Add epi-illumination strategies to SMLM course text

figures/focal-volume/README.md

@@ -0,0 +1 @@
+The focal volume of a microscope. This the volume within which a point source will appear in focus in an image. Point sources outside of this volume will appear out-of-focus. The axial extent of the focal volume is called the depth of focus.

texts/smlm-lab-course/refs.bib

@@ -168,3 +168,43 @@ @article{douglass-naturephotonics-2016
 	keywords = {smlm-course},
 	pages = {705--708},
 }
+
+@article{tokunaga-naturemethods-2008,
+	title = {Highly inclined thin illumination enables clear single-molecule imaging in cells},
+	volume = {5},
+	copyright = {2008 Springer Nature America, Inc.},
+	issn = {1548-7105},
+	url = {https://www.nature.com/articles/nmeth1171},
+	doi = {10.1038/nmeth1171},
+	abstract = {We describe a simple illumination method of fluorescence microscopy for molecular imaging. Illumination by a highly inclined and thin beam increases image intensity and decreases background intensity, yielding a signal/background ratio about eightfold greater than that of epi-illumination. A high ratio yielded clear single-molecule images and three-dimensional images using cultured mammalian cells, enabling one to visualize and quantify molecular dynamics, interactions and kinetics in cells for molecular systems biology.},
+	language = {en},
+	number = {2},
+	urldate = {2023-09-01},
+	journal = {Nature Methods},
+	author = {Tokunaga, Makio and Imamoto, Naoko and Sakata-Sogawa, Kumiko},
+	month = feb,
+	year = {2008},
+	note = {Number: 2
+Publisher: Nature Publishing Group},
+	keywords = {smlm-course},
+	pages = {159--161},
+}
+
+
+@article{axelrod-traffic-2001,
+	title = {Total {Internal} {Reflection} {Fluorescence} {Microscopy} in {Cell} {Biology}},
+	volume = {2},
+	issn = {1600-0854},
+	url = {https://onlinelibrary.wiley.com/doi/abs/10.1034/j.1600-0854.2001.21104.x},
+	doi = {10.1034/j.1600-0854.2001.21104.x},
+	abstract = {Key events in cellular trafficking occur at the cell surface, and it is desirable to visualize these events without interference from other regions deeper within. This review describes a microscopy technique based on total internal reflection fluorescence which is well suited for optical sectioning at cell-substrate regions with an unusually thin region of fluorescence excitation. The technique has many other applications as well, most notably for studying biochemical kinetics and single biomolecule dynamics at surfaces. A brief summary of these applications is provided, followed by presentations of the physical basis for the technique and the various ways to implement total internal reflection fluorescence in a standard fluorescence microscope.},
+	language = {en},
+	number = {11},
+	urldate = {2023-09-01},
+	journal = {Traffic},
+	author = {Axelrod, Daniel},
+	year = {2001},
+	note = {\_eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1034/j.1600-0854.2001.21104.x},
+	keywords = {smlm-course},
+	pages = {764--774},
+}