This repository contains code and data to enable combined analysis of the Drosophila Optic Lobe neurons annotated in the FAFB-FlyWire and Male CNS Optic Lobe datasets.
The most important output is this matching table summarising the cell type to cell type matches between the male and female datasets. This is organised using the Nern et al. cell types as a reference and identifying the corresponding Schlegel et al or Matsliah et al cell types. The great majority of these matches are 1-to-1 but some are more complex. For further details, please consult the methods of the Nern et al preprint.
As a convenience github allows you to query the matching spreadsheet and Neuroglancer scenes containing both datasets are available (sample), allowing direct comparison of matched neurons.
The repository contains the following main folders
- data Canonical data recommended for analysis
- extradata Additional files related to the above or captures from other sources for convenience.
Because annotations in both source datasets may still change and because our matching between the two datasets may still be refined, we will occasionally make substantive changes to this repository. Github's versioning means you can always go back to a previous tagged version or indeed any commit!
1.0: First release matching- Nern et al. Male Optic Lobe bioRxiv v2
- Neuprint optic-lobe:v1.0
- Matsliah et al. FlyWire optic lobe intrinsic neuron typing downloaded from Codex on 2024-04-24.
NB this was intended to correspond to v3 of the bioRxiv preprint (2024-04-15) but there appears to have been a time offset. - Schlegel et al. FlyWire cell types matching flywire_annotations github repo v2.0
For the matching in this repository, please cite:
- Nern et al. Connectome-driven neural inventory of a complete visual system
NB Click on the
button on bioRxiv to get your preferred citation manager format.
You will almost certainly also want citations for the underlying cell types/connectome data:
-
Nern et al. Connectome-driven neural inventory of a complete visual system
(male optic lobe cell types and connectome, one hemisphere) -
Matsliah et al. Neuronal “parts list” and wiring diagram for a visual system
(FlyWire: comprehensive cell types for female optic lobe intrinsic neurons, presently unilateral) -
Schlegel et al. Whole-brain annotation and multi-connectome cell typing quantifies circuit stereotypy in Drosophila
(FlyWire: all female brain cell types inc Visual Projection and Centrifugal Neurons and bilateral typing of major optic lobe intrinsic neurons; FlyWire connectome) -
Dorkenwald et al. Neuronal wiring diagram of an adult brain
(FlyWire connectome)
NB users of the FlyWire connectome should be sure to cite both Dorkenwald et al. and Schlegel et al. See https://codex.flywire.ai/about_flywire for further details.