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Wang & McKenzie-Smith et al. Isolation disrupts social interactions and destabilizes brain development in bumblebees. In submission

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Bumblebee Isolation

Worker Brain Segmentation

Download Segmentation File

This file (.vtk) contains the manual annotations of the worker bumblebee brain atlas. It can be loaded into ITK-Snap and read out as segmentations of different neuropil regions


Worker Brain Template

Download Full Template

This file (.tif) contains the template for the worker bumblebee brain atlas. It can be used to register samples to, and can be opened in FIJI.


SLEAP Output

Download SLEAP Output

These files (.h5) contain the SLEAP output of body part locations and identity tracks for all trials.


Lab Frame Coordinates

Download Coordinates

This file (.mat) contains the lab frame coordinates for the top center of the arena for each behavior trial, to allow all bee tracks to be converted to the same coordinate system.


Body Posture Embedding

We used the technique described in "Mapping the stereotyped behaviour of freely-moving fruit flies” by Berman, GJ, Choi, DM, Bialek, W, and Shaevitz, JW, J. Royal Society Interface, 99, 20140672 (2014). See https://github.com/gordonberman/MotionMapper for details.


Compositional Data Analysis

Our composition data analysis is based on "Cerebellar contributions to a brainwide network for flexible behavior" by Verpeut, JL, Bergeler, S, Kislin, M, Townes, FW, Klibaite, U, Dhanerwala, ZM, Hoag, A, Jung, C, Lee, J, Pisano, TJ, Seagraves, KM, Shaevitz, JW, and Wang, SS-H, bioRxiv (2021). See https://github.com/PrincetonUniversity/OF-ymaze-cfos-analysis for details.

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Wang & McKenzie-Smith et al. Isolation disrupts social interactions and destabilizes brain development in bumblebees. In submission

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