diff --git a/tests/drivers/i2c/i2c_target_api/boards/nucleo_f746zg.conf b/tests/drivers/i2c/i2c_target_api/boards/nucleo_f746zg.conf
new file mode 100644
index 00000000000000..34b2571d125164
--- /dev/null
+++ b/tests/drivers/i2c/i2c_target_api/boards/nucleo_f746zg.conf
@@ -0,0 +1,2 @@
+CONFIG_I2C_STM32_INTERRUPT=y
+CONFIG_I2C_VIRTUAL=n
diff --git a/tests/drivers/i2c/i2c_target_api/boards/nucleo_f746zg.overlay b/tests/drivers/i2c/i2c_target_api/boards/nucleo_f746zg.overlay
new file mode 100644
index 00000000000000..8e740ab17af7cd
--- /dev/null
+++ b/tests/drivers/i2c/i2c_target_api/boards/nucleo_f746zg.overlay
@@ -0,0 +1,30 @@
+/*
+ * Copyright (c) 2020 Nordic Semiconductor ASA
+ * SPDX-License-Identifier: Apache-2.0
+ */
+
+/* I2C bus pins are exposed on the ST morpho header.
+ *
+ *  Bus        SDA               SCL
+ *          Pin   Hdr         Pin   Hdr
+ *  i2c1    PB9   CN7:4      PB8   CN7:2
+ *  i2c2    PB11  CN10:34    PB10  CN10:32
+ *
+ * Short Pin PB9 to PB11, and PB8 to PB10, for the test to pass.
+ */
+
+&i2c1 {
+	eeprom0: eeprom@54 {
+		compatible = "zephyr,i2c-target-eeprom";
+		reg = <0x54>;
+		size = <1024>;
+	};
+};
+
+&i2c2 {
+	eeprom1: eeprom@56 {
+		compatible = "zephyr,i2c-target-eeprom";
+		reg = <0x56>;
+		size = <1024>;
+	};
+};