diff --git a/.kokoro/presubmit/acceptance.cfg b/.kokoro/presubmit/acceptance.cfg
index 93a92eaa0842..232468f796ef 100644
--- a/.kokoro/presubmit/acceptance.cfg
+++ b/.kokoro/presubmit/acceptance.cfg
@@ -29,7 +29,7 @@ env_vars: {
 
 env_vars: {
   key: "SECRET_MANAGER_KEYS"
-  value: ""
+  value: "client-library-test-universe-domain, client-library-test-universe-project-id, client-library-test-universe-storage-location, client-library-test-universe-domain-credential"
 }
 
 env_vars: {
diff --git a/google-cloud-storage/Gemfile b/google-cloud-storage/Gemfile
index aa5496faa85e..a48936104d84 100644
--- a/google-cloud-storage/Gemfile
+++ b/google-cloud-storage/Gemfile
@@ -6,7 +6,6 @@ gem "google-cloud-core", path: "../google-cloud-core"
 gem "google-cloud-errors", path: "../google-cloud-errors"
 gem "google-cloud-pubsub", path: "../google-cloud-pubsub"
 gem "google-cloud-pubsub-v1", path: "../google-cloud-pubsub-v1"
-
 gem "minitest-reporters", "~> 1.5.0", require: false
 gem "rake"
 
diff --git a/google-cloud-storage/acceptance/storage/universe_domain_test.rb b/google-cloud-storage/acceptance/storage/universe_domain_test.rb
new file mode 100644
index 000000000000..e958c6d2a2cb
--- /dev/null
+++ b/google-cloud-storage/acceptance/storage/universe_domain_test.rb
@@ -0,0 +1,68 @@
+# Copyright 2024 Google LLC
+#
+# Licensed under the Apache License, Version 2.0 (the "License");
+# you may not use this file except in compliance with the License.
+# You may obtain a copy of the License at
+#
+#     https://www.apache.org/licenses/LICENSE-2.0
+#
+# Unless required by applicable law or agreed to in writing, software
+# distributed under the License is distributed on an "AS IS" BASIS,
+# WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
+# See the License for the specific language governing permissions and
+# limitations under the License.
+
+require "storage_helper"
+
+describe Google::Cloud::Storage, :universe_domain do
+  
+  # Fetch secret values from the secret_manager path
+  TEST_UNIVERSE_PROJECT_ID = File.read(File.realpath(File.join(ENV["KOKORO_GFILE_DIR"], "secret_manager", "client-library-test-universe-project-id")))
+  TEST_UNIVERSE_LOCATION = File.read(File.realpath(File.join(ENV["KOKORO_GFILE_DIR"], "secret_manager", "client-library-test-universe-storage-location")))
+  TEST_UNIVERSE_DOMAIN = File.read(File.realpath(File.join( ENV["KOKORO_GFILE_DIR"], "secret_manager", "client-library-test-universe-domain")))
+  TEST_UNIVERSE_DOMAIN_CREDENTIAL = File.realpath(File.join( ENV["KOKORO_GFILE_DIR"], "secret_manager", "client-library-test-universe-domain-credential"))
+
+  let :ud_storage do
+    Google::Cloud::Storage.new(
+      project_id: TEST_UNIVERSE_PROJECT_ID,
+      credentials: TEST_UNIVERSE_DOMAIN_CREDENTIAL,
+      universe_domain: TEST_UNIVERSE_DOMAIN
+    )
+  end
+  let(:t) { Time.now.utc.iso8601.gsub ":", "-" }
+  let(:ud_bucket_name) { "ud-test-ruby-bucket-#{t}-#{SecureRandom.hex(4)}".downcase }
+
+  after do
+    ud_bucket = ud_storage.bucket ud_bucket_name
+    if ud_bucket
+      ud_bucket.files.all &:delete
+      safe_gcs_execute { ud_bucket.delete }
+    end
+  end
+
+  it "creates a new bucket and uploads an object with universe_domain" do
+    # Create a bucket
+    ud_bucket =  ud_storage.create_bucket ud_bucket_name, location: TEST_UNIVERSE_LOCATION 
+    _(ud_bucket).wont_be_nil
+    _(ud_bucket.name).must_equal ud_bucket_name
+
+    # Upload an object
+    ud_file_name = "ud-test-file"
+    ud_payload = StringIO.new "Hello world!"
+    ud_file = ud_bucket.create_file ud_payload, ud_file_name
+    _(ud_file.name).must_equal ud_file_name
+
+    # Read the file uploaded
+    uploaded_ud_file = ud_bucket.file ud_file_name
+    _(uploaded_ud_file.name).must_equal ud_file_name
+
+    # Delete the object
+    uploaded_ud_file.delete
+    _(uploaded_ud_file).must_be_nil
+
+    # Delete the bucket
+    ud_bucket.delete
+    _(ud_bucket).must_be_nil
+
+  end
+end