diff --git a/modules/nf-core/coptr/estimate/environment.yml b/modules/nf-core/coptr/estimate/environment.yml new file mode 100644 index 00000000000..c5a150067f6 --- /dev/null +++ b/modules/nf-core/coptr/estimate/environment.yml @@ -0,0 +1,7 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +channels: + - conda-forge + - bioconda +dependencies: + - "bioconda::coptr=1.1.4" diff --git a/modules/nf-core/coptr/estimate/main.nf b/modules/nf-core/coptr/estimate/main.nf new file mode 100644 index 00000000000..d16e0a16a4c --- /dev/null +++ b/modules/nf-core/coptr/estimate/main.nf @@ -0,0 +1,47 @@ +process COPTR_ESTIMATE { + tag "$meta.id" + label 'process_low' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/coptr:1.1.4--pyhdfd78af_3': + 'biocontainers/coptr:1.1.4--pyhdfd78af_3' }" + + input: + tuple val(meta), path(pkl, stageAs: "coverage_maps/*") + + output: + tuple val(meta), path("*.csv"), emit: ptr + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + coptr \\ + estimate \\ + $args \\ + coverage_maps/ \\ + ptrs.csv + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + coptr: \$(coptr |& sed -E '11!d ; s/CoPTR.*?\\(v(.*?)\\).*/\\1/') + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.csv + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + coptr: \$(coptr |& sed -E '11!d ; s/CoPTR.*?\\(v(.*?)\\).*/\\1/') + END_VERSIONS + """ +} diff --git a/modules/nf-core/coptr/estimate/meta.yml b/modules/nf-core/coptr/estimate/meta.yml new file mode 100644 index 00000000000..2afb599563a --- /dev/null +++ b/modules/nf-core/coptr/estimate/meta.yml @@ -0,0 +1,56 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "coptr_estimate" +description: Calculates peak-to-through ratio (PTR) from metagenomic sequence data +keywords: + - coptr + - mapping + - ptr +tools: + - "coptr": + description: "Accurate and robust inference of microbial growth dynamics from + metagenomic sequencing reads." + homepage: "https://github.com/tyjo/coptr" + documentation: "https://coptr.readthedocs.io/" + tool_dev_url: "https://github.com/tyjo/coptr" + doi: "10.1101/gr.275533.121" + licence: ["GPL v3"] + identifier: biotools:coptr + +input: + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1', single_end:false ]` + - pkl: + type: file + description: Python pickle file containing coverage maps + pattern: "*.pkl" + ontologies: + - edam: "http://edamontology.org/format_4002" + +output: + - ptr: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1', single_end:false ]` + - "*.csv": + type: file + description: CSV table with rows as reference genomes, columns samples and entries as log2 PTR + pattern: "*.csv" + ontologies: + - edam: "http://edamontology.org/format_3752" + + - versions: + - "versions.yml": + type: file + description: File containing software versions + pattern: "versions.yml" + +authors: + - "@ramirobarrantes" +maintainers: + - "@ramirobarrantes" diff --git a/modules/nf-core/coptr/estimate/tests/main.nf.test b/modules/nf-core/coptr/estimate/tests/main.nf.test new file mode 100644 index 00000000000..949a2108762 --- /dev/null +++ b/modules/nf-core/coptr/estimate/tests/main.nf.test @@ -0,0 +1,71 @@ +nextflow_process { + + name "Test Process COPTR_ESTIMATE" + script "../main.nf" + process "COPTR_ESTIMATE" + + tag "modules" + tag "modules_nfcore" + tag "coptr" + tag "coptr/extract" + tag "coptr/estimate" + + setup { + run("COPTR_EXTRACT") { + script "../../extract/main.nf" + process { + """ + input[0] = [ + [id:'test_0'], + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) + ] + ] + """ + } + } + } + + test("coptr estimate from coverage maps file") { + + when { + process { + """ + input[0] = COPTR_EXTRACT.out.coverage + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() + } + ) + } + + } + + test("coptr estimate from coverage maps file - stub") { + + options "-stub" + + when { + process { + """ + input[0] = COPTR_EXTRACT.out.coverage + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() + } + ) + } + + } + + +} diff --git a/modules/nf-core/coptr/estimate/tests/main.nf.test.snap b/modules/nf-core/coptr/estimate/tests/main.nf.test.snap new file mode 100644 index 00000000000..eb7ff0a32e0 --- /dev/null +++ b/modules/nf-core/coptr/estimate/tests/main.nf.test.snap @@ -0,0 +1,68 @@ +{ + "coptr estimate from coverage maps file - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test_0" + }, + "test_0.csv:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + "versions.yml:md5,5400eb14b9d1287f174065acd6c0c6a4" + ], + "ptr": [ + [ + { + "id": "test_0" + }, + "test_0.csv:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,5400eb14b9d1287f174065acd6c0c6a4" + ] + } + ], + "meta": { + "nf-test": "0.9.2", + "nextflow": "24.10.2" + }, + "timestamp": "2024-12-03T13:46:59.155895" + }, + "coptr estimate from coverage maps file": { + "content": [ + { + "0": [ + [ + { + "id": "test_0" + }, + "ptrs.csv:md5,e94546e830fd9fdcd50e0e6c459f78e5" + ] + ], + "1": [ + "versions.yml:md5,5400eb14b9d1287f174065acd6c0c6a4" + ], + "ptr": [ + [ + { + "id": "test_0" + }, + "ptrs.csv:md5,e94546e830fd9fdcd50e0e6c459f78e5" + ] + ], + "versions": [ + "versions.yml:md5,5400eb14b9d1287f174065acd6c0c6a4" + ] + } + ], + "meta": { + "nf-test": "0.9.2", + "nextflow": "24.10.2" + }, + "timestamp": "2024-11-29T18:28:40.680244" + } +} \ No newline at end of file diff --git a/modules/nf-core/coptr/extract/main.nf b/modules/nf-core/coptr/extract/main.nf index 2633c3907e2..8d9db735bfc 100644 --- a/modules/nf-core/coptr/extract/main.nf +++ b/modules/nf-core/coptr/extract/main.nf @@ -41,8 +41,7 @@ process COPTR_EXTRACT { cat <<-END_VERSIONS > versions.yml "${task.process}": - "${task.process}": - coptr: \$(coptr |& sed -E '11!d ; s/CoPTR.*?\\(v(.*?)\\).*/\\1/') + coptr: \$(coptr |& sed -E '11!d ; s/CoPTR.*?\\(v(.*?)\\).*/\\1/') END_VERSIONS """ } diff --git a/modules/nf-core/coptr/extract/tests/main.nf.test.snap b/modules/nf-core/coptr/extract/tests/main.nf.test.snap index 1fb2eeb35ad..367c20798bf 100644 --- a/modules/nf-core/coptr/extract/tests/main.nf.test.snap +++ b/modules/nf-core/coptr/extract/tests/main.nf.test.snap @@ -83,7 +83,7 @@ ] ], "1": [ - "versions.yml:md5,578f185b99f7f5ba27eb2a9f44c1d6a9" + "versions.yml:md5,c6fcaf8fb6aa9c10a3192d81f9623d4f" ], "coverage": [ [ @@ -94,7 +94,7 @@ ] ], "versions": [ - "versions.yml:md5,578f185b99f7f5ba27eb2a9f44c1d6a9" + "versions.yml:md5,c6fcaf8fb6aa9c10a3192d81f9623d4f" ] } ], @@ -102,6 +102,6 @@ "nf-test": "0.9.2", "nextflow": "24.10.2" }, - "timestamp": "2024-12-02T19:26:21.856688" + "timestamp": "2024-12-03T13:50:47.572605" } } \ No newline at end of file diff --git a/modules/nf-core/coptr/merge/main.nf b/modules/nf-core/coptr/merge/main.nf index 1f3cf2d3a07..c7e6f4df51b 100644 --- a/modules/nf-core/coptr/merge/main.nf +++ b/modules/nf-core/coptr/merge/main.nf @@ -42,7 +42,6 @@ process COPTR_MERGE { cat <<-END_VERSIONS > versions.yml "${task.process}": - "${task.process}": coptr: \$(coptr |& sed -E '11!d ; s/CoPTR.*?\\(v(.*?)\\).*/\\1/') END_VERSIONS """ diff --git a/modules/nf-core/coptr/merge/tests/main.nf.test.snap b/modules/nf-core/coptr/merge/tests/main.nf.test.snap index e52e85b2df3..4fb8da14f1e 100644 --- a/modules/nf-core/coptr/merge/tests/main.nf.test.snap +++ b/modules/nf-core/coptr/merge/tests/main.nf.test.snap @@ -11,7 +11,7 @@ ] ], "1": [ - "versions.yml:md5,65562945dc1e8f43bfa9a6e244bbcdf2" + "versions.yml:md5,86e7803a1ea080ab5b7911c540dd1370" ], "bam": [ [ @@ -22,15 +22,15 @@ ] ], "versions": [ - "versions.yml:md5,65562945dc1e8f43bfa9a6e244bbcdf2" + "versions.yml:md5,86e7803a1ea080ab5b7911c540dd1370" ] } ], "meta": { "nf-test": "0.9.2", - "nextflow": "24.10.1" + "nextflow": "24.10.2" }, - "timestamp": "2024-11-23T14:54:29.748785" + "timestamp": "2024-12-03T13:52:20.557882" }, "coptr merge single bam file": { "content": [