starfish is a Python library for processing images of image-based spatial transcriptomics. It lets you build scalable pipelines that localize and quantify RNA transcripts in image data generated by any FISH method, from simple RNA single-molecule FISH to combinatorial barcoded assays.
- Starfish enterprise: finding RNA patterns in single cells, Nature Technology Feature, Aug 2019
- Developing a Computational Pipeline and Benchmark Datasets for Image-Based Transcriptomics, ASCB Science News, Dec 2018
See spacetx-starfish.readthedocs.io for the quickstart, user guide, examples, and API.
starfish supports python 3.9-3.12 and can easily be installed from PyPI:
$ pip install starfish[napari]starfish will be dropping support for python 3.9 in future release when upstream dependencies will require a minimum python=3.10 version.
We welcome contributions from our users! See our contributing.rst and developer guide for more information.
- Forum: ask on the Image.sc forum
- Email: starfish@chanzuckerberg.com