diff --git a/samples/bluetooth/hci_ipc/boards/nrf5340_audio_dk_nrf5340_cpunet_nrf21540_ek.overlay b/samples/bluetooth/hci_ipc/boards/nrf5340_audio_dk_nrf5340_cpunet_nrf21540_ek.overlay
index cb056659aafaf8..82e6b8cf848564 100644
--- a/samples/bluetooth/hci_ipc/boards/nrf5340_audio_dk_nrf5340_cpunet_nrf21540_ek.overlay
+++ b/samples/bluetooth/hci_ipc/boards/nrf5340_audio_dk_nrf5340_cpunet_nrf21540_ek.overlay
@@ -6,23 +6,10 @@
 		pdn-gpios         = <&arduino_header 15 GPIO_ACTIVE_HIGH>; /* D9 */
 		ant-sel-gpios     = <&arduino_header 10 GPIO_ACTIVE_HIGH>; /* D4 */
 		mode-gpios        = <&arduino_header 8  GPIO_ACTIVE_HIGH>; /* D2 */
-		spi-if            = <&nrf_radio_fem_spi>;
 		supply-voltage-mv = <3000>;
 	};
 };
 
-&spi0 {
-	/* status   = "okay"; */
-	cs-gpios = <&arduino_header 16 GPIO_ACTIVE_LOW>; /* D10 */
-
-	nrf_radio_fem_spi: nrf21540_fem_spi@0 {
-		compatible        = "nordic,nrf21540-fem-spi";
-		/* status            = "okay"; */
-		reg               = <0>;
-		spi-max-frequency = <8000000>;
-	};
-};
-
 &radio {
 	fem = <&nrf_radio_fem>;
 };
diff --git a/subsys/bluetooth/controller/ll_sw/nordic/hal/nrf5/radio/radio.c b/subsys/bluetooth/controller/ll_sw/nordic/hal/nrf5/radio/radio.c
index 8ec0e62fa50d5c..79954af8da295c 100644
--- a/subsys/bluetooth/controller/ll_sw/nordic/hal/nrf5/radio/radio.c
+++ b/subsys/bluetooth/controller/ll_sw/nordic/hal/nrf5/radio/radio.c
@@ -79,9 +79,6 @@ BUILD_ASSERT(NRF_DT_GPIOTE_INST(FEM_NODE, HAL_RADIO_GPIO_PA_PROP) ==
 static const nrfx_gpiote_t gpiote_pdn = NRFX_GPIOTE_INSTANCE(
 	NRF_DT_GPIOTE_INST(FEM_NODE, pdn_gpios));
 static uint8_t gpiote_ch_pdn;
-static const nrfx_gpiote_t gpiote_csn = NRFX_GPIOTE_INSTANCE(
-	NRF_DT_GPIOTE_INST(DT_BUS(FEM_SPI_DEV_NODE), cs_gpios));
-static uint8_t gpiote_ch_csn;
 #endif
 
 /* These headers require the above gpiote-related variables to be declared. */
@@ -134,20 +131,6 @@ BUILD_ASSERT(!BAD_FLAGS(NRF_GPIO_LNA_FLAGS),
 NRF_DT_CHECK_GPIO_CTLR_IS_SOC(FEM_NODE, pdn_gpios, "pdn-gpios");
 #endif	/* DT_NODE_HAS_PROP(FEM_NODE, pdn_gpios) */
 
-/* CSN is special because it comes from the spi-if property. */
-#if defined(HAL_RADIO_FEM_NRF21540_HAS_CSN)
-#define NRF_GPIO_CSN_CTLR  DT_SPI_DEV_CS_GPIOS_CTLR(FEM_SPI_DEV_NODE)
-#define NRF_GPIO_CSN       ((NRF_GPIO_Type *)DT_REG_ADDR(NRF_GPIO_CSN_CTLR))
-#define NRF_GPIO_CSN_PIN   DT_SPI_DEV_CS_GPIOS_PIN(FEM_SPI_DEV_NODE)
-#define NRF_GPIO_CSN_FLAGS DT_SPI_DEV_CS_GPIOS_FLAGS(FEM_SPI_DEV_NODE)
-#define NRF_GPIO_CSN_PSEL  (NRF_GPIO_CSN_PIN + \
-			    (DT_PROP(NRF_GPIO_CSN_CTLR, port) << 5))
-BUILD_ASSERT(DT_NODE_HAS_COMPAT(NRF_GPIO_CSN_CTLR, nordic_nrf_gpio),
-	     "fem node " DT_NODE_PATH(FEM_NODE) " has a spi-if property, "
-	     " but the chip select pin is not on the SoC. Check cs-gpios in "
-	     DT_NODE_PATH(DT_BUS(FEM_SPI_DEV_NODE)));
-#endif	/* HAL_RADIO_FEM_NRF21540_HAS_CSN */
-
 #endif	/* HAL_RADIO_FEM_IS_NRF21540 */
 
 /* CTEINLINE S0_MASK for periodic advertising PUDs. It allows to accept all types of extended
@@ -208,15 +191,6 @@ void radio_setup(void)
 	}
 #endif /* NRF_GPIO_PDN_PIN */
 
-#if defined(NRF_GPIO_CSN_PIN)
-	NRF_GPIO_CSN->DIRSET = BIT(NRF_GPIO_CSN_PIN);
-	if (ACTIVE_LOW(NRF_GPIO_CSN_FLAGS)) {
-		NRF_GPIO_CSN->OUTSET = BIT(NRF_GPIO_CSN_PIN);
-	} else {
-		NRF_GPIO_CSN->OUTCLR = BIT(NRF_GPIO_CSN_PIN);
-	}
-#endif /* NRF_GPIO_CSN_PIN */
-
 	hal_radio_ram_prio_setup();
 }
 
@@ -1841,12 +1815,6 @@ int radio_gpio_pa_lna_init(void)
 	}
 #endif
 
-#if defined(NRF_GPIO_CSN_PIN)
-	if (nrfx_gpiote_channel_alloc(&gpiote_csn, &gpiote_ch_csn) != NRFX_SUCCESS) {
-		return -ENOMEM;
-	}
-#endif
-
 	return 0;
 }
 
@@ -1859,10 +1827,6 @@ void radio_gpio_pa_lna_deinit(void)
 #if defined(NRF_GPIO_PDN_PIN)
 	(void)nrfx_gpiote_channel_free(&gpiote_pdn, gpiote_ch_pdn);
 #endif
-
-#if defined(NRF_GPIO_CSN_PIN)
-	(void)nrfx_gpiote_channel_free(&gpiote_csn, gpiote_ch_csn);
-#endif
 }
 
 #if defined(HAL_RADIO_GPIO_HAVE_PA_PIN)
@@ -1881,7 +1845,6 @@ void radio_gpio_pa_setup(void)
 #if defined(HAL_RADIO_FEM_IS_NRF21540)
 	hal_pa_ppi_setup();
 	radio_gpio_pdn_setup();
-	radio_gpio_csn_setup();
 #endif
 }
 #endif /* HAL_RADIO_GPIO_HAVE_PA_PIN */
@@ -1902,7 +1865,6 @@ void radio_gpio_lna_setup(void)
 #if defined(HAL_RADIO_FEM_IS_NRF21540)
 	hal_lna_ppi_setup();
 	radio_gpio_pdn_setup();
-	radio_gpio_csn_setup();
 #endif
 }
 
@@ -1922,22 +1884,6 @@ void radio_gpio_pdn_setup(void)
 #endif /* NRF_GPIO_PDN_PIN */
 }
 
-void radio_gpio_csn_setup(void)
-{
-	/* Note: the spi-if property is optional. */
-#if defined(NRF_GPIO_CSN_PIN)
-	gpiote_csn.p_reg->CONFIG[gpiote_ch_csn] =
-		(GPIOTE_CONFIG_MODE_Task <<
-		 GPIOTE_CONFIG_MODE_Pos) |
-		(NRF_GPIO_CSN_PSEL <<
-		 GPIOTE_CONFIG_PSEL_Pos) |
-		(GPIOTE_CONFIG_POLARITY_Toggle <<
-		 GPIOTE_CONFIG_POLARITY_Pos) |
-		(OUTINIT_INACTIVE(NRF_GPIO_CSN_FLAGS) <<
-		 GPIOTE_CONFIG_OUTINIT_Pos);
-#endif /* NRF_GPIO_CSN_PIN */
-}
-
 void radio_gpio_lna_on(void)
 {
 	if (ACTIVE_LOW(NRF_GPIO_LNA_FLAGS)) {
@@ -1981,7 +1927,6 @@ void radio_gpio_pa_lna_disable(void)
 					   BIT(HAL_DISABLE_FEM_PPI));
 	gpiote_palna.p_reg->CONFIG[gpiote_ch_palna] = 0;
 	gpiote_pdn.p_reg->CONFIG[gpiote_ch_pdn] = 0;
-	gpiote_csn.p_reg->CONFIG[gpiote_ch_csn] = 0;
 #else
 	hal_radio_nrf_ppi_channels_disable(BIT(HAL_ENABLE_PALNA_PPI) |
 					   BIT(HAL_DISABLE_PALNA_PPI));
diff --git a/subsys/bluetooth/controller/ll_sw/nordic/hal/nrf5/radio/radio.h b/subsys/bluetooth/controller/ll_sw/nordic/hal/nrf5/radio/radio.h
index 3d517121ec0414..eac3381c8824de 100644
--- a/subsys/bluetooth/controller/ll_sw/nordic/hal/nrf5/radio/radio.h
+++ b/subsys/bluetooth/controller/ll_sw/nordic/hal/nrf5/radio/radio.h
@@ -163,7 +163,6 @@ void radio_gpio_pa_lna_deinit(void);
 void radio_gpio_pa_setup(void);
 void radio_gpio_lna_setup(void);
 void radio_gpio_pdn_setup(void);
-void radio_gpio_csn_setup(void);
 void radio_gpio_lna_on(void);
 void radio_gpio_lna_off(void);
 void radio_gpio_pa_lna_enable(uint32_t trx_us);
diff --git a/subsys/bluetooth/controller/ll_sw/nordic/hal/nrf5/radio/radio_nrf5_dppi_gpiote.h b/subsys/bluetooth/controller/ll_sw/nordic/hal/nrf5/radio/radio_nrf5_dppi_gpiote.h
index 1403921ac22c50..428bcb869687b8 100644
--- a/subsys/bluetooth/controller/ll_sw/nordic/hal/nrf5/radio/radio_nrf5_dppi_gpiote.h
+++ b/subsys/bluetooth/controller/ll_sw/nordic/hal/nrf5/radio/radio_nrf5_dppi_gpiote.h
@@ -66,10 +66,6 @@ static inline void hal_fem_ppi_setup(void)
 	hal_gpiote_tasks_setup(gpiote_pdn.p_reg, gpiote_ch_pdn,
 			       IS_ENABLED(HAL_RADIO_GPIO_NRF21540_PDN_POL_INV),
 			       HAL_ENABLE_FEM_PPI, HAL_DISABLE_FEM_PPI);
-
-	hal_gpiote_tasks_setup(gpiote_csn.p_reg, gpiote_ch_csn,
-			       IS_ENABLED(HAL_RADIO_GPIO_NRF21540_CSN_POL_INV),
-			       HAL_ENABLE_FEM_PPI, HAL_DISABLE_FEM_PPI);
 }
 
 #endif /* HAL_RADIO_FEM_IS_NRF21540 */
diff --git a/subsys/bluetooth/controller/ll_sw/nordic/hal/nrf5/radio/radio_nrf5_fem_nrf21540.h b/subsys/bluetooth/controller/ll_sw/nordic/hal/nrf5/radio/radio_nrf5_fem_nrf21540.h
index 6b473198ffaf48..3ecefdb422d0ca 100644
--- a/subsys/bluetooth/controller/ll_sw/nordic/hal/nrf5/radio/radio_nrf5_fem_nrf21540.h
+++ b/subsys/bluetooth/controller/ll_sw/nordic/hal/nrf5/radio/radio_nrf5_fem_nrf21540.h
@@ -59,19 +59,3 @@
 #define HAL_RADIO_GPIO_NRF21540_PDN_POL_INV 1
 #endif	/* DT_GPIO_FLAGS(FEM_NODE, pdn_gpios) & GPIO_ACTIVE_LOW */
 #endif	/* FEM_HAS_PROP(pdn_gpios) */
-
-#if FEM_HAS_PROP(spi_if)
-/* This is the "SPI device" node, i.e. the one with compatible
- * nordic,nrf21540-fem-spi.
- */
-#define FEM_SPI_DEV_NODE DT_PHANDLE(FEM_NODE, spi_if)
-/* If the SPI device node has a chip select gpio... */
-#if DT_SPI_DEV_HAS_CS_GPIOS(FEM_SPI_DEV_NODE)
-/* set a macro indicating that, and... */
-#define HAL_RADIO_FEM_NRF21540_HAS_CSN 1
-/* use it to get the CSN polarity. */
-#if DT_SPI_DEV_CS_GPIOS_FLAGS(FEM_SPI_DEV_NODE) & GPIO_ACTIVE_LOW
-#define HAL_RADIO_GPIO_NRF21540_CSN_POL_INV 1
-#endif	/* DT_SPI_DEV_CS_GPIOS_FLAGS(FEM_SPI_DEV_NODE) & GPIO_ACTIVE_LOW */
-#endif	/* DT_SPI_DEV_HAS_CS_GPIOS(FEM_SPI_DEV_NODE) */
-#endif	/* FEM_HAS_PROP(spi_if) */
diff --git a/subsys/bluetooth/controller/ll_sw/nordic/hal/nrf5/radio/radio_nrf5_ppi_gpiote.h b/subsys/bluetooth/controller/ll_sw/nordic/hal/nrf5/radio/radio_nrf5_ppi_gpiote.h
index ec3c11fea1b362..268bae5aef177f 100644
--- a/subsys/bluetooth/controller/ll_sw/nordic/hal/nrf5/radio/radio_nrf5_ppi_gpiote.h
+++ b/subsys/bluetooth/controller/ll_sw/nordic/hal/nrf5/radio/radio_nrf5_ppi_gpiote.h
@@ -36,18 +36,16 @@ static inline void hal_lna_ppi_setup(void)
 
 static inline void hal_fem_ppi_setup(void)
 {
-	nrf_ppi_channel_and_fork_endpoint_setup(
+	nrf_ppi_channel_endpoint_setup(
 		NRF_PPI,
 		HAL_ENABLE_FEM_PPI,
 		(uint32_t)&(EVENT_TIMER->EVENTS_COMPARE[3]),
-		(uint32_t)&(gpiote_pdn.p_reg->TASKS_OUT[gpiote_ch_pdn]),
-		(uint32_t)&(gpiote_csn.p_reg->TASKS_OUT[gpiote_ch_csn]));
-	nrf_ppi_channel_and_fork_endpoint_setup(
+		(uint32_t)&(gpiote_pdn.p_reg->TASKS_OUT[gpiote_ch_pdn]));
+	nrf_ppi_channel_endpoint_setup(
 		NRF_PPI,
 		HAL_DISABLE_FEM_PPI,
 		(uint32_t)&(NRF_RADIO->EVENTS_DISABLED),
-		(uint32_t)&(gpiote_pdn.p_reg->TASKS_OUT[gpiote_ch_pdn]),
-		(uint32_t)&(gpiote_csn.p_reg->TASKS_OUT[gpiote_ch_csn]));
+		(uint32_t)&(gpiote_pdn.p_reg->TASKS_OUT[gpiote_ch_pdn]));
 }
 
 #endif /* HAL_RADIO_FEM_IS_NRF21540 */