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Setup Extensions and Modifications |
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Additional setup tweaks |
Michael Redd from the Cell Imaging Core Facility at the University of Utah put together an Arduino-based solution to get proper bright-field illumination in an OpenSPIM setup: Brightfield.
Instructions to make an Arduino-controlled shutter compatible with uManager: paper by Todd P. Meyrath and tutorial on the uManager page.
If you would like to swap out one of the Picard stepper motors you can do so with a bit of effort.
The original OpenSPIM uses a 2X expander to enlarge the laser beam to 2 mm. For larger samples a larger field of view can be preferable. This means that you will need a larger beam to create a taller light sheet. Change the second beam expander lens to f=75 mm or 100 mm and increase the distance between lenses accordingly.
Immersion objectives with long working distance are hard to find. However, at low NA and moderate magnification, a dry objective will also work; the higher NA, the more spherical aberrations will be present. Keep in mind that when using a dry objective in immersion, the WD will increase (approximately by a factor equal to the RI of the new medium, eg. 17 mm in air will increase to 22.6 mm in water).
A detailed description of how an OpenSPIM can be upgraded to contain a second illumination axis with two cameras and multiple laser lines under the control of an Arduino board. The system also features a cooling chamber.