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Programs and versions used | ||
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BUSCO 5.7.0 Gene prediction | ||
Dorodo 0.3.4 High accuracy rebasecalling | ||
Flye 2.9.1 De novo assembly | ||
GffToBed 1.0.3 Convert gff to bed | ||
Guppy 6.5.7 Initial Basecalling | ||
Kraken2 2.1.3 Contamination | ||
LASTZ 1.04.22 Alignment | ||
Medaka 1.9.1 Polishing | ||
Minimap2 2.14 Alignment | ||
MinKNOW 23.04.06 Nanopore sequencer software (Bream 7.5.10; Config 5.5.14; MinKNOW Core 5.5.5) | ||
MITOS2 2.1.3 Mitochondrial genome annotation | ||
Pod5 0.2.4 FAST5 to pod5 conversion | ||
QUAST 5.2.0 Assembly statistics | ||
Ragtag 2.1.0 Scaffolding | ||
RepeatMasker 4.1.5 Repetitive DNA identification | ||
SAMtools 1.3.1 SAM/BAM handling, coverage data | ||
SeqKit 2.5.1 FASTA handling | ||
Seqtk 1.4 FASTA handling | ||
TOGA 1.1.5 Gene Annotation | ||
UCSC-fatotwobit 447 FASTA to 2bit | ||
UCSC-genepredtobed 447 genePred to bed | ||
UCSC-gfftogenepred 447 GFF3 to genePred | ||
UCSC-gtftogenepred 447 GTF to genePred | ||
compleasm 0.2.5 annotation comparisions | ||
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