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BrightEyes Time-tagging module: open-source hardware, a time to digital converter, multi channels, with a resolution of 30 ps designed for fluorescence scanning laser microscopy.

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Welcome to BrightEyes-TTM!

The BrightEyes-TTM is an open-source project which consists in a data-acquisition card able to implement the so-called photon time-tagging acquisition mode with a time resolution of ~30ps designed for microscopy and based on a commercial FPGA Xilinx Kintex-7.

The BrightEyes-TTM project born as an offshoot of the BrighEyes project founded by the ERC in 2018 (Consolidator Grant, N. 818699). The principal aim of the BrightEyes-TTM project is to give to any microscopy laboratory the possibility to implement and further develop single-photon microscopy. The second aim is to trigger the interest of the microscopy community, and establish the BrigthEyes-TTM as a new standard for single-photon laser scanning microscopy (LSM) experiments.

Reference: Rossetta, A., Slenders, E., Donato, M. et al. The BrightEyes-TTM as an open-source time-tagging module for democratising single-photon microscopy. Nat Commun 13, 7406 (2022). https://doi.org/10.1038/s41467-022-35064-0 Reference: Perego, E., Zappone, S., et al. Content-enriched fluorescence lifetime fluctuation spectroscopy to study bio-molecular condensate formation (2023) bioRxiv 2023.06.09.544221; doi: https://doi.org/10.1101/2023.06.09.544221


THE COMPLETE DOCUMENTATION IS AVAILABLE AT https://brighteyes-ttm.readthedocs.io/


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BrightEyes Time-tagging module: open-source hardware, a time to digital converter, multi channels, with a resolution of 30 ps designed for fluorescence scanning laser microscopy.

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