sdm 2.17
sdm 2.17
Addresses mostly issues with PacBio amplicons that are completely warped (chimeras, duplications, inversions etc). I was quite surprised how much can go wrong, but a large fraction of these should be addressed leading to sometimes twice as many reads recovered. Further increased the scan range of primer removal, addressing fused amplicons and leading to much cleaner data.
As part of this update the barcode scanning was revised to make this faster and more strict. Can potentially lead to missing some "unclean" amplicons, but in the end currently sdm will do an even stricter quality filtering than previous versions.
Thanks to Mohammad Bahram, who helped with QC for this new sdm version.