diff --git a/applications/nrf5340_audio/src/modules/lc3_file.c b/applications/nrf5340_audio/src/modules/lc3_file.c
index 61bc5018438a..ebbce1d1bd37 100644
--- a/applications/nrf5340_audio/src/modules/lc3_file.c
+++ b/applications/nrf5340_audio/src/modules/lc3_file.c
@@ -12,6 +12,34 @@ LOG_MODULE_REGISTER(sd_card_lc3_file, CONFIG_MODULE_SD_CARD_LC3_FILE_LOG_LEVEL);
 
 #define LC3_FILE_ID 0xCC1C
 
+static void lc3_header_print(struct lc3_file_header *header)
+{
+	if (header == NULL) {
+		LOG_ERR("Nullptr received");
+		return;
+	}
+
+	LOG_DBG("File ID: 0x%04x", header->file_id);
+	LOG_DBG("Header size: %d", header->hdr_size);
+	LOG_DBG("Sample rate: %d Hz", header->sample_rate * 100);
+	LOG_DBG("Bit rate: %d bps", header->bit_rate * 100);
+	LOG_DBG("Channels: %d", header->channels);
+	LOG_DBG("Frame duration: %d us", header->frame_duration * 10);
+	LOG_DBG("Num samples: %d", header->signal_len_lsb | (header->signal_len_msb << 16));
+}
+
+int lc3_header_get(struct lc3_file_ctx const *const file, struct lc3_file_header *header)
+{
+	if ((file == NULL) || (header == NULL)) {
+		LOG_ERR("Nullptr received");
+		return -EINVAL;
+	}
+
+	*header = file->lc3_header;
+
+	return 0;
+}
+
 int lc3_file_frame_get(struct lc3_file_ctx *file, uint8_t *buffer, size_t buffer_size)
 {
 	int ret;
@@ -83,6 +111,9 @@ int lc3_file_open(struct lc3_file_ctx *file, const char *file_name)
 		return ret;
 	}
 
+	/* Debug: Print header */
+	lc3_header_print(&file->lc3_header);
+
 	if (file->lc3_header.file_id != LC3_FILE_ID) {
 		LOG_ERR("Invalid file ID: 0x%04x", file->lc3_header.file_id);
 		return -EINVAL;
diff --git a/applications/nrf5340_audio/src/modules/lc3_file.h b/applications/nrf5340_audio/src/modules/lc3_file.h
index 5cd514179013..d2803bbac6d5 100644
--- a/applications/nrf5340_audio/src/modules/lc3_file.h
+++ b/applications/nrf5340_audio/src/modules/lc3_file.h
@@ -31,6 +31,17 @@ struct lc3_file_ctx {
 	uint32_t number_of_samples;
 };
 
+/**
+ * @brief Get the LC3 header from the file.
+ *
+ * @param[in]	file	Pointer to the file context.
+ * @param[out]	header	Pointer to the header structure to store the header.
+ *
+ * @retval -EINVAL	Invalid file context.
+ * @retval 0		Success.
+ */
+int lc3_header_get(struct lc3_file_ctx const *const file, struct lc3_file_header *header);
+
 /**
  * @brief Get the next LC3 frame from the file.
  *
diff --git a/applications/nrf5340_audio/src/modules/lc3_streamer.c b/applications/nrf5340_audio/src/modules/lc3_streamer.c
index 9384c80ecd11..32784e099d06 100644
--- a/applications/nrf5340_audio/src/modules/lc3_streamer.c
+++ b/applications/nrf5340_audio/src/modules/lc3_streamer.c
@@ -268,6 +268,64 @@ int lc3_streamer_next_frame_get(const uint8_t streamer_idx, const uint8_t **cons
 	return 0;
 }
 
+bool lc3_streamer_file_compatible_check(const char *const filename,
+					const struct lc3_stream_cfg *const cfg)
+{
+	int ret;
+	bool result = true;
+
+	if (filename == NULL || cfg == NULL) {
+		LOG_ERR("NULL pointer received");
+		return false;
+	}
+
+	if (strlen(filename) > CONFIG_FS_FATFS_MAX_LFN - 1) {
+		LOG_ERR("Filename too long");
+		return false;
+	}
+
+	struct lc3_file_ctx file;
+
+	ret = lc3_file_open(&file, filename);
+	if (ret) {
+		LOG_ERR("Failed to open file %d", ret);
+		return false;
+	}
+
+	struct lc3_file_header header;
+
+	ret = lc3_header_get(&file, &header);
+	if (ret) {
+		LOG_WRN("Failed to get header %d", ret);
+		return false;
+	}
+
+	if ((header.sample_rate * 100) != cfg->sample_rate_hz) {
+		LOG_WRN("Sample rate mismatch %d Hz != %d Hz", (header.sample_rate * 100),
+			cfg->sample_rate_hz);
+		result = false;
+	}
+
+	if ((header.bit_rate * 100) != cfg->bit_rate_bps) {
+		LOG_WRN("Bit rate mismatch %d bps != %d bps", (header.bit_rate * 100),
+			cfg->bit_rate_bps);
+		result = false;
+	}
+
+	if ((header.frame_duration * 10) != cfg->frame_duration_us) {
+		LOG_WRN("Frame duration mismatch %d us != %d us", (header.frame_duration * 10),
+			cfg->frame_duration_us);
+		result = false;
+	}
+
+	ret = lc3_file_close(&file);
+	if (ret) {
+		LOG_ERR("Failed to close file %d", ret);
+	}
+
+	return result;
+}
+
 int lc3_streamer_stream_register(const char *const filename, uint8_t *const streamer_idx,
 				 const bool loop)
 {
diff --git a/applications/nrf5340_audio/src/modules/lc3_streamer.h b/applications/nrf5340_audio/src/modules/lc3_streamer.h
index cd43ff8b5de7..3add5e592c65 100644
--- a/applications/nrf5340_audio/src/modules/lc3_streamer.h
+++ b/applications/nrf5340_audio/src/modules/lc3_streamer.h
@@ -12,14 +12,20 @@
 #include <stdbool.h>
 #include <sys/types.h>
 
+struct lc3_stream_cfg {
+	uint32_t sample_rate_hz;
+	uint32_t bit_rate_bps;
+	uint32_t frame_duration_us;
+};
+
 /**
  * @brief Get the next frame for the stream.
  *
- * @details Populates a pointer to the buffer holding the next frame. This buffer is valid until
- *          the next call to this function. or stream is closed.
+ * @details	Populates a pointer to the buffer holding the next frame. This buffer is valid until
+ *		the next call to this function. or stream is closed.
  *
- * @param[in]   streamer_idx    Index of the streamer to get the next frame from.
- * @param[out]  frame_buffer    Pointer to the buffer holding the next frame.
+ * @param[in]	streamer_idx	Index of the streamer to get the next frame from.
+ * @param[out]	frame_buffer	Pointer to the buffer holding the next frame.
  *
  * @retval 0		Success.
  * @retval -EINVAL	Invalid streamer index.
@@ -30,6 +36,22 @@
  */
 int lc3_streamer_next_frame_get(const uint8_t streamer_idx, const uint8_t **const frame_buffer);
 
+/**
+ * @brief Verify that the LC3 header matches the stream configuration.
+ *
+ * @details	Verifies that the file is valid and can be used by the LC3 streamer.
+ *		Since there is no standard header for LC3 files, the header might be different than
+ *		what is defined in the struct lc3_file_header.
+ *
+ * @param[in]	filename	Name of the file to verify.
+ * @param[in]	cfg		Stream configuration to compare against.
+ *
+ * @retval	true	Success.
+ * @retval	false	Header is not matching the configuration.
+ */
+bool lc3_streamer_file_compatible_check(const char *const filename,
+					const struct lc3_stream_cfg *const cfg);
+
 /**
  * @brief Register a new stream that will be played by the LC3 streamer.
  *
@@ -63,10 +85,10 @@ uint8_t lc3_streamer_num_active_streams(void);
  *          truncated.
  *
  * @param[in]	streamer_idx	Index of the streamer.
- * @param[out]	path		Pointer for string to store filepath in.
+ * @param[out]	path		Pointer for string to store file path in.
  * @param[in]	path_len	Length of string buffer.
  *
- * @retval	-EINVAL		Nullpointers or invalid index given.
+ * @retval	-EINVAL		Null pointers or invalid index given.
  * @retval	0		Success.
  */
 int lc3_streamer_file_path_get(const uint8_t streamer_idx, char *const path, const size_t path_len);
diff --git a/samples/bluetooth/broadcast_config_tool/Kconfig b/samples/bluetooth/broadcast_config_tool/Kconfig
index c01b6d9a80f8..4f2efb91f6ca 100644
--- a/samples/bluetooth/broadcast_config_tool/Kconfig
+++ b/samples/bluetooth/broadcast_config_tool/Kconfig
@@ -14,6 +14,12 @@ rsource "${ZEPHYR_NRF_MODULE_DIR}/applications/nrf5340_audio/src/utils/Kconfig"
 config TRANSPORT_BIS
 	bool "Use BIS (Broadcast Isochronous Stream)"
 
+config BROADCAST_CONFIG_TOOL
+	bool "Broadcast Configuration Tool"
+	depends on TRANSPORT_BIS
+	select EXPERIMENTAL
+	default y
+
 menu "Logging"
 
 module = MAIN
diff --git a/samples/bluetooth/broadcast_config_tool/src/main.c b/samples/bluetooth/broadcast_config_tool/src/main.c
index a6232b4eb025..03f5c57f74e3 100644
--- a/samples/bluetooth/broadcast_config_tool/src/main.c
+++ b/samples/bluetooth/broadcast_config_tool/src/main.c
@@ -1584,6 +1584,36 @@ static int cmd_file_select(const struct shell *shell, size_t argc, char **argv)
 	LOG_DBG("Selecting file %s for stream big: %d sub: %d bis: %d", file_name, big_index,
 		sub_index, bis_index);
 
+	struct bt_audio_codec_cfg *codec_cfg =
+		&broadcast_param[big_index].subgroups[sub_index].group_lc3_preset.codec_cfg;
+
+	struct lc3_stream_cfg cfg;
+
+	ret = le_audio_freq_hz_get(codec_cfg, &cfg.sample_rate_hz);
+	if (ret) {
+		shell_error(shell, "Failed to get frequency: %d", ret);
+		return ret;
+	}
+
+	ret = le_audio_duration_us_get(codec_cfg, &cfg.frame_duration_us);
+	if (ret) {
+		shell_error(shell, "Failed to get frame duration: %d", ret);
+	}
+
+	ret = le_audio_bitrate_get(codec_cfg, &cfg.bit_rate_bps);
+	if (ret) {
+		shell_error(shell, "Failed to get bitrate: %d", ret);
+	}
+
+	/* Verify that the file header matches the stream configurationn */
+	/* NOTE: This will not abort the streamer if the file is not valid, only give a warning */
+	bool header_valid = lc3_streamer_file_compatible_check(file_name, &cfg);
+
+	if (!header_valid) {
+		shell_warn(shell, "File header verification failed. File may not be compatible "
+				  "with stream config.");
+	}
+
 	ret = lc3_streamer_stream_register(
 		file_name, &lc3_stream_infos[big_index][sub_index].lc3_streamer_idx[bis_index],
 		true);