diff --git a/doorstop/cli/tests/test_all.py b/doorstop/cli/tests/test_all.py
index e2059da1..79f7979e 100644
--- a/doorstop/cli/tests/test_all.py
+++ b/doorstop/cli/tests/test_all.py
@@ -822,6 +822,15 @@ def test_publish_tree_markdown_with_index(self):
         self.assertTrue(os.path.isdir(path))
         self.assertTrue(os.path.isfile(os.path.join(path, "index.md")))
 
+    def test_publish_tree_markdown_with_index_no_tree(self):
+        """Verify 'doorstop publish' can create Markdown output for a tree,
+        with an index."""
+        path = tempfile.mkdtemp()
+        os.remove(path)
+        self.assertRaises(
+            SystemExit, main(["publish", "all", path, "--markdown", "--index"])
+        )
+
 
 class TestPublishCommand(TempTestCase):
     """Tests 'doorstop publish' options toc and template"""