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Error when running illumina command #423

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fractaljc opened this issue Apr 8, 2024 · 0 comments
Open

Error when running illumina command #423

fractaljc opened this issue Apr 8, 2024 · 0 comments
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bug Something isn't working

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@fractaljc
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Description of the bug

This is my first time using nf-core/viralrecon. When trying to run the command with my data I got the error
-- Check script '/home/joancuesta/.nextflow/assets/nf-core/viralrecon/./workflows/illumina.nf' at line: 26 or see '.nextflow.log' file for more details
Could you please help me?

Command used and terminal output

Command used:
nextflow run nf-core/viralrecon --input rv3471.csv --platform illumina --protocol metagenomic --outdir /home/joancuesta/quasispecies/3471 --fasta KX148107.fasta --skip_nextclade -profile docker

Terminal output:
N E X T F L O W  ~  version 23.10.1
Launching `https://github.com/nf-core/viralrecon` [drunk_laplace] DSL2 - revision: 3731dd3a32 [master]


------------------------------------------------------
                                        ,--./,-.
        ___     __   __   __   ___     /,-._.--~'
  |\ | |__  __ /  ` /  \ |__) |__         }  {
  | \| |       \__, \__/ |  \ |___     \`-._,-`-,
                                        `._,._,'
  nf-core/viralrecon v2.6.0-g3731dd3
------------------------------------------------------
Core Nextflow options
  revision       : master
  runName        : drunk_laplace
  containerEngine: docker
  launchDir      : /home/joancuesta/quasispecies
  workDir        : /home/joancuesta/quasispecies/work
  projectDir     : /home/joancuesta/.nextflow/assets/nf-core/viralrecon
  userName       : joancuesta
  profile        : docker
  configFiles    : /home/joancuesta/.nextflow/assets/nf-core/viralrecon/nextflow.config

Input/output options
  input          : rv3471.csv
  platform       : illumina
  protocol       : metagenomic
  outdir         : /home/joancuesta/quasispecies/3471

Reference genome options
  fasta          : KX148107.fasta

Nanopore/Illumina options
  skip_nextclade : true

!! Only displaying parameters that differ from the pipeline defaults !!
------------------------------------------------------
If you use nf-core/viralrecon for your analysis please cite:

* The pipeline
  https://doi.org/10.5281/zenodo.3901628

* The nf-core framework
  https://doi.org/10.1038/s41587-020-0439-x

* Software dependencies
  https://github.com/nf-core/viralrecon/blob/master/CITATIONS.md
------------------------------------------------------
ERROR ~ Access Denied (Service: Amazon S3; Status Code: 403; Error Code: AccessDenied; Request ID: JPCTWMMFEEJEYMKA; S3 Extended Request ID: QYKOf7UzK0VOFoNXCpuPA6erH7Zh2gzklLkmtx3scNhAuIN0sEU4aipQrqACygaLEgk0A2ZTJZE=; Proxy: null)

 -- Check script '/home/joancuesta/.nextflow/assets/nf-core/viralrecon/./workflows/illumina.nf' at line: 26 or see '.nextflow.log' file for more details

Relevant files

.nextflow.log

System information

Nextflow version (23.10.1)
Hardware (x86_64)
Executor (awsbatch)
Container engine: (Docker)
OS (Linux #61~20.04.1-Ubuntu)
Version of nf-core/viralrecon (v2.6.0-g3731dd3)

@fractaljc fractaljc added the bug Something isn't working label Apr 8, 2024
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