A tool for data evaluation in perpendicular line scanning fluorescence correlation spectroscopy (FCS)
When a membrane is scanned perpendicularly to its surface, the fluorescence signal originating from the membrane itself must be separated from the signal of the surrounding medium for an FCS analysis. PyScanFCS interactively extracts the fluctuating fluorescence signal from such measurements and applies a multiple-tau algorithm. The obtained correlation curves can be evaluated using PyCorrFit.
Installers for PyScanFCS are available at the release page.
Alternatively, install with pip install pyscanfcs.
A detailed documentation including an explanation of the graphical user interface and available model functions is available as a PDF file.
