About ATAC-seq call peak (hmmratac) and differential analysis via MACS3 #675
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DayTimeMouse
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Hi,
Thanks for developing this function to call peaks for ATAC-Seq.
I ran
macs3 hmmratac -i final.bam -n treat --cutoff-analysis-only
. Then, I got the report(see the attachment), according to the document, -l may set to45
(that can capture moderate number ( about 10k ) of peaks with normal width ( average length 500-1000bps long)), but I'm confused about setting-u
and-c
parameter.The report:
treat_cutoff_analysis.txt
Another question, I have control and treatment, which are no repeats. So, can I use MASC3 to do differential analysis? Could you give me a detail approach?
Thanks in advance.
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