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Conveyor-based analysis pipelines for metagenome data

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MGX-metagenomics/pipelines

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This is the central repository of public MGX pipelines.

Field description:

  • Name: Short pipeline name
  • Description: Pipeline description
  • Author: Pipeline maintainer
  • URL: Web site with additional pipeline information
  • Version: Pipeline version
  • File: Conveyor workflow for read-based analysis
  • File2: Conveyor workflow for gene annotation

All pipelines should use 0-based coordinates when creating sequence observations.

Taxonomic assignment

Name Description Author URL version file file2
MGX default taxonomy MGX default taxonomic classification based on Kraken and Diamond vs. RefSeq proteins Sebastian Jaenicke - 1.0 mgx_default_taxonomy.xml
Centrifuge Centrifuge: rapid and sensitive classification of metagenomic sequences Sebastian Jaenicke - 1.0 centrifuge.xml mgxgene_centrifuge.xml
Kaiju Kaiju: Fast and sensitive taxonomic classification for metagenomics Sebastian Jaenicke - 1.0 kaiju.xml mgxgene_kaiju.xml
Kraken Kraken: ultrafast metagenomic sequence classification using exact alignments Sebastian Jaenicke - 1.0 kraken.xml
KrakenUniq Taxonomic sequence classification with KrakenUniq Sebastian Jaenicke - 1.0 krakenuniq.xml
Kraken 2 Kraken: ultrafast metagenomic sequence classification using exact alignments Sebastian Jaenicke - 1.0 kraken2.xml mgxgene_kraken2.xml
MetaPhlAn 4 MetaPhlAn 4 marker-based taxonomic classification Sebastian Jaenicke - 1.0 metaphlan4.xml
16S Pipeline Classification of 16S rRNA fragments Sebastian Jaenicke 1.0 rdp_pipeline.xml

Functional profiling

Name Description Author URL version file file2
COG COG-based functional classification Sebastian Jaenicke - 1.0 eggnog.xml mgxgene_eggnog.xml
SwissProt EC numbers EC number annotation based on best-Blast-hit vs. SwissProt database Sebastian Jaenicke - 1.0 ecnumber.xml mgxgene_ecnumber.xml
FunGene HMM search HMM search vs. FunGene functional genes Sebastian Jaenicke - 1.0 fungene.xml mgxgene_fungene.xml
dbCAN Automated Carbohydrate-active enzyme annotation based on dbCAN Sebastian Jaenicke - 1.0 dbCAN_besthit.xml mgxgene_dbcan.xml
ClusterMine360 PKS/NRPS analysis based on BLAST vs ClusterMine360 database Sebastian Jaenicke - 1.0 clustermine360.xml mgxgene_clustermine360.xml
Pfam Annotate best Pfam domain hit Sebastian Jaenicke - 1.0 pfam_besthit.xml mgxgene_pfam.xml
TIGRFAMS Annotate best TIGRFAMS domain hit Sebastian Jaenicke - 1.0 tigrfam_besthit.xml mgxgene_tigrfam.xml
PGAP Annotate best NCBI PGAP HMM hit Sebastian Jaenicke - 1.0 pgap.xml mgxgene_pgap.xml
MIBiG MIBiG secondary metabolite screening Sebastian Jaenicke - 1.0 mibig.xml mgxgene_mibig.xml
KOfam KO number annotation Sebastian Jaenicke - 1.0 kofam.xml mgxgene_kofam.xml
UniRef50 UniRef50 annotation Sebastian Jaenicke - 1.0 uniref50.xml mgxgene_uniref50.xml
VOGDB Virus orthologous groups Sebastian Jaenicke - 1.0 - mgxgene_vogdb.xml

Antimicrobial resistance

Name Description Author URL version file file2
ARDB Antibiotic resistance gene annotation using best Blast hit vs. ARDB database Sebastian Jaenicke - 1.0 ardb.xml mgxgene_ardb.xml
ARG-Annot Antibiotic resistance gene annotation using best Blast hit vs. ARG-ANNOT database Sebastian Jaenicke - 1.0 argannot.xml
BacMet Antibacterial biocide- and metal-resistance gene annotation using best Blast hit vs. BacMet database Sebastian Jaenicke - 1.0 bacmet.xml mgxgene_bacmet.xml
CARD Antibiotic resistance gene screening using Blast vs CARD (Comprehensive antibiotic resistance database) Sebastian Jaenicke - 1.0 card.xml mgxgene_card.xml
MVirDB MvirDB-based virulence analysis Sebastian Jaenicke - 1.0 mvirdb.xml mgxgene_mvirdb.xml
AMRFinder+ NCBI AMRFinder+ Sebastian Jaenicke - 1.0 - mgxgene_amrfinder.xml

Reference mapping

Name Description Author URL version file
FR-HIT Fragment recruitment based on NCBI Magic-BLAST Sebastian Jaenicke - 1.0 blastn_refmap.xml
Bowtie2 Bowtie2-based reference mapping Sebastian Jaenicke - 1.0 bowtie_refmap.xml
Blast-Mapping Fragment recruitment employing FR-HIT Sebastian Jaenicke - 1.0 frhit_refmap.xml

Amplicon analysis

Name Description Author URL version file
16S-Amplicons Bergey RDP-based taxonomic assignment for 16S amplicons Burkhard Linke - 1.0 rdp_amplicons.xml

Misc

Name Description Author URL version file
Sebastian Jaenicke - 1.0 besthit_aa.xml
Sebastian Jaenicke - 1.0 discard_rRNAs.xml
Sebastian Jaenicke - 1.0 ecolifilter.xml
Sebastian Jaenicke - 1.0 phageprotscreen.xml
Sebastian Jaenicke - 1.0 phagescreen.xml
Sebastian Jaenicke - 1.0 pks.xml
Sebastian Jaenicke - 1.0 plasmidprotscreen.xml
Sebastian Jaenicke - 1.0 referencefilter.xml

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