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ypriverol committed Aug 28, 2024
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15 changes: 7 additions & 8 deletions sdrf-proteomics/README.adoc
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Expand Up @@ -535,24 +535,23 @@ TIP: If the SDRF do not specified the proteomics data acquisition method as _com

You can find an example of a DIA experiment in the following link: https://github.com/bigbio/proteomics-sample-metadata/blob/master/annotated-projects/PXD018830/PXD018830-DIA.sdrf.tsv[DIA example]

[[dia]]
[[dia-ms1-scan]]
==== Data Independent Acquisition - Scan window limits

Additionally to the general _comment[proteomics data acquisition method]_ column, the SDRF-Proteomics file format allows to capture other properties for the DIA method. The following properties are RECOMMENDED for DIA:

- _comment[scan window lower limit]_
- _comment[scan window upper limit]_

The scan window lower and upper limits are the m/z range used for the DIA acquisition. The values are expressed in m/z units.
- _comment[MS1 scan range]_: The MS1 scan range is the m/z range used for the DIA acquisition. The values are expressed in m/z units.

Example:

|===
| | assay name | comment[scan window lower limit] | comment[scan window upper limit] | comment[data file]
|sample 1| run 1 | 400 m/z | 1200 m/z | FILE_R1.RAW
|sample 1| run 2 | 400 m/z | 1200 m/z | FILE_R2.RAW
|assay name | comment[MS1 scan range] | comment[data file]
|run 1 | 400m/z - 1200m/z | FILE_R1.RAW
|run 2 | 400m/z - 1200m/z | FILE_R2.RAW
|===

TIP: While the specification recommend to write the MS1 scan range as an interval (e.g. 400m/z - 1200m/z), it is also possible to write the MS1 scan range as a single value (e.g. 400m/z) using two columns for the lower and upper limits. In those cases you can write the lower limit in the _comment[scan window lower limit]_ and the uper limit in _comment[scan window upper limit]_

[[use-cases]]
== SDRF-Proteomics use-cases representation (templates)

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