VDJviz is a web-based graphical user interface application that allows browsing and analyzing immune repertoire sequencing (RepSeq) data. It can be used to visualize results of MITCR, MIGEC, MIXCR and MIGMAP RepSeq processing software as well as popular IMGT/HighV-QUEST and ImmunoSEQ Analyzer services. VDJviz can be installed and used as a local server, alternatively you can use an online demo version available at https://vdjviz.cdr3.net/ which currently has an upload limit of 25 datasets each having size at most 10,000 clonotypes. The list of VDJviz features at a glance:
- Clonotype table browsing with V/D/J markup.
- CDR3 pattern matching for a single sample and across multiple samples with flexible filters.
- Spectratype, V-Spectratype, V-J usage and clonality analysis. Those can be compared side-by-side using the Compare panel.
- Summary statistics and rarefaction for multiple samples.
- Clonotype sharing across samples (many-to-many intersection) with flexible clonotype matching criteria.
- Uploaded data sharing.
VDJviz uses VDJtools API as a back-end. The software utilizes Play framework for running the server instance and state-of-art web graphics libraries such as D3js for visualization.
The most straightforward way to install VDJviz as a local server is to download the latest release package.
After downloading unzip the package wherever you want, but please avoid long paths and spaces (Windows version is especially sensitive to it).
You can find the server executable in bin/
directory. To set up the server:
- Run
vdjviz.bat
file (Windows) - Run
bash vdjviz -Dconfig.file=application.conf
in your console (Linux/Mac OS)
Wait until the server is started, and go to localhost:9000
URL in your browser to open VDJviz.
The user generator is enabled in the config by default, so you can login with vdjviz1@vdjviz.com
as an email and vdjviz1
as password,
To stop application just press Ctrl-C
at any time in console.
Troubleshooting. Note that an exception will be thrown in case the
9000
port is busy:org.jboss.netty.channel.ChannelException: Failed to bind to: /0.0.0.0:9000
. In order to fix it, either close the application that is using this port (in UNIX thelsof -i:9000
will give the processes that are using the port) or pass the-Dhttp.port=XXXX
(whereXXXX
is new port id) argument tovdjviz
shell script (UNIX) /vdjviz.bat
(Windows)
VDJviz server configuration can be performed by manually editing application.conf
file in the bin/
directory. The configuration file has the following fields:
-
application.secret
The secret key used in cryptographic hash functions. -
uploadPath
Specifies the path that will be used by VDJviz to store user's uploaded files. You can use '~' symbol as a shortcut for user home directory. Default:~/vdjviz/
-
maxFileSize
File size limit in kB Default:0
(no limit) -
maxFilesCount
Limit on the number of uploaded files per user. Default:0
(no limit) -
maxClonotypesCount
Limit on the number of clonotypes for each uploaded file. Default:0
(no limit) -
allowSharing
Disable or enable sharing feature Default:true
(enabled) -
maxSharedGroups
Maximum number of shared analyses per user. Default:0
(no limit) -
deleteAfter
Time period after which uploaded files are deleted from the server, in hours. Default:0
(never) -
applyNewLimitsToOldUsers
If set totrue
the server will automatically update limtis of all existing user accounts according to the ones specified in config. If set tofalse
, the limits will only be applied to newly created users. Default:true
-
createDefaultUsers
Specifies whether the server will generate some default user accounts with predefined emails and passwords, setting their emails to<nameDefaultUser><id>@vdjviz.com
(e.g.vdjviz1@vdjviz.com
) and passwords to<nameDefaultUser><id>
(e.g.vdjviz1
). Set this option tofalse
if you don't need this feature and prefer to use registration via SMTP. Default:true
-
nDefaultUsers
Number of default users to be created. Default:1
-
nameDefaultUser
Default user name prefix. Default:vdjviz
-
userManagementSystem
Enable user management (admin) panel for users with admin rights Default:true
-
userManagementSystemAccounts
Specifies the list of user accounts with predefined emails and passwords that will be granted admin rights. Theemail
andpassword
properties inapplication.conf
should be used to specify their credentials. -
db.default.url
Points to the path that will be used to store H2 database file. Default value:~/vdjviz/h2.db
Standalone version uses H2 Database for handling metadata by default, if you want to change H2 to another DBMS please see the corresponding Play documentation section You can also use this database to manually modify user limits. -
allowRegistration
Show the Register button in login screen. Default:false
-
allowChangePasswords
Show the Change Password button in login screen. Default:false
-
smtp.*
SMTP server configuration. If you don't want to use registration features, you can leavesmtp.*
fields empty and generate default users.