diff --git a/README.md b/README.md index 1d0285e..3787147 100644 --- a/README.md +++ b/README.md @@ -1,5 +1,8 @@ **Admin**: [Getting started](admin/getting_started.md) * [Setting up an analysis](admin/setting_up_an_analysis_guidelines.md) * [Data Management](admin/data_management.md) * [Reproducible Research](admin/reproducible_research.md) * [Methods for manuscripts](admin/method_snippets.md) * [Downloading data](admin/download_data.md) * [Acknowledging funding](admin/acknowledging_funding) * [Initial consults](admin/initial_consults.md) * [Chargeback models](admin/chargeback_models.md) * [Archiving project folders](admin/archive_folders_to_standby.md) * [Consulting resources](admin/consulting_resources.md) * [Pull for knowledge work instead of push](https://dspace.mit.edu/bitstream/handle/1721.1/115364/Repenning_Pull%20for%20Knowledge%20Work_Full.pdf?sequence=1&isAllowed=y) * [Scripts for tracking storage on O2](admin/scripts_for_data_management_on_o2) + +**RC**: * [O2 OpenPortal](rc/O2_portal_errors.md) * [Connection to HPC](rc/connection-to-hpc.md) * [Keep command alive](rc/keepalive.md) * [IPython notebook](rc/ipython-notebook-on-O2.md) * [Manage files](rc/manage-files.md) * [O2 tips](rc/O2-tips.md) * [Scheduler](rc/scheduler.md) * [Jupyter notebooks](rc/jupyter_notebooks.md) * [tmux tips](rc/tmux.md) * [FAS virtual desktops](rc/openondemand.md)*[Arrays in Slurm](rc/arrays_in_slurm.md)*[Common O2 portal Issues](O2_portal_errors.md) + **bcbio**: [docs](https://bcbio-nextgen.readthedocs.io/en/latest/) * [issues](https://github.com/bcbio/bcbio-nextgen/issues) * [validation](https://github.com/bcbio/bcbio_validations) * [running in parallel](https://bcbio-nextgen.readthedocs.io/en/latest/contents/parallel.html) * [genomes](bcbio/bcbio_genomes.md) * [tips](bcbio/bcbio_tips.md) * [RNA-seq workflow by Mary](bcbio/bcbio_workflow_mary.md) * [installation by Michael](https://steinbaugh.com/posts/install-bcbio.html) * [installation by Sergey](https://github.com/naumenko-sa/bioscripts/blob/master/bcbio/bcbio.upgrade.sh) * [RNA-seq workflow by Systems Pharmacology lab](https://labsyspharm.github.io/rnaseq/) * [gtf/gff validators](bcbio/gtf_gff_validator.md) * [Create hybrid mammal virus genome](bcbio/Creating_Hybrid_Mammal_Viral_Reference_Genome.md) * [python package in 2022](https://mathspp.com/blog/how-to-create-a-python-package-in-2022) **Bulk RNA-seq**: [Count normalization methods](https://hbctraining.github.io/DGE_workshop/lessons/02_DGE_count_normalization.html) * [Dexseq](rnaseq/dexseq.Rmd) * [Failure types](rnaseq/failure_types) * [IRFinder_report](rnaseq/IRFinder_report.md) * [RepEnrich2 guide](rnaseq/RepEnrich2_guide.md) * [IRFinder](rnaseq/running_IRFinder.md) * [Strandedness](rnaseq/strandedness.md) * [Tools](rnaseq/tools.md) * [Bibliography](rnaseq/bibliography.md) * [Bibliography.bib](rnaseq/bcbio_rnaseq.bib) * [ASE](rnaseq/ase.md) * [eQTL](http://www.bios.unc.edu/research/genomic_software/Matrix_eQTL/) * [GTEx](https://gtexportal.org/home/) * [SummarizedExperiment templates for QC and DE analyses](https://github.com/naumenko-sa/crt/blob/master/differential_expression/) * [bcbioLite](https://github.com/vbarrera/bcbioLite) * [Cluster profiler](https://yulab-smu.github.io/clusterProfiler-book/) * [ggsashimi](https://github.com/guigolab/ggsashimi) * [Differential exon usage with Suppa2](https://github.com/comprna/SUPPA) * [cell type deconvolution](https://www.synapse.org/#!Synapse:syn15589870/wiki/606797) * [webgestalt](http://www.webgestalt.org/) * [Differential splicing with Leafcutter](https://github.com/hbc/knowledgebase/blob/master/rnaseq/running_leafcutter.md) @@ -16,8 +19,6 @@ **Python**: [Conda on O2](python/conda.md) -**RC**: [Connection to HPC](rc/connection-to-hpc.md) * [Keep command alive](rc/keepalive.md) * [IPython notebook](rc/ipython-notebook-on-O2.md) * [Manage files](rc/manage-files.md) * [O2 tips](rc/O2-tips.md) * [Scheduler](rc/scheduler.md) * [Jupyter notebooks](rc/jupyter_notebooks.md) * [tmux tips](rc/tmux.md) * [FAS virtual desktops](rc/openondemand.md)*[Arrays in Slurm](rc/arrays_in_slurm.md)*[Common O2 portal Issues](O2_portal_errors.md) - **[Single Cell RNA-seq](scrnaseq/README.md):** [Bcbio indrops3](https://bcbio-nextgen.readthedocs.io/en/latest/contents/single_cell.html#workflow) * [Rstudio docker](scrnaseq/rstudio_sc_docker.md) * [Saturation](scrnaseq/saturation_qc.md) * [Clustering analysis in Seurat](scrnaseq/seurat_clustering_analysis.md) * [Seurat markers](scrnaseq/seurat_markers.md) * [Single Cell conda](scrnaseq/Single-Cell-conda.md) * [Cell types](scrnaseq/tinyatlas.md) * [Tools](https://github.com/seandavi/awesome-single-cell) * [Tutorials](scrnaseq/tutorials.md) * [Bibliography](scrnaseq/bibliography.md) * [Velocity](scrnaseq/velocity.md) * [Doublets](scrnaseq/doublets.md) * [Zinbwaver](scrnaseq/zinbwaver.md) * [pseudobulk DESeq2](https://github.com/hbctraining/scRNA-seq_online/blob/master/lessons/pseudobulk_DESeq2_scrnaseq.md) * [pseudobulk edgeR](scrnaseq/pseudobulkDE_edgeR.md) * [CITE-seq](scrnaseq/cite_seq.md) * [Library structures](https://teichlab.github.io/scg_lib_structs/) * [Seurat to 10X counts](scrnaseq/write10Xcounts.md) * [Azimuth](https://azimuth.hubmapconsortium.org/) * [Running Cell Ranger O2](scrnaseq/CellRanger.md) * [Cluster annotation tutorial](https://www.nature.com/articles/s41596-021-00534-0) * [Diff composition analysis](https://github.com/theislab/scCODA) * [OSCA](https://bioconductor.org/books/release/OSCA/) * [upstream TF with pySCENIC](https://github.com/hbc/knowledgebase/blob/master/scrnaseq/pySCENIC.md) * [SC article list for beginners](https://threadreaderapp.com/thread/1577714756047278080.html) * [demuxafy (includes demultiplexing, background and doublet detection tools](https://github.com/hbc/knowledgebase/blob/master/scrnaseq/Demuxafy_HowTo.md) * [Lymphocyte Antigen Receptor Transcripts and Clustering](https://github.com/hbc/knowledgebase/blob/master/scrnaseq/Thoughts_on_lymphocyte_Antigen_Receptor_transcripts_in_sc_RNA_Seq_analyses.md) * [DoubletFinder](https://github.com/hbc/knowledgebase/blob/master/scrnaseq/running_doubletfinder.md) * [Running Mast](https://github.com/hbc/knowledgebase/blob/master/scrnaseq/running_MAST.md) * [Publication Quality UMAPs](https://github.com/hbc/knowledgebase/blob/master/scrnaseq/pub_quality_umaps.md) **Training team**: [github](https://github.com/hbctraining) * [Upcoming workshops](http://bioinformatics.sph.harvard.edu/training) * [Past workshops](http://bioinformatics.sph.harvard.edu/training#past-workshops) * [Workshop materials](https://hbctraining.github.io/main)