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MEYE tool is a free and open-source tool to perform pupillometry in real-time (or on videos) in different subjects (human and mice) and in different experimental conditions (head-fixation, virtual reality, 2-photon calcium imaging ...). To access MEYE visit the page
Wait until the GUI and the CNN model are finished loading. Once loaded, you can also use MEYE offline without any internet connection. MEYE has been tested on up-to-date versions of Microsoft Edge, Mozilla Firefox and Google Chrome.