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Western Diet dilution rates differ from dilution rates decided by vmh_template.ipynb #4

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PathogeNish opened this issue Oct 18, 2023 · 0 comments

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@PathogeNish
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PathogeNish commented Oct 18, 2023

The dilution rates in the western_diet.qza are different from the dilution rates decided by the line of code in the vmh_template.ipynb, i.e diet.loc[diet.metabolite.isin(exchanges), "dilution"] = 0.2 .

I wanted to conduct an experiment to see the effect of adding 1gm of fennel (seeds) to the western_diet medium on the observed microbial growth rates. Interestingly, some metabolites (e.g. octadecenoate (n-C18:1)) had higher fluxes in 1gm of fennel (metabolites obtained via vmh diet designer) than in the corresponding flux for the western_diet.

Am I doing something wrong in porting the fluxes from vmh to MICOM? Do I have to do some additional diluting or division by hours on top of the vmh output?

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