Supported Bioinformatic Analysis Softwares

Index

• 1、BWA for gtz

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1、BWA for gtz

• How to Install?

  For installation you can (recommended)

  sudo curl -sSL https://gtz.io/bwagtz_latest.run -o /tmp/bwagtz.run && sudo sh /tmp/bwagtz.run

  or

  download installation files：-GTX.Zip bwa-gtz-
  Run commands in the installation file directory

  sudo sh bwagtz_lastest.run

  complete installation according to prompt.

• How to Use?

  GTX.Zip's support package for BWA includes bwa-gtz and bwa-opt-gtz, both of which are based on version 0.7.17 of bwa. Among them: the two versions have added the ability to read GTZ files directly, and the functions are completely consistent with the main code functions of bwa. bwa-opt-gtz also optimizes the structure of BWA lookup table, which can save more than one third of the time without changing the results of comparison. Due to some changes in the data structure of the lookup table, bwa-opt-gtz is incompatible with the index file data generated by the original bwa. According to the standard steps of bwa, first regenerate the index file, and then compare it with bwa-opt-gtz.

  The difference between bwa-gtz and bwa-opt-gtz is as follows:

  (1) bwa-gtz can directly use index produced by official website BWA, and its performance is consistent with official website BWA.

  (2) bwa-opt-gtz can not directly use the index produced by BWA on official website. index needs to be reproduced by bwa-opt-gtz, but its performance will be improved by 1/3 than that of BWA on official website.

• Use examples

  bwa-gtz

  export GTZ_RBIN_PATH=/path/rbin

  bwa-gtz mem ref.fa read1.fq.gtz read2.fq.gtz -o aln-pe.sam

  * In this example, the path of the RBIN file is specified by the environment variable GTZ_RBIN_PATH, where "export GTZ_RBIN_PATH=/path/rbin" is not necessary, but if you know the path of rbin, you are advised to specify it, which can speed up the processing of bwa-gtz. Because when bwa-gtz needs RBIN file and cannot find the RBIN file under the default path ~/.config/gtz, it will be downloaded through the network, and the download process will consume time.

  bwa-opt-gtz

  Step one: Remake index (must)

  bwa-opt-gtz index ref.fa

  Step two: execution comparison

  export GTZ_RBIN_PATH=/path/rbin

  bwa-opt-gtz mem ref.fa read1.fq.gtz read2.fq.gtz -t 4 -o aln-pe.sam

• Performance

  In the case of sufficient server resources, the performance of bwa-opt-gtz is 1/3 better than that of official bwa. The following is a set of test data in the same environment (the number of specified threads is 4):

  Test command

  bwa mem ref.fa read1.fq.gz read2.fq.gz -t 4 -o aln-pe.sam

  bwa-gtz mem ref.fa read1.fq.gtz read2.fq.gtz -t 4 -o aln-pe.sam

  bwa-opt-gtz mem ref.fa read1.fq.gtz read2.fq.gtz -t 4 -o aln-pe.sam

  Test environment

  Server configuration: 16 core CPU, 64G memory; file size: read1.fq.gz(1.8G), read2.fq.gz(1.8G), read1.fq.gtz(0.3G), read2.fq.gtz(0.3G)

  performance data

  Software	bwa	bwa-gtz	bwa-opt-gtz
  Time consumption	50m14.06s	51m37.67s	39m18.86s
  Memory consumption	5.888G	10.56G	19.84G