapse software was used for data acquisition.\\n manufacturer: Doric Lenses\\n model: Fiber Optic Implant\\n numerical_aperture: 0.48\\n | \n", + "excitation_source_calcium_signal abc.ExcitationSource at 0x4963998384\\nFields:\\n description: 465nm and 405nm LEDs were modulated at 211 Hz and 330 Hz, respectively, for DMS probes. 465nm and 405nm LEDs were modulated at 450 Hz and 270 Hz, respectively for DLS probes. LED currents were adjusted in order to return a voltage between 150-200mV for each signal, were offset by 5 mA, were demodulated using a 4 Hz lowpass frequency filter.\\n excitation_wavelength_in_nm: 465.0\\n illumination_type: LED\\n manufacturer: Doric Lenses\\n model: Connectorized LED\\n | \n", + "photodetector abc.Photodetector at 0x4964001360\\nFields:\\n description: This battery-operated photoreceiver has high gain and detects CW light signals in the sub-picowatt to nanowatt range. When used in conjunction with a modulated light source and a lock-in amplifier to reduce the measurement bandwidth, it achieves sensitivity levels in the femtowatt range. Doric offer this Newport product with add-on fiber optic adapter that improves coupling efficiency between the large core, high NA optical fibers used in Fiber Photometry and relatively small detector area. Its output analog voltage (0-5 V) can be monitored with an oscilloscope or with a DAQ board to record the data with a computer.\\n detected_wavelength_in_nm: 525.0\\n detector_type: photodiode\\n gain: 10000000000.0\\n manufacturer: Doric Lenses\\n model: Newport Visible Femtowatt Photoreceiver Module\\n | \n", + "dichroic_mirror abc.DichroicMirror at 0x4963994496\\nFields:\\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\\n manufacturer: Doric Lenses\\n model: 4 ports Fluorescence Mini Cube - GCaMP\\n | \n", + "[0.1, 2.8, 3.5] | \n", + "commanded_voltage_series_dls abc.CommandedVoltageSeries at 0x4962255920\\nFields:\\n comments: no comments\\n conversion: 1.0\\n data: <HDF5 dataset \"data\": shape (22570496,), type \"<f4\">\\n description: The commanded voltage for the frequency-modulated DLS calcium signal and DLS isosbestic control.\\n frequency__unit: hertz\\n offset: 0.0\\n rate: 6103.515625\\n resolution: -1.0\\n starting_time: 0.0\\n starting_time_unit: seconds\\n unit: volts\\n | \n", + "emission_filter abc.BandOpticalFilter at 0x4963994736\\nFields:\\n bandwidth_in_nm: 50.0\\n center_wavelength_in_nm: 525.0\\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\\n filter_type: Bandpass\\n manufacturer: Doric Lenses\\n model: 4 ports Fluorescence Mini Cube - GCaMP\\n | \n", + "excitation_filter abc.BandOpticalFilter at 0x4963994208\\nFields:\\n bandwidth_in_nm: 30.0\\n center_wavelength_in_nm: 475.0\\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\\n filter_type: Bandpass\\n manufacturer: Doric Lenses\\n model: 4 ports Fluorescence Mini Cube - GCaMP\\n | \n", + "
3 | \n", + "DLS | \n", + "dls_green_fluorophore abc.Indicator at 0x4962259616\\nFields:\\n description: Mice for fiber photometry experiments received infusions of 1ml of AAV5-CAG-FLEX-jGCaMP7b-WPRE (1.02e13 vg/mL, Addgene, lot 18-429) into lateral SNc (AP 3.1, ML 1.3, DV 4.2) in one hemisphere and medial SNc (AP 3.1, ML 0.8, DV 4.7) in the other. Hemispheres were counterbalanced between mice.\\n injection_coordinates_in_mm: [3.1 1.3 4.2]\\n injection_location: lateral SNc\\n label: GCaMP7b\\n manufacturer: Addgene\\n | \n", + "optical_fiber abc.OpticalFiber at 0x4963999968\\nFields:\\n core_diameter_in_um: 400.0\\n description: Fiber optic implants (Doric Lenses; 400 um, 0.48 NA) were placed above DMS (AP 0.8, ML 1.5, DV 2.8) and DLS (AP 0.1, ML 2.8, DV 3.5). The DMS implant was placed in the hemisphere receiving a medial SNc viral injection, while the DLS implant was placed in the hemisphere receiving a lateral SNc viral injection. Calcium signals from dopamine terminals in DMS and DLS were recorded during RI30, on the first and last days of RI60/RR20 training as well as on both footshock probes for each mouse. All recordings were done using a fiber photometry rig with optical components from Doric lenses controlled by a real-time processor from Tucker Davis Technologies (TDT; RZ5P). TDT Synapse software was used for data acquisition.\\n manufacturer: Doric Lenses\\n model: Fiber Optic Implant\\n numerical_aperture: 0.48\\n | \n", + "excitation_source_isosbestic_control abc.ExcitationSource at 0x4963998528\\nFields:\\n description: 465nm and 405nm LEDs were modulated at 211 Hz and 330 Hz, respectively, for DMS probes. 465nm and 405nm LEDs were modulated at 450 Hz and 270 Hz, respectively for DLS probes. LED currents were adjusted in order to return a voltage between 150-200mV for each signal, were offset by 5 mA, were demodulated using a 4 Hz lowpass frequency filter.\\n excitation_wavelength_in_nm: 405.0\\n illumination_type: LED\\n manufacturer: Doric Lenses\\n model: Connectorized LED\\n | \n", + "photodetector abc.Photodetector at 0x4964001360\\nFields:\\n description: This battery-operated photoreceiver has high gain and detects CW light signals in the sub-picowatt to nanowatt range. When used in conjunction with a modulated light source and a lock-in amplifier to reduce the measurement bandwidth, it achieves sensitivity levels in the femtowatt range. Doric offer this Newport product with add-on fiber optic adapter that improves coupling efficiency between the large core, high NA optical fibers used in Fiber Photometry and relatively small detector area. Its output analog voltage (0-5 V) can be monitored with an oscilloscope or with a DAQ board to record the data with a computer.\\n detected_wavelength_in_nm: 525.0\\n detector_type: photodiode\\n gain: 10000000000.0\\n manufacturer: Doric Lenses\\n model: Newport Visible Femtowatt Photoreceiver Module\\n | \n", + "dichroic_mirror abc.DichroicMirror at 0x4963994496\\nFields:\\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\\n manufacturer: Doric Lenses\\n model: 4 ports Fluorescence Mini Cube - GCaMP\\n | \n", + "[0.1, 2.8, 3.5] | \n", + "commanded_voltage_series_dls abc.CommandedVoltageSeries at 0x4962255920\\nFields:\\n comments: no comments\\n conversion: 1.0\\n data: <HDF5 dataset \"data\": shape (22570496,), type \"<f4\">\\n description: The commanded voltage for the frequency-modulated DLS calcium signal and DLS isosbestic control.\\n frequency__unit: hertz\\n offset: 0.0\\n rate: 6103.515625\\n resolution: -1.0\\n starting_time: 0.0\\n starting_time_unit: seconds\\n unit: volts\\n | \n", + "emission_filter abc.BandOpticalFilter at 0x4963994736\\nFields:\\n bandwidth_in_nm: 50.0\\n center_wavelength_in_nm: 525.0\\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\\n filter_type: Bandpass\\n manufacturer: Doric Lenses\\n model: 4 ports Fluorescence Mini Cube - GCaMP\\n | \n", + "isosbestic_excitation_filter abc.BandOpticalFilter at 0x4963999008\\nFields:\\n bandwidth_in_nm: 10.0\\n center_wavelength_in_nm: 405.0\\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\\n filter_type: Bandpass\\n manufacturer: Doric Lenses\\n model: 4 ports Fluorescence Mini Cube - GCaMP\\n | \n", + "