diff --git a/samples/drivers/uart/native_tty/boards/native_sim.overlay b/samples/drivers/uart/native_tty/boards/native_sim.overlay
new file mode 100644
index 000000000000000..24f1bd95705bda3
--- /dev/null
+++ b/samples/drivers/uart/native_tty/boards/native_sim.overlay
@@ -0,0 +1,15 @@
+/ {
+	uart0: uart {
+		status = "okay";
+		compatible = "zephyr,native-tty-uart";
+		current-speed = <115200>;
+		serial-port = "/dev/ttyUSB0";
+	};
+
+	uart2: uart2 {
+		status = "okay";
+		compatible = "zephyr,native-tty-uart";
+		current-speed = <115200>;
+		serial-port = "/dev/ttyUSB1";
+	};
+};
diff --git a/samples/drivers/uart/native_tty/sample.yaml b/samples/drivers/uart/native_tty/sample.yaml
index 92263aa8c426a5c..1e059d9ce3aab39 100644
--- a/samples/drivers/uart/native_tty/sample.yaml
+++ b/samples/drivers/uart/native_tty/sample.yaml
@@ -3,7 +3,9 @@ sample:
 tests:
   sample.drivers.uart.native_tty:
     build_only: true
-    platform_allow: native_posix
+    platform_allow:
+      - native_posix
+      - native_sim
     tags:
       - serial
       - uart