diff --git a/instrumentation/sequel/.rubocop.yml b/instrumentation/sequel/.rubocop.yml
new file mode 100644
index 0000000000..1248a2f825
--- /dev/null
+++ b/instrumentation/sequel/.rubocop.yml
@@ -0,0 +1 @@
+inherit_from: ../../.rubocop.yml
diff --git a/instrumentation/sequel/.yardopts b/instrumentation/sequel/.yardopts
new file mode 100644
index 0000000000..052ed47a6c
--- /dev/null
+++ b/instrumentation/sequel/.yardopts
@@ -0,0 +1,9 @@
+--no-private
+--title=OpenTelemetry Sequel Instrumentation
+--markup=markdown
+--main=README.md
+./lib/opentelemetry/instrumentation/**/*.rb
+./lib/opentelemetry/instrumentation.rb
+-
+README.md
+CHANGELOG.md
diff --git a/instrumentation/sequel/CHANGELOG.md b/instrumentation/sequel/CHANGELOG.md
new file mode 100644
index 0000000000..3683f330f3
--- /dev/null
+++ b/instrumentation/sequel/CHANGELOG.md
@@ -0,0 +1,5 @@
+# Release History: opentelemetry-instrumentation-sequel
+
+### v0.1.0 / 2023-09-30
+
+* Initial release
diff --git a/instrumentation/sequel/Gemfile b/instrumentation/sequel/Gemfile
new file mode 100644
index 0000000000..2baf57ac47
--- /dev/null
+++ b/instrumentation/sequel/Gemfile
@@ -0,0 +1,13 @@
+# frozen_string_literal: true
+
+# Copyright The OpenTelemetry Authors
+#
+# SPDX-License-Identifier: Apache-2.0
+
+source 'https://rubygems.org'
+
+gemspec
+
+group :test do
+  gem 'opentelemetry-instrumentation-base', path: '../base'
+end
diff --git a/instrumentation/sequel/README.md b/instrumentation/sequel/README.md
new file mode 100644
index 0000000000..aa397fd978
--- /dev/null
+++ b/instrumentation/sequel/README.md
@@ -0,0 +1,67 @@
+# Sequel Instrumentation
+
+The Sequel instrumentation is a community-maintained instrumentation for the [Sequel][sequel-home] Database Toolkit.
+
+## How do I get started?
+
+Install the gem using:
+
+```
+gem install opentelemetry-instrumentation-sequel
+```
+
+Or, if you use [bundler][bundler-home], include `opentelemetry-instrumentation-sequel` to your `Gemfile`.
+
+## Usage
+
+To install the instrumentation, add the gem to your Gemfile:
+
+```ruby
+gem 'opentelemetry-instrumentation-sequel'
+```
+
+Then call `use` with the name of the instrumentation:
+
+```ruby
+require 'rubygems'
+require 'bundler/setup'
+
+Bundler.require
+
+OpenTelemetry::SDK.configure do |c|
+  c.use 'OpenTelemetry::Instrumentation::Sequel'
+end
+```
+
+Alternatively, you can also call `use_all` to install all the available instrumentation.
+
+```ruby
+OpenTelemetry::SDK.configure do |c|
+  c.use_all
+end
+```
+
+Lastly enable the extension on your DB connection:
+
+```ruby
+DB = Sequel.connect('sqlite::memory:')
+DB.extension :opentelemetry
+```
+
+## How can I get involved?
+
+The `opentelemetry-instrumentation-sequel` gem source is [on github][repo-github], along with related gems including `opentelemetry-api` and `opentelemetry-sdk`.
+
+The OpenTelemetry Ruby gems are maintained by the OpenTelemetry-Ruby special interest group (SIG). You can get involved by joining us in [GitHub Discussions][discussions-url] or attending our weekly meeting. See the [meeting calendar][community-meetings] for dates and times. For more information on this and other language SIGs, see the OpenTelemetry [community page][ruby-sig].
+
+## License
+
+The `opentelemetry-instrumentation-sequel` gem is distributed under the Apache 2.0 license. See [LICENSE][license-github] for more information.
+
+[sequel-home]: https://sequel.jeremyevans.net/
+[bundler-home]: https://bundler.io
+[repo-github]: https://github.com/open-telemetry/opentelemetry-ruby
+[license-github]: https://github.com/open-telemetry/opentelemetry-ruby-contrib/blob/main/LICENSE
+[ruby-sig]: https://github.com/open-telemetry/community#ruby-sig
+[community-meetings]: https://github.com/open-telemetry/community#community-meetings
+[discussions-url]: https://github.com/open-telemetry/opentelemetry-ruby/discussions
diff --git a/instrumentation/sequel/Rakefile b/instrumentation/sequel/Rakefile
new file mode 100644
index 0000000000..1a64ba842e
--- /dev/null
+++ b/instrumentation/sequel/Rakefile
@@ -0,0 +1,28 @@
+# frozen_string_literal: true
+
+# Copyright The OpenTelemetry Authors
+#
+# SPDX-License-Identifier: Apache-2.0
+
+require 'bundler/gem_tasks'
+require 'rake/testtask'
+require 'yard'
+require 'rubocop/rake_task'
+
+RuboCop::RakeTask.new
+
+Rake::TestTask.new :test do |t|
+  t.libs << 'test'
+  t.libs << 'lib'
+  t.test_files = FileList['test/**/*_test.rb']
+end
+
+YARD::Rake::YardocTask.new do |t|
+  t.stats_options = ['--list-undoc']
+end
+
+if RUBY_ENGINE == 'truffleruby'
+  task default: %i[test]
+else
+  task default: %i[test rubocop yard]
+end
diff --git a/instrumentation/sequel/lib/opentelemetry-instrumentation-sequel.rb b/instrumentation/sequel/lib/opentelemetry-instrumentation-sequel.rb
new file mode 100644
index 0000000000..5ebcc77b3e
--- /dev/null
+++ b/instrumentation/sequel/lib/opentelemetry-instrumentation-sequel.rb
@@ -0,0 +1,7 @@
+# frozen_string_literal: true
+
+# Copyright The OpenTelemetry Authors
+#
+# SPDX-License-Identifier: Apache-2.0
+
+require_relative 'opentelemetry/sequel'
diff --git a/instrumentation/sequel/lib/opentelemetry/instrumentation/sequel.rb b/instrumentation/sequel/lib/opentelemetry/instrumentation/sequel.rb
new file mode 100644
index 0000000000..8444b9c829
--- /dev/null
+++ b/instrumentation/sequel/lib/opentelemetry/instrumentation/sequel.rb
@@ -0,0 +1,19 @@
+# frozen_string_literal: true
+
+# Copyright The OpenTelemetry Authors
+#
+# SPDX-License-Identifier: Apache-2.0
+
+require 'opentelemetry'
+require 'opentelemetry-instrumentation-base'
+
+module OpenTelemetry
+  module Instrumentation
+    # Contains the OpenTelemetry instrumentation for the Sequel gem
+    module Sequel
+    end
+  end
+end
+
+require_relative 'sequel/instrumentation'
+require_relative 'sequel/version'
diff --git a/instrumentation/sequel/lib/opentelemetry/instrumentation/sequel/instrumentation.rb b/instrumentation/sequel/lib/opentelemetry/instrumentation/sequel/instrumentation.rb
new file mode 100644
index 0000000000..4299b6e4e5
--- /dev/null
+++ b/instrumentation/sequel/lib/opentelemetry/instrumentation/sequel/instrumentation.rb
@@ -0,0 +1,25 @@
+# frozen_string_literal: true
+
+# Copyright The OpenTelemetry Authors
+#
+# SPDX-License-Identifier: Apache-2.0
+
+require 'sequel/extensions/opentelemetry'
+
+module OpenTelemetry
+  module Instrumentation
+    module Sequel
+      # The Instrumentation class contains logic to detect and install the Sequel
+      # instrumentation
+      class Instrumentation < OpenTelemetry::Instrumentation::Base
+        install do |_|
+          ::Sequel.extension(:opentelemetry)
+        end
+
+        present do
+          defined?(::Sequel)
+        end
+      end
+    end
+  end
+end
diff --git a/instrumentation/sequel/lib/opentelemetry/instrumentation/sequel/version.rb b/instrumentation/sequel/lib/opentelemetry/instrumentation/sequel/version.rb
new file mode 100644
index 0000000000..add23fa4bc
--- /dev/null
+++ b/instrumentation/sequel/lib/opentelemetry/instrumentation/sequel/version.rb
@@ -0,0 +1,13 @@
+# frozen_string_literal: true
+
+# Copyright The OpenTelemetry Authors
+#
+# SPDX-License-Identifier: Apache-2.0
+
+module OpenTelemetry
+  module Instrumentation
+    module Sequel
+      VERSION = '0.1.0'
+    end
+  end
+end
diff --git a/instrumentation/sequel/lib/opentelemetry/sequel.rb b/instrumentation/sequel/lib/opentelemetry/sequel.rb
new file mode 100644
index 0000000000..cea4a83a42
--- /dev/null
+++ b/instrumentation/sequel/lib/opentelemetry/sequel.rb
@@ -0,0 +1,22 @@
+# frozen_string_literal: true
+
+# Copyright The OpenTelemetry Authors
+#
+# SPDX-License-Identifier: Apache-2.0
+
+# OpenTelemetry is an open source observability framework, providing a
+# general-purpose API, SDK, and related tools required for the instrumentation
+# of cloud-native software, frameworks, and libraries.
+#
+# The OpenTelemetry module provides global accessors for telemetry objects.
+# See the documentation for the `opentelemetry-api` gem for details.
+module OpenTelemetry
+  # "Instrumentation" are specified by
+  # https://github.com/open-telemetry/opentelemetry-specification/blob/784635d01d8690c8f5fcd1f55bdbc8a13cf2f4f2/specification/glossary.md#instrumentation-library
+  #
+  # Instrumentation should be able to handle the case when the library is not installed on a user's system.
+  module Instrumentation
+  end
+end
+
+require_relative 'instrumentation/sequel'
diff --git a/instrumentation/sequel/lib/sequel/extensions/opentelemetry.rb b/instrumentation/sequel/lib/sequel/extensions/opentelemetry.rb
new file mode 100644
index 0000000000..9538ded8cb
--- /dev/null
+++ b/instrumentation/sequel/lib/sequel/extensions/opentelemetry.rb
@@ -0,0 +1,46 @@
+# frozen_string_literal: true
+
+require 'sequel'
+
+# Sequel: The Database Toolkit for Ruby
+module Sequel
+  # The Sequel::Opentelemetry extension
+  #
+  # @example Simple usage
+  #
+  #     require 'sequel-opentelemetry'
+  #     DB.extension :opentelemetry
+  #
+  # Related module: Sequel::Opentelemetry
+  module Opentelemetry
+    def tracer
+      @tracer ||= ::OpenTelemetry.tracer_provider.tracer('Sequel', ::Sequel::VERSION)
+    end
+
+    def trace_execute(super_method, sequel_method, sql, options, &block)
+      response = nil
+      attributes = {
+        'component' => 'Sequel',
+        ::OpenTelemetry::SemanticConventions::Trace::DB_NAME => opts[:database],
+        ::OpenTelemetry::SemanticConventions::Trace::DB_SYSTEM => database_type.to_s,
+        ::OpenTelemetry::SemanticConventions::Trace::DB_STATEMENT => sql,
+        ::OpenTelemetry::SemanticConventions::Trace::DB_OPERATION => sequel_method
+      }
+      attributes['sequel.host'] = opts[:host] if opts[:host]
+
+      tracer.in_span("sequel.#{sequel_method}", attributes: attributes) do
+        response = super_method.call(sql, options, &block)
+      end
+      response
+    end
+
+    def execute(sql, options = ::Sequel::OPTS, &block)
+      trace_execute(proc { super(sql, options, &block) }, 'execute', sql, options, &block)
+    end
+
+    def execute_dui(sql, options = ::Sequel::OPTS, &block)
+      trace_execute(proc { super(sql, options, &block) }, 'execute_dui', sql, options, &block)
+    end
+  end
+  Database.register_extension(:opentelemetry, Opentelemetry)
+end
diff --git a/instrumentation/sequel/opentelemetry-instrumentation-sequel.gemspec b/instrumentation/sequel/opentelemetry-instrumentation-sequel.gemspec
new file mode 100644
index 0000000000..907c8adce1
--- /dev/null
+++ b/instrumentation/sequel/opentelemetry-instrumentation-sequel.gemspec
@@ -0,0 +1,49 @@
+# frozen_string_literal: true
+
+# Copyright The OpenTelemetry Authors
+#
+# SPDX-License-Identifier: Apache-2.0
+
+lib = File.expand_path('lib', __dir__)
+$LOAD_PATH.unshift(lib) unless $LOAD_PATH.include?(lib)
+require 'opentelemetry/instrumentation/sequel/version'
+
+Gem::Specification.new do |spec|
+  spec.name        = 'opentelemetry-instrumentation-sequel'
+  spec.version     = OpenTelemetry::Instrumentation::Sequel::VERSION
+  spec.authors     = ['OpenTelemetry Authors']
+  spec.email       = ['cncf-opentelemetry-contributors@lists.cncf.io']
+
+  spec.summary     = 'Sequel instrumentation for the OpenTelemetry framework'
+  spec.description = 'Sequel instrumentation for the OpenTelemetry framework'
+  spec.homepage    = 'https://github.com/open-telemetry/opentelemetry-ruby-contrib'
+  spec.license     = 'Apache-2.0'
+
+  spec.files = Dir.glob('lib/**/*.rb') +
+               Dir.glob('*.md') +
+               ['LICENSE', '.yardopts']
+  spec.require_paths = ['lib']
+  spec.required_ruby_version = '>= 3.0'
+
+  spec.add_dependency 'opentelemetry-api', '~> 1.0'
+  spec.add_dependency 'opentelemetry-common', '~> 0.20.0'
+  spec.add_dependency 'opentelemetry-instrumentation-base', '~> 0.22.1'
+
+  spec.add_development_dependency 'appraisal', '~> 2.5'
+  spec.add_development_dependency 'bundler', '~> 2.4'
+  spec.add_development_dependency 'minitest', '~> 5.0'
+  spec.add_development_dependency 'opentelemetry-sdk', '~> 1.1'
+  spec.add_development_dependency 'opentelemetry-test-helpers', '~> 0.3'
+  spec.add_development_dependency 'rubocop', '~> 1.56.1'
+  spec.add_development_dependency 'sequel', '~> 5.72.0'
+  spec.add_development_dependency 'simplecov', '~> 0.17.1'
+  spec.add_development_dependency 'sqlite3', '~> 1.6.4'
+  spec.add_development_dependency 'yard', '~> 0.9'
+
+  if spec.respond_to?(:metadata)
+    spec.metadata['changelog_uri'] = "https://rubydoc.info/gems/#{spec.name}/#{spec.version}/file/CHANGELOG.md"
+    spec.metadata['source_code_uri'] = 'https://github.com/open-telemetry/opentelemetry-ruby-contrib/tree/main/instrumentation/sequel'
+    spec.metadata['bug_tracker_uri'] = 'https://github.com/open-telemetry/opentelemetry-ruby-contrib/issues'
+    spec.metadata['documentation_uri'] = "https://rubydoc.info/gems/#{spec.name}/#{spec.version}"
+  end
+end
diff --git a/instrumentation/sequel/test/opentelemetry/instrumentation/sequel_test.rb b/instrumentation/sequel/test/opentelemetry/instrumentation/sequel_test.rb
new file mode 100644
index 0000000000..30b4540140
--- /dev/null
+++ b/instrumentation/sequel/test/opentelemetry/instrumentation/sequel_test.rb
@@ -0,0 +1,76 @@
+# frozen_string_literal: true
+
+# Copyright The OpenTelemetry Authors
+#
+# SPDX-License-Identifier: Apache-2.0
+
+require 'test_helper'
+
+describe OpenTelemetry::Instrumentation::Sequel do
+  let(:instrumentation) { OpenTelemetry::Instrumentation::Sequel::Instrumentation.instance }
+  let(:exporter) { EXPORTER }
+  let(:database) { Sequel.connect('sqlite::memory:') }
+  let(:model) { Class.new(Sequel::Model(:documents)) }
+
+  # class CustomError < StandardError; end
+
+  before do
+    instrumentation.install
+    database.extension :opentelemetry
+    database.create_table!(:documents) do
+      primary_key :id
+      String :title
+    end
+    model # Instantiate the model so that it doesn't interfere with further calls
+
+    exporter.reset
+  end
+
+  describe 'tracing' do
+    it 'before query' do
+      _(exporter.finished_spans.size).must_equal 0
+    end
+
+    it 'traces dataset executes' do
+      database['SELECT 1'].all
+
+      _(exporter.finished_spans.size).must_equal 1
+    end
+
+    it 'traces direct executes' do
+      database.run('SELECT 1')
+
+      _(exporter.finished_spans.size).must_equal 1
+    end
+
+    it 'traces models' do
+      model.first
+
+      _(exporter.finished_spans.size).must_equal 1
+    end
+
+    it 'records attributes' do
+      model.first
+
+      _(exporter.finished_spans.first.attributes).must_equal(
+        'component' => 'Sequel',
+        'db.name' => '',
+        'db.system' => 'sqlite',
+        'db.statement' => 'SELECT * FROM `documents` LIMIT 1',
+        'db.operation' => 'execute'
+      )
+    end
+
+    it 'traces DUI calls' do
+      database.create_table(:test_table) { primary_key :id }
+
+      _(exporter.finished_spans.first.attributes).must_equal(
+        'component' => 'Sequel',
+        'db.name' => '',
+        'db.system' => 'sqlite',
+        'db.statement' => 'CREATE TABLE `test_table` (`id` integer NOT NULL PRIMARY KEY AUTOINCREMENT)',
+        'db.operation' => 'execute_dui'
+      )
+    end
+  end
+end
diff --git a/instrumentation/sequel/test/test_helper.rb b/instrumentation/sequel/test/test_helper.rb
new file mode 100644
index 0000000000..d75b1d3403
--- /dev/null
+++ b/instrumentation/sequel/test/test_helper.rb
@@ -0,0 +1,22 @@
+# frozen_string_literal: true
+
+# Copyright The OpenTelemetry Authors
+#
+# SPDX-License-Identifier: Apache-2.0
+
+require 'bundler/setup'
+Bundler.require(:default, :development, :test)
+
+require 'minitest/autorun'
+
+require 'opentelemetry-instrumentation-sequel'
+
+# global opentelemetry-sdk setup:
+EXPORTER = OpenTelemetry::SDK::Trace::Export::InMemorySpanExporter.new
+span_processor = OpenTelemetry::SDK::Trace::Export::SimpleSpanProcessor.new(EXPORTER)
+
+OpenTelemetry::SDK.configure do |c|
+  c.error_handler = ->(exception:, message:) { raise(exception || message) }
+  c.logger = Logger.new($stderr, level: ENV.fetch('OTEL_LOG_LEVEL', 'fatal').to_sym)
+  c.add_span_processor span_processor
+end