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iVar miss-priming issues? #91
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Hi,
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Hi!
I am currently working on the IDseq metagenomics platform to identify mapped reads to SARS-CoV-2. Basically my project consists on the sequencing of all reads from a clinical samples with COVID-19, and assemble the viral genome. The wet lab protocol is from MSSPE, so I enrich the sequencing library preparation with 73 primers spanning the viral genome. To test the pipeline, I have uploaded negative controls (water controls with the primers), and iVar seems to report a few reads that partially match the primers (about 10, 20, and up to 200 reads). Could this be some sort of miss-priming issue? Would this be fixed by using the
ivar getmasked
? Or is this something else? (I am attaching a picture of how my results look in this pipeline)The IDSeq pipeline uses the following commands:
Thanks a lot for your help!
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