diff --git a/openff/bespokefit/tests/conftest.py b/openff/bespokefit/tests/conftest.py
index 2cba3519..6cf62fbe 100644
--- a/openff/bespokefit/tests/conftest.py
+++ b/openff/bespokefit/tests/conftest.py
@@ -87,7 +87,6 @@ def clear_force_balance_caches():
         OE_TOOLKIT_CACHE_molecule_conformers,
         RDK_TOOLKIT_CACHE_find_smarts_matches,
         RDK_TOOLKIT_CACHE_molecule_conformers,
-        TOOLKIT_CACHE_ChemicalEnvironment_validate,
     )
 
     OE_TOOLKIT_CACHE_find_smarts_matches.clear()