Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

iVar variants inconsistency #100

Open
gianfilippo opened this issue May 18, 2021 · 2 comments
Open

iVar variants inconsistency #100

gianfilippo opened this issue May 18, 2021 · 2 comments

Comments

@gianfilippo
Copy link

gianfilippo commented May 18, 2021

Describe the bug
iVar variants seem to show some inconsistency between -m 0 (default) and -m 10.
When called with -m 10 it calls a variant that is not called when using -m 0

To Reproduce
samtools mpileup -A -d 0 -Q 0 $BAM | ivar variants -p test0_variants -q 20 -t 0.1 -r $reference_genome
samtools mpileup -A -d 0 -Q 0 $BAM | ivar variants -p test10_variants -q 20 -t 0.1 -m 10 -r $reference_genome

Expected behavior
I may be wrong, but I would expect more PASS calls in the -m 0
Actually, probably this is not correct. I should rather say that I would NOT expect more PASS calls in the -m 10

@gkarthik
Copy link
Member

gkarthik commented Jun 8, 2021

Depending on the number of low quality variants, this would be hard to judge. Any chance you could please provide me with a sample file that I can test? Thanks!

@gianfilippo
Copy link
Author

sorry about the delay. I will upload a sample as soon as possible

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Development

No branches or pull requests

2 participants