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shared_scripts

filter_seqs_7.py

This script takes an aligned fasta file and returns a fast file with gap only columns removed, and all positions preceding the last start and following the earliest finish removed. If the resulting alignment is below a specified length sequences are removed from the alignment and the filter recalculated until a satisfactory length is reached.

python filter_seqs_7.py [aligned fasta]

trim_fastq_v2.py

This script is a lightweight trimmer. It takes in a forward fastq, reverse fastq, and index fastq from a barcoded Illumina run and trims to a specified mean quality score. Requires biopython.

python trim_fastq_v2.py [forward fastq] [reverse fastq] [index fastq]

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