From cf44db2901243dbc8e5e7253c28863c55fbb28aa Mon Sep 17 00:00:00 2001 From: Thu-Hien To Date: Thu, 10 Sep 2020 12:18:34 +0200 Subject: [PATCH] Allow bootstraps tree with different topology from input tree --- bin/lsd2_mac | Bin 507016 -> 515256 bytes bin/lsd2_unix | Bin 458200 -> 466336 bytes src/confidence_interval.cpp | 180 ++++++++++++++++++++++++------------ src/confidence_interval.h | 2 +- src/dating.cpp | 51 +++++----- src/options.cpp | 8 +- src/utils.cpp | 11 +++ 7 files changed, 165 insertions(+), 87 deletions(-) diff --git a/bin/lsd2_mac b/bin/lsd2_mac index 319df45ec9b880450749dbc255ef181ff9b54980..9e608c5098a1bac92082e473b05ee59e6328c005 100755 GIT binary patch delta 82659 zcmbrn34Bb~`#(PSCXrZTLKYEQ5X3GRq_%{FJ4`fcSJYC9Qc6-pB#;P|2muO z(aeR&Y3Ou*FF_p6a)5Y6ZKpt!LMX1?DN=XM%-6GNpzf-QnWjmAu2l(K7CqS{&%oeY z8q2pdmT^sgTi7R_p`CCs7#D+a%q_3e1wSsQ+v2Iy?NF#ZA%^dHoJHNQEKa;$|{lxH)av2>XKU-t5*WFyv7SEEs z!ab4$ME0FmQlQesTNernjX{m%}G2V#R|Sp82K;m9KpX1Y1(RWWA^~ 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b/src/confidence_interval.cpp @@ -169,7 +169,7 @@ void computeIC(double br,Pr* pr,Node** nodes,double* &T_left,double* &T_right,do delete[] HD_simul; } -void computeIC_bootstraps(InputOutputStream *io, Pr* pr,Node** nodes,double* &T_left,double* &T_right,double* &H_left,double* &H_right,double* &HD_left,double* &HD_right,double &rho_left,double& rho_right,double* &other_rhos_left,double* &other_rhos_right,int r){ +bool computeIC_bootstraps(InputOutputStream *io, Pr* pr,Node** nodes,double* &T_left,double* &T_right,double* &H_left,double* &H_right,double* &HD_left,double* &HD_right,double &rho_left,double& rho_right,double* &other_rhos_left,double* &other_rhos_right,int r){ int lineNb=getLineNumber(*(io->inBootstrapTree)); pr->nbBootstrap = lineNb; double** T_bootstrap = new double*[pr->nbBootstrap]; @@ -189,6 +189,7 @@ void computeIC_bootstraps(InputOutputStream *io, Pr* pr,Node** nodes,double* &T_ extrait_outgroup(io, pr, true); } bool constraintConsistent=true; + bool sameTopology = true; for (int y = 0; y< pr->nbBootstrap; y++){ pr->internalConstraints.clear(); Node** nodes_bootstrap=tree2data(*(io->inBootstrapTree),pr,s); @@ -223,7 +224,7 @@ void computeIC_bootstraps(InputOutputStream *io, Pr* pr,Node** nodes,double* &T_ initConstraint(pr, nodes_bootstrap); if (pr->e>0) remove_outlier_nodes(pr,nodes_bootstrap); if ((original_nbInodes != pr->nbINodes) || (original_nbBranches != pr->nbBranches) || !checkTopology(pr,nodes,nodes_bootstrap)){ - myExit("The bootstrap tree %d does not have the same topology as the original tree\n",y+1); + sameTopology = false; } if (!pr->constraint){//LD without constraints if (pr->estimate_root==""){ @@ -246,14 +247,19 @@ void computeIC_bootstraps(InputOutputStream *io, Pr* pr,Node** nodes,double* &T_ if (pr->verbose){ cout<<"Tree "<rho<<", rMRCA: "<D<nbBranches+1]; - H_bootstrap[y] = new double[pr->nbBranches+1]; - HD_bootstrap[y] = new double[pr->nbBranches+1]; - calculate_tree_height(pr,nodes_bootstrap); - for (int i=0;i<=pr->nbBranches;i++){ - T_bootstrap[y][i] = nodes_bootstrap[i]->D; - H_bootstrap[y][i] = nodes_bootstrap[i]->H; - HD_bootstrap[y][i] = nodes_bootstrap[i]->HD; + if (sameTopology){ + T_bootstrap[y] = new double[pr->nbBranches+1]; + H_bootstrap[y] = new double[pr->nbBranches+1]; + HD_bootstrap[y] = new double[pr->nbBranches+1]; + calculate_tree_height(pr,nodes_bootstrap); + for (int i=0;i<=pr->nbBranches;i++){ + T_bootstrap[y][i] = nodes_bootstrap[i]->D; + H_bootstrap[y][i] = nodes_bootstrap[i]->H; + HD_bootstrap[y][i] = nodes_bootstrap[i]->HD; + } + } else{ + T_bootstrap[y] = new double[1]; + T_bootstrap[y][0] = nodes_bootstrap[0]->D; } rho_bootstrap[y] = pr->rho; other_rhos_bootstrap[y] = new double[pr->ratePartition.size()]; @@ -264,39 +270,61 @@ void computeIC_bootstraps(InputOutputStream *io, Pr* pr,Node** nodes,double* &T_ delete[] nodes_bootstrap; } pr->objective = original_objective; + if (!sameTopology){ + cout<<"At least one bootstrap trees do not have the same topology as the input tree."<warningMessage.push_back(oss.str()); + } + if (sameTopology){ + double* T_sort = new double[pr->nbBootstrap]; + double* H_sort = new double[pr->nbBootstrap]; + double* HD_sort = new double[pr->nbBootstrap]; + for (int i=0;i<=pr->nbBranches;i++){ + for (int j=0;jnbBootstrap;j++) { + T_sort[j]=T_bootstrap[j][i]; + H_sort[j]=H_bootstrap[j][i]; + HD_sort[j]=HD_bootstrap[j][i]; + } + sort(T_sort,pr->nbBootstrap); + sort(H_sort,pr->nbBootstrap); + sort(HD_sort,pr->nbBootstrap); + + T_left[i]=T_sort[int(0.025*pr->nbBootstrap)]; + if (T_left[i]>nodes[i]->D) T_left[i]=nodes[i]->D; + T_right[i]=T_sort[pr->nbBootstrap-int(0.025*pr->nbBootstrap)-1]; + if (T_right[i]D) T_right[i]=nodes[i]->D; + + H_left[i]=H_sort[int(0.025*pr->nbBootstrap)]; + if (H_left[i]>nodes[i]->H) H_left[i]=nodes[i]->H; + H_right[i]=H_sort[pr->nbBootstrap-int(0.025*pr->nbBootstrap)-1]; + if (H_right[i]H) H_right[i]=nodes[i]->H; + + HD_left[i]=HD_sort[int(0.025*pr->nbBootstrap)]; + if (HD_left[i]>nodes[i]->HD) HD_left[i]=nodes[i]->HD; + HD_right[i]=HD_sort[pr->nbBootstrap-int(0.025*pr->nbBootstrap)-1]; + if (HD_right[i]HD) HD_right[i]=nodes[i]->HD; + } + delete[] T_sort; + delete[] H_sort; + delete[] HD_sort; + } else{ + double* T_sort = new double[pr->nbBootstrap]; + for (int i=0; inbBootstrap; i++){ + T_sort[i] = T_bootstrap[i][0]; + } + sort(T_sort,pr->nbBootstrap); + T_left[0]=T_sort[int(0.025*pr->nbBootstrap)]; + if (T_left[0]>nodes[0]->D) T_left[0]=nodes[0]->D; + T_right[0]=T_sort[pr->nbBootstrap-int(0.025*pr->nbBootstrap)-1]; + if (T_right[0]D) T_right[0]=nodes[0]->D; + delete[] T_sort; + } sort(rho_bootstrap,pr->nbBootstrap); rho_left=rho_bootstrap[int(0.025*pr->nbBootstrap)]; rho_right=rho_bootstrap[pr->nbSampling-int(0.025*pr->nbBootstrap)-1]; if (pr->rhorho; if (pr->rho>rho_right) rho_right=pr->rho; - double* T_sort = new double[pr->nbBootstrap]; - double* H_sort = new double[pr->nbBootstrap]; - double* HD_sort = new double[pr->nbBootstrap]; - for (int i=0;i<=pr->nbBranches;i++){ - for (int j=0;jnbBootstrap;j++) { - T_sort[j]=T_bootstrap[j][i]; - H_sort[j]=H_bootstrap[j][i]; - HD_sort[j]=HD_bootstrap[j][i]; - } - sort(T_sort,pr->nbBootstrap); - sort(H_sort,pr->nbBootstrap); - sort(HD_sort,pr->nbBootstrap); - - T_left[i]=T_sort[int(0.025*pr->nbBootstrap)]; - if (T_left[i]>nodes[i]->D) T_left[i]=nodes[i]->D; - T_right[i]=T_sort[pr->nbBootstrap-int(0.025*pr->nbBootstrap)-1]; - if (T_right[i]D) T_right[i]=nodes[i]->D; - - H_left[i]=H_sort[int(0.025*pr->nbBootstrap)]; - if (H_left[i]>nodes[i]->H) H_left[i]=nodes[i]->H; - H_right[i]=H_sort[pr->nbBootstrap-int(0.025*pr->nbBootstrap)-1]; - if (H_right[i]H) H_right[i]=nodes[i]->H; - - HD_left[i]=HD_sort[int(0.025*pr->nbBootstrap)]; - if (HD_left[i]>nodes[i]->HD) HD_left[i]=nodes[i]->HD; - HD_right[i]=HD_sort[pr->nbBootstrap-int(0.025*pr->nbBootstrap)-1]; - if (HD_right[i]HD) HD_right[i]=nodes[i]->HD; - } double* other_rhos_bootstrap_sort = new double[pr->nbBootstrap]; for (int g=1;g<=pr->ratePartition.size();g++){ for (int r=0;rnbBootstrap;r++) { @@ -309,30 +337,24 @@ void computeIC_bootstraps(InputOutputStream *io, Pr* pr,Node** nodes,double* &T_ if (other_rhos_right[g]rho*pr->multiplierRate[g]) other_rhos_right[g]=pr->rho*pr->multiplierRate[g]; } delete[] rho_bootstrap; - delete[] T_sort; - delete[] H_sort; - delete[] HD_sort; delete[] other_rhos_bootstrap_sort; delete[] other_rhos_bootstrap; + if (sameTopology){ + for (int i=0;inbBootstrap;i++){ + delete[] H_bootstrap[i]; + delete[] HD_bootstrap[i]; + } + } for (int i=0;inbBootstrap;i++){ delete[] T_bootstrap[i]; - delete[] H_bootstrap[i]; - delete[] HD_bootstrap[i]; } delete[] T_bootstrap; delete[] H_bootstrap; delete[] HD_bootstrap; + return sameTopology; } void output(double br,int y, InputOutputStream *io, Pr* pr,Node** nodes,ostream& f,ostream& tree1,ostream& tree2,ostream& tree3,int r){ - /*if (pr->outlier.size()>0){ - std::ostringstream oss; - oss<<"- There are "<outlier.size()<<" nodes that are considered as outliers and were excluded from the analysis:\n"; - for (int i=0;ioutlier.size();i++){ - oss<<" "<outlier[i]]->L<<", suggesting date "<outlier[i]]->D<<"\n"; - } - pr->resultMessage.push_back(oss.str()); - }*/ if (!pr->constraint && pr->ci) { std::ostringstream oss; oss<<"- Confidence intervals are not warranted under non-constraint mode.\n"; @@ -351,8 +373,19 @@ void output(double br,int y, InputOutputStream *io, Pr* pr,Node** nodes,ostream& oss<<"- Dating results:\n"; oss<<" rate "<rho<<", tMRCA "<objective<<"\n"; pr->resultMessage.push_back(oss.str()); - } - else{ + } else if (pr->splitExternal){ + std::ostringstream oss; + oss<<"- Dating results:\n"; + if (pr->multiplierRate[0]!=-1){ + oss<<" rate internalBranches "<rho<<", "; + } + for (int i=1; i<=pr->ratePartition.size(); i++) { + if (pr->multiplierRate[i]>0) + oss<<"rate "<ratePartition[i-1]->name.c_str()<<" "<rho*pr->multiplierRate[i]<<", "; + } + oss<<"tMRCA "<objective<<"\n"; + pr->resultMessage.push_back(oss.str()); + } else{ std::ostringstream oss; oss<<"- Dating results:\n"; if (pr->multiplierRate[0]!=-1){ @@ -402,8 +435,18 @@ void output(double br,int y, InputOutputStream *io, Pr* pr,Node** nodes,ostream& std::ostringstream oss; oss<<" rate "<rho<<", tMRCA "<objective<<"\n"; pr->resultMessage.push_back(oss.str()); - } - else{ + } else if (pr->splitExternal){ + std::ostringstream oss; + if (pr->multiplierRate[0]!=-1){ + oss<<" rate internal Branches "<rho<<", "; + } + for (int i=1; i<=pr->ratePartition.size(); i++) { + if (pr->multiplierRate[i]>0) + oss<<"rate "<ratePartition[i-1]->name.c_str()<<" "<rho*pr->multiplierRate[i]<<", "; + } + oss<<"tMRCA "<objective<<"\n"; + pr->resultMessage.push_back(oss.str()); + } else{ std::ostringstream oss; if (pr->multiplierRate[0]!=-1){ oss<<" rate "<rho<<", "; @@ -468,12 +511,13 @@ void output(double br,int y, InputOutputStream *io, Pr* pr,Node** nodes,ostream& double rho_left,rho_right; double* other_rhos_left = new double[pr->ratePartition.size()+1]; double* other_rhos_right = new double[pr->ratePartition.size()+1]; + bool sameTopology = true; if (pr->bootstraps_file==""){ cout<<"Computing confidence intervals using sequence length "<seqLength<<" and a lognormal\n relaxed clock with mean 1, standard deviation "<q<<" (settable via option -q)"<rho<<" ["<objective<<"\n"; pr->resultMessage.push_back(oss.str()); - } - else{ + } else if (pr->splitExternal){ + std::ostringstream oss; + if (pr->multiplierRate[0]!=-1){ + oss<<" rate internalBranches "<rho<<" ["<ratePartition.size(); i++) { + if (pr->multiplierRate[i]>0) oss<<"rate "<ratePartition[i-1]->name.c_str()<<" "<rho*pr->multiplierRate[i]<<" ["<objective<<"\n"; + pr->resultMessage.push_back(oss.str()); + } else{ std::ostringstream oss; if (pr->multiplierRate[0]!=-1){ oss<<" rate "<rho<<" ["<resultMessage.size();i++){ diff --git a/src/confidence_interval.h b/src/confidence_interval.h index 7a0dbbf..f8f4760 100644 --- a/src/confidence_interval.h +++ b/src/confidence_interval.h @@ -9,6 +9,6 @@ using namespace std; void computeIC(double br,Pr* pr,Node** nodes,double* &T_left,double* &T_right,double* &H_left,double* &H_right,double* &HD_left,double* &HD_right,double &rho_left,double& rho_right,double* &other_rhos_left,double* &other_rhos_right); -void computeIC_bootstraps(InputOutputStream *io, Pr* pr,Node** nodes,double* &T_left,double* &T_right,double* &H_left,double* &H_right,double* &HD_left,double* &HD_right,double &rho_left,double& rho_right,double* &other_rhos_left,double* &other_rhos_right,int r); +bool computeIC_bootstraps(InputOutputStream *io, Pr* pr,Node** nodes,double* &T_left,double* &T_right,double* &H_left,double* &H_right,double* &HD_left,double* &HD_right,double &rho_left,double& rho_right,double* &other_rhos_left,double* &other_rhos_right,int r); void output(double br,int y, InputOutputStream *io, Pr* pr,Node** nodes,ostream& f,ostream& tree1,ostream& tree2,ostream& tree3,int r); diff --git a/src/dating.cpp b/src/dating.cpp index a8bd5a7..7814e9e 100644 --- a/src/dating.cpp +++ b/src/dating.cpp @@ -277,7 +277,7 @@ bool without_constraint_active_set(Pr* pr,Node** nodes){ for (int i=0; i<=pr->nbBranches; i++) { D_old[i]=nodes[i]->D; } - double val = without_constraint(pr,nodes); + bool val = without_constraint(pr,nodes); if (!val) { cerr<<"Error: There's not enough signal in the input temporal constraints to have unique solution."<V = V[r]/m/m; } } - if (pr->verbose){ - for (int i=1;imultiplierRate.size();i++) cout<multiplierRate[i]<<" "; - } bool val = without_constraint_active_set(pr,nodes); if (!val) return false; if (pr->ratePartition.size()>0) { + if (pr->verbose){ + printf("ROUND 0 , objective function %.15e , rate %.15f , other_rates ",pr->objective,pr->rho); + for (int r=1; r<=pr->ratePartition.size(); r++) { + printf(" %.15f",pr->rho*pr->multiplierRate[r]); + } + cout<ratePartition.size()+1]; @@ -979,6 +983,14 @@ bool without_constraint_multirates(Pr* pr,Node** nodes,bool reassign){ for (int r=1; r<=pr->ratePartition.size(); r++) { cont = cont || (pr->multiplierRate[r]>0 && abs((old_multi[r]*old_rho-pr->multiplierRate[r]*pr->rho)/pr->multiplierRate[r]/pr->rho)>=1e-5); } + if (pr->verbose){ + printf("ROUND %d , objective function %.15e , rate %.15f , other_rates ",i,pr->objective,pr->rho); + for (int r=1; r<=pr->ratePartition.size(); r++) { + printf(" %.15f",pr->rho*pr->multiplierRate[r]); + } + printf(", diff %.15f",abs((old_rho-pr->rho)/pr->rho)); + cout<nbBranches; i++) { @@ -1006,16 +1018,15 @@ bool with_constraint_multirates(Pr* pr,Node** nodes,bool reassign){ nodes[r]->V = V[r]/m/m; } } - if (pr->verbose) { - for (int i=1;imultiplierRate.size();i++) cout<multiplierRate[i]<<" "; - } bool val = with_constraint_active_set(pr,nodes); if (pr->ratePartition.size()>0) { - /*printf("ROUND 0 , objective function %.15e , rate %.15f ",pr->objective,pr->rho); - for (int r=1; r<=pr->ratePartition.size(); r++) { - if (!nan[r]) printf(" %.15f ",pr->rho*pr->multiplierRate[r]); + if (pr->verbose){ + printf("ROUND 0 , objective function %.15e , rate %.15f , other_rates ",pr->objective,pr->rho); + for (int r=1; r<=pr->ratePartition.size(); r++) { + printf(" %.15f",pr->rho*pr->multiplierRate[r]); + } + cout<ratePartition.size()+1]; @@ -1057,22 +1068,20 @@ bool with_constraint_multirates(Pr* pr,Node** nodes,bool reassign){ for (int r=1; r<=pr->ratePartition.size(); r++) { cont = cont || (pr->multiplierRate[r]>0 && abs((old_multi[r]*old_rho-pr->multiplierRate[r]*pr->rho)/pr->multiplierRate[r]/pr->rho)>=1e-5); } - /*printf("ROUND %d , objective function %.15e , rate %.15f ",i,pr->objective,pr->rho); - for (int r=1; r<=pr->ratePartition.size(); r++) { - if (!nan[r]) printf(" %.15f ",pr->rho*pr->multiplierRate[r]); + if (pr->verbose){ + printf("ROUND %d , objective function %.15e , rate %.15f , other_rates ",i,pr->objective,pr->rho); + for (int r=1; r<=pr->ratePartition.size(); r++) { + printf(" %.15f",pr->rho*pr->multiplierRate[r]); + } + printf(", diff %.15f",abs((old_rho-pr->rho)/pr->rho)); + cout<nbBranches; r++) { nodes[r]->B = B[r]; nodes[r]->V = V[r]; } - /*printf("ROUND %d , objective function %.15e , rate %.15f ",i,pr->objective,pr->rho); - for (int r=1; r<=pr->ratePartition.size(); r++) { - if (pr->multiplierRate[r]>0) printf(" %.15f ",pr->rho*pr->multiplierRate[r]); - } - cout<nbBranches+1]; + for (int i=pr->nbINodes;i<=pr->nbBranches;i++){ + if (nodes1[i]->L != nodes2[i]->L) return false; + } + for (int i=1;i<=pr->nbBranches;i++){ + if (nodes1[i]->P != nodes2[i]->P) return false; + } + return true; +}*/