MAHELAB Standard Operating Procedures

Templates:

SOP Template

Enrichment:

RBC Lysis and Cell Pelleting of Blood and Bone Marrow
Tissue Transfer for Extraction

Extraction:

DNA Extraction from Cell Pellet Materials
RNA Extraction from Cell Pellet Materials
DNA Extraction from FFPE or Tissue Transfer Materials
RNA Extraction from FFPE or Tissue Transfer Materials
SDS-DTT Protein Extraction From FFPE Material
Acetone Protein Precipitation

Quantification:

Qubit hs dsDNA/RNA Quantification
Qubit Protein Quantification
Iron Extraction and Quantification
Human sCD30 Instant ELIZA Kit

PCR Protocols:

NEB Reverse Transcription Protocol

Electrophoresis:

BioMax Agarose Gel Electrophoresis
Agarose Band Excision
SDS-Page Protein Electrophoresis

Blotting:

Western Blot

Staining:

Western Blot SYPRO Total Protein Stain
Western Blot Fluorescence Immunostain

Imaging: