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Fast and Accurate 3D PSF Computation for Fluorescence Microscopy

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MicroscPSF/MicroscPSF-Py

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MicroscPSF-Py

This is a Python implementation of the fast microscope PSF generation tool (using the Gibson-Lanni model). Technical details can be found here.

PyPI version

Install

PyPI

$ python -m pip install MicroscPSF-Py

Source

$ git clone https://github.com/MicroscPSF/MicroscPSF-Py.git
$ cd MicroscPSF-Py
$ python setup.py install

Usage

Please see the examples.ipynb Jupyter notebook.

Acknowledgements

  • Original algorithm Li, J., Xue, F., & Blu, T. (2017). Fast and accurate three-dimensional point spread function computation for fluorescence microscopy. JOSA A, 34(6), 1029-1034. link.

  • Python implementation by Kyle Douglass and Hazen Babcock.

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