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Will I get the same results if I re-run Prokka 1.14.5 on the same input files? #714

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PollyHannah opened this issue Dec 2, 2024 · 0 comments

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@PollyHannah
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Hey! I'm using Prokka 1.14.5 to annotate a bunch of virus genomes using the virus database (code below).

Every now and again I find a new a genome to annotate. If I re-run Prokka 1.14.5 on the new and old genome files, will the annotations and locus tags for the genomes I have already re-annotated, match the output from the last time I ran it?

I'm worried Prokka is pulling data from a database which is always updating which means if i run the same script on the same genome like 3 weeks later it the output might be different..

GENOMES=(ls ./genomes_1/*.fasta)

for INPUT in ${GENOMES[@]} ; do
File=(basename ${INPUT} .fasta)
prokka ${INPUT} --kingdom viruses --outdir ./prokka_outputs/${File} --prefix ${File} --locustag ${File}
done

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