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Feature requests: (Un)trusted contigs and mixed read types support #49

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ohickl opened this issue Sep 13, 2024 · 0 comments
Open

Feature requests: (Un)trusted contigs and mixed read types support #49

ohickl opened this issue Sep 13, 2024 · 0 comments

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@ohickl
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ohickl commented Sep 13, 2024

Dear PenguiN team,

1. (Un)trusted-contigs option:
Have you considered adding an option to include existing contigs in the assembly process, alongside reads? Something like the (un)trusted-contigs option in SPAdes.
Could be useful for:

  • Including contigs from previous assemblies
  • Hybrid MG+MT datasets
  • Iterative assembly refinement

2. Mixing paired and single-end reads:
This doesn't seem possible at the moment, but you are already trying to merge paired reads internally in paired-end mode using Flash, as far as I understand. What happens with the non-overlapping pairs?
Could I just merge/interleave them in a similar way as done by PenguiN prior to running, concatenate with the single-end reads, and then run?
This would be particularly useful for pipelines dealing with datasets that include both types of reads, allowing utilization of all available data in a single assembly run.

Best regards,
Oskar

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